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Cellulose accessibility determines the rate of enzymatic hydrolysis of steam-pretreated spruce.

Wiman, Magnus LU ; Dienes, Dora D LU ; Hansen, Mads A T; van der Meulen, Torbjörn; Zacchi, Guido LU and Lidén, Gunnar LU (2012) In Bioresource Technology 126C. p.208-215
Abstract
Spruce chips steam-pretreated at various conditions, according to a central composite design, were used for investigating the influence of pretreatment conditions on enzymatic hydrolysis, accounting for the individual effects of pretreatment temperature (194-220°C), time (3-11min) and sulfur dioxide uptake (0.7-2.5%). The materials were analyzed for several surface characteristics, including IR absorption, enzyme adsorption capacity, total surface area, cellulosic surface area, and cellulosic pore sizes. This work showed a clear correlation between rate of enzymatic hydrolysis and specific surface area. Although the lignin content of the particle surface increased at higher pretreatment temperature and residence time, the initial rate of... (More)
Spruce chips steam-pretreated at various conditions, according to a central composite design, were used for investigating the influence of pretreatment conditions on enzymatic hydrolysis, accounting for the individual effects of pretreatment temperature (194-220°C), time (3-11min) and sulfur dioxide uptake (0.7-2.5%). The materials were analyzed for several surface characteristics, including IR absorption, enzyme adsorption capacity, total surface area, cellulosic surface area, and cellulosic pore sizes. This work showed a clear correlation between rate of enzymatic hydrolysis and specific surface area. Although the lignin content of the particle surface increased at higher pretreatment temperature and residence time, the initial rate of enzymatic hydrolysis increased. Enzyme adsorption measurements and staining methods revealed that the higher rate of hydrolysis of these materials was due to increased accessibility of the cellulose. An accessible cellulose fraction is thus more important than a low surface lignin content for the enzymatic hydrolysis of steam-pretreated spruce. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Bioresource Technology
volume
126C
pages
208 - 215
publisher
Elsevier
external identifiers
  • wos:000313997500031
  • pmid:23073110
  • scopus:84867294955
ISSN
1873-2976
DOI
10.1016/j.biortech.2012.08.082
language
English
LU publication?
yes
id
9d9f5578-9e5d-491a-8bb0-3bac3a2de9cc (old id 3160634)
date added to LUP
2012-11-13 11:29:55
date last changed
2017-11-19 03:51:16
@article{9d9f5578-9e5d-491a-8bb0-3bac3a2de9cc,
  abstract     = {Spruce chips steam-pretreated at various conditions, according to a central composite design, were used for investigating the influence of pretreatment conditions on enzymatic hydrolysis, accounting for the individual effects of pretreatment temperature (194-220°C), time (3-11min) and sulfur dioxide uptake (0.7-2.5%). The materials were analyzed for several surface characteristics, including IR absorption, enzyme adsorption capacity, total surface area, cellulosic surface area, and cellulosic pore sizes. This work showed a clear correlation between rate of enzymatic hydrolysis and specific surface area. Although the lignin content of the particle surface increased at higher pretreatment temperature and residence time, the initial rate of enzymatic hydrolysis increased. Enzyme adsorption measurements and staining methods revealed that the higher rate of hydrolysis of these materials was due to increased accessibility of the cellulose. An accessible cellulose fraction is thus more important than a low surface lignin content for the enzymatic hydrolysis of steam-pretreated spruce.},
  author       = {Wiman, Magnus and Dienes, Dora D and Hansen, Mads A T and van der Meulen, Torbjörn and Zacchi, Guido and Lidén, Gunnar},
  issn         = {1873-2976},
  language     = {eng},
  pages        = {208--215},
  publisher    = {Elsevier},
  series       = {Bioresource Technology},
  title        = {Cellulose accessibility determines the rate of enzymatic hydrolysis of steam-pretreated spruce.},
  url          = {http://dx.doi.org/10.1016/j.biortech.2012.08.082},
  volume       = {126C},
  year         = {2012},
}