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Analysis of photon count data from single-molecule fluorescence experiments

Burzykowski, T; Szubiakowski, J and Rydén, Tobias LU (2003) In Chemical Physics 288(2-3). p.291-307
Abstract
We consider single-molecule fluorescence experiments with data in the form of counts of photons registered over multiple time-intervals. Based on the observation schemes, linking back to works by Dehmelt [Bull. Am. Phys. Soc. 20 (1975) 60] and Cook and Kimble [Phys. Rev. Lett. 54 (1985) 1023], we propose an analytical approach to the data based on the theory of Markov-modulated Poisson processes (MMPP). In particular, we consider maximum-likelihood estimation. The method is illustrated using a real-life dataset. Additionally, the properties of the proposed method are investigated through simulations and compared to two other approaches developed by Yip et al. [J. Phys. Chem. A 102 (1998) 7564] and Molski [Chem. Phys. Lett. 324 (2000) 301].
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
hyperexponential distribution, Poisson process, Markov-modulated, single-molecule studies, fluorescence spectroscopy
in
Chemical Physics
volume
288
issue
2-3
pages
291 - 307
publisher
Elsevier
external identifiers
  • wos:000181550500016
  • scopus:0037443901
ISSN
0301-0104
DOI
10.1016/S0301-0104(03)00034-X
language
English
LU publication?
yes
id
7737baaa-62ab-4bed-83e0-0847c27523da (old id 316300)
date added to LUP
2007-08-02 13:24:02
date last changed
2018-05-29 09:22:23
@article{7737baaa-62ab-4bed-83e0-0847c27523da,
  abstract     = {We consider single-molecule fluorescence experiments with data in the form of counts of photons registered over multiple time-intervals. Based on the observation schemes, linking back to works by Dehmelt [Bull. Am. Phys. Soc. 20 (1975) 60] and Cook and Kimble [Phys. Rev. Lett. 54 (1985) 1023], we propose an analytical approach to the data based on the theory of Markov-modulated Poisson processes (MMPP). In particular, we consider maximum-likelihood estimation. The method is illustrated using a real-life dataset. Additionally, the properties of the proposed method are investigated through simulations and compared to two other approaches developed by Yip et al. [J. Phys. Chem. A 102 (1998) 7564] and Molski [Chem. Phys. Lett. 324 (2000) 301].},
  author       = {Burzykowski, T and Szubiakowski, J and Rydén, Tobias},
  issn         = {0301-0104},
  keyword      = {hyperexponential distribution,Poisson process,Markov-modulated,single-molecule studies,fluorescence spectroscopy},
  language     = {eng},
  number       = {2-3},
  pages        = {291--307},
  publisher    = {Elsevier},
  series       = {Chemical Physics},
  title        = {Analysis of photon count data from single-molecule fluorescence experiments},
  url          = {http://dx.doi.org/10.1016/S0301-0104(03)00034-X},
  volume       = {288},
  year         = {2003},
}