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Bacterial and fungal markers in tobacco smoke.

Szponar, Bogumila LU ; Pehrson, Christina LU and Huss, Linnea LU (2012) In Science of the Total Environment 438(Nov.,1). p.447-451
Abstract
Previous research has demonstrated that cigarette smoke contains bacterial and fungal components including lipopolysaccharide (LPS) and ergosterol. In the present study we used gas chromatography-mass spectrometry to analyze tobacco as well as mainstream and second hand smoke for 3-hydroxy fatty acids (3-OH FAs) of 10 to 18 carbon chain lengths, used as LPS markers, and ergosterol, used as a marker of fungal biomass. The air concentrations of LPS were 0.0017nmol/m(3) (N=5) and 0.0007/m(3) (N=6) in the smoking vs. non-smoking rooms (p=0.0559) of the studied private houses, and 0.0231nmol/m(3) (N=5) vs. 0.0006nmol/m(3) (N=5) (p=0.0173), respectively, at the worksite. The air concentrations of ergosterol were also significantly higher in... (More)
Previous research has demonstrated that cigarette smoke contains bacterial and fungal components including lipopolysaccharide (LPS) and ergosterol. In the present study we used gas chromatography-mass spectrometry to analyze tobacco as well as mainstream and second hand smoke for 3-hydroxy fatty acids (3-OH FAs) of 10 to 18 carbon chain lengths, used as LPS markers, and ergosterol, used as a marker of fungal biomass. The air concentrations of LPS were 0.0017nmol/m(3) (N=5) and 0.0007/m(3) (N=6) in the smoking vs. non-smoking rooms (p=0.0559) of the studied private houses, and 0.0231nmol/m(3) (N=5) vs. 0.0006nmol/m(3) (N=5) (p=0.0173), respectively, at the worksite. The air concentrations of ergosterol were also significantly higher in rooms with ongoing smoking than in rooms without smoking. A positive correlation was found between LPS and ergosterol in rooms with smoking but not in rooms without smoking. 3-OH C14:0 was the main 3-OH FA, followed by 3-OH C12:0, both in mainstream and second hand smoke and in phenol:water smoke extracts prepared in order to purify the LPS. The Limulus activity of the phenolic phase of tobacco was 3900endotoxin units (EU)/cigarette; the corresponding amount of the smoke, collected on filters from 8 puffs, was 4EU/cigarette. Tobacco smoking has been associated with a range of inflammatory airway conditions including COPD, asthma, bronchitis, alveolar hypersensitivity etc. Significant levels of LPS and ergosterol were identified in tobacco smoke and these observations support the hypothesis that microbial components of tobacco smoke contribute to inflammation and airway disease. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Science of the Total Environment
volume
438
issue
Nov.,1
pages
447 - 451
publisher
Elsevier
external identifiers
  • wos:000313155300050
  • pmid:23026151
  • scopus:84866653053
ISSN
1879-1026
DOI
10.1016/j.scitotenv.2012.08.067
language
English
LU publication?
yes
id
463ae8af-04ac-4a06-8668-20f08d5cad55 (old id 3161350)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/23026151?dopt=Abstract
date added to LUP
2012-11-01 12:14:43
date last changed
2017-10-01 04:57:31
@article{463ae8af-04ac-4a06-8668-20f08d5cad55,
  abstract     = {Previous research has demonstrated that cigarette smoke contains bacterial and fungal components including lipopolysaccharide (LPS) and ergosterol. In the present study we used gas chromatography-mass spectrometry to analyze tobacco as well as mainstream and second hand smoke for 3-hydroxy fatty acids (3-OH FAs) of 10 to 18 carbon chain lengths, used as LPS markers, and ergosterol, used as a marker of fungal biomass. The air concentrations of LPS were 0.0017nmol/m(3) (N=5) and 0.0007/m(3) (N=6) in the smoking vs. non-smoking rooms (p=0.0559) of the studied private houses, and 0.0231nmol/m(3) (N=5) vs. 0.0006nmol/m(3) (N=5) (p=0.0173), respectively, at the worksite. The air concentrations of ergosterol were also significantly higher in rooms with ongoing smoking than in rooms without smoking. A positive correlation was found between LPS and ergosterol in rooms with smoking but not in rooms without smoking. 3-OH C14:0 was the main 3-OH FA, followed by 3-OH C12:0, both in mainstream and second hand smoke and in phenol:water smoke extracts prepared in order to purify the LPS. The Limulus activity of the phenolic phase of tobacco was 3900endotoxin units (EU)/cigarette; the corresponding amount of the smoke, collected on filters from 8 puffs, was 4EU/cigarette. Tobacco smoking has been associated with a range of inflammatory airway conditions including COPD, asthma, bronchitis, alveolar hypersensitivity etc. Significant levels of LPS and ergosterol were identified in tobacco smoke and these observations support the hypothesis that microbial components of tobacco smoke contribute to inflammation and airway disease.},
  author       = {Szponar, Bogumila and Pehrson, Christina and Huss, Linnea},
  issn         = {1879-1026},
  language     = {eng},
  number       = {Nov.,1},
  pages        = {447--451},
  publisher    = {Elsevier},
  series       = {Science of the Total Environment},
  title        = {Bacterial and fungal markers in tobacco smoke.},
  url          = {http://dx.doi.org/10.1016/j.scitotenv.2012.08.067},
  volume       = {438},
  year         = {2012},
}