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Sample preparation using a miniatuarized supported liquid membrane device connected on-line to packed capillary liquid chromatography

Thordarson, Eddie; Pálmarsdóttir, Sveinbjörg; Mathiasson, Lennart LU and Jönsson, Jan Åke LU (1996) In Analytical Chemistry 68(15). p.2559-2563
Abstract
A miniaturized supported liquid membrane device has been developed for sample preparation and connected on-line to a packed capillary liquid chromatograph. The device consists of hydrophobic polypropylene hollow fiber, inserted and fastened in a cylindrical channel in a Kel-F piece. The pores of the fiber are filled with an organic solvent, in this study 6-undecanone, thus forming a liquid membrane. The sample is pumped on the outside of the hollow fiber (donor), and the analytes are selectively enriched and trapped in the fiber lumen (acceptor). With this approach, the volume of the acceptor solution can be kept as low as 1-2 L. This stagnant acceptor solution is then transferred through capillaries attached to the fiber ends to the LC... (More)
A miniaturized supported liquid membrane device has been developed for sample preparation and connected on-line to a packed capillary liquid chromatograph. The device consists of hydrophobic polypropylene hollow fiber, inserted and fastened in a cylindrical channel in a Kel-F piece. The pores of the fiber are filled with an organic solvent, in this study 6-undecanone, thus forming a liquid membrane. The sample is pumped on the outside of the hollow fiber (donor), and the analytes are selectively enriched and trapped in the fiber lumen (acceptor). With this approach, the volume of the acceptor solution can be kept as low as 1-2 L. This stagnant acceptor solution is then transferred through capillaries attached to the fiber ends to the LC system. The system was tested with a secondary amine (bambuterol), as a model substance in aqueous standard solutions as well as in plasma. The best extraction efficiency in aqueous solution, with an acceptor volume of 1.9 L, was 32.5% at a donor flow rate of 2.5 L/min. At flow rates above 20 L/min, the concentration enrichment per time unit was approximately constant, at 0.9 times/min, i.e., 9 times enrichment in about 10 min. The overall repeatability (RSD) for spiked plasma samples was ~4% (n = 12). Linear calibration curves of peak area versus bambuterol concentration were obtained for both aqueous standard solutions and spiked plasma samples. The detection limit for bambuterol in plasma, after 10 min of extraction at a flow rate of 24 L/min, was 80 nM. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Analytical Chemistry
volume
68
issue
15
pages
2559 - 2563
publisher
The American Chemical Society
external identifiers
  • scopus:0030174601
ISSN
1520-6882
DOI
10.1021/ac950929f
language
English
LU publication?
yes
id
6021916e-121e-4aae-bba5-186990d0ac6a (old id 31584)
date added to LUP
2007-06-19 14:37:10
date last changed
2017-01-01 04:24:58
@article{6021916e-121e-4aae-bba5-186990d0ac6a,
  abstract     = {A miniaturized supported liquid membrane device has been developed for sample preparation and connected on-line to a packed capillary liquid chromatograph. The device consists of hydrophobic polypropylene hollow fiber, inserted and fastened in a cylindrical channel in a Kel-F piece. The pores of the fiber are filled with an organic solvent, in this study 6-undecanone, thus forming a liquid membrane. The sample is pumped on the outside of the hollow fiber (donor), and the analytes are selectively enriched and trapped in the fiber lumen (acceptor). With this approach, the volume of the acceptor solution can be kept as low as 1-2 L. This stagnant acceptor solution is then transferred through capillaries attached to the fiber ends to the LC system. The system was tested with a secondary amine (bambuterol), as a model substance in aqueous standard solutions as well as in plasma. The best extraction efficiency in aqueous solution, with an acceptor volume of 1.9 L, was 32.5% at a donor flow rate of 2.5 L/min. At flow rates above 20 L/min, the concentration enrichment per time unit was approximately constant, at 0.9 times/min, i.e., 9 times enrichment in about 10 min. The overall repeatability (RSD) for spiked plasma samples was ~4% (n = 12). Linear calibration curves of peak area versus bambuterol concentration were obtained for both aqueous standard solutions and spiked plasma samples. The detection limit for bambuterol in plasma, after 10 min of extraction at a flow rate of 24 L/min, was 80 nM.},
  author       = {Thordarson, Eddie and Pálmarsdóttir, Sveinbjörg and Mathiasson, Lennart and Jönsson, Jan Åke},
  issn         = {1520-6882},
  language     = {eng},
  number       = {15},
  pages        = {2559--2563},
  publisher    = {The American Chemical Society},
  series       = {Analytical Chemistry},
  title        = {Sample preparation using a miniatuarized supported liquid membrane device connected on-line to packed capillary liquid chromatography},
  url          = {http://dx.doi.org/10.1021/ac950929f},
  volume       = {68},
  year         = {1996},
}