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Electrochemically induced oxidative damage to double-stranded calf thymus DNA adsorbed on gold electrodes

Ferapontova, Elena LU and Shipovskov, SV (2003) In Biochemistry 68(1). p.99-104
Abstract
Electrochemically induced oxidative damage to DNA was studied with double-stranded calf thymus DNA immobilized directly on a gold electrode surface. Pre-polarization of the DNA-modified electrodes at +0.5 V versus Ag/AgCl reference electrode, in a free from DNA blank buffer solution, pH 7.4, allowed for subsequent detection of direct electrochemical oxidation of adsorbed on gold DNA, in the potential range from +0.7 to +0.8 V. The redox potential of the process corresponded to the potentials of the oxidation of guanine bases in DNA. It is shown that with increasing potential scan rate, v, the mechanism of electrochemical oxidation of DNA changes from the irreversible 4e(-) oxidative damage of DNA at low v to reversible 1e(-) oxidation at... (More)
Electrochemically induced oxidative damage to DNA was studied with double-stranded calf thymus DNA immobilized directly on a gold electrode surface. Pre-polarization of the DNA-modified electrodes at +0.5 V versus Ag/AgCl reference electrode, in a free from DNA blank buffer solution, pH 7.4, allowed for subsequent detection of direct electrochemical oxidation of adsorbed on gold DNA, in the potential range from +0.7 to +0.8 V. The redox potential of the process corresponded to the potentials of the oxidation of guanine bases in DNA. It is shown that with increasing potential scan rate, v, the mechanism of electrochemical oxidation of DNA changes from the irreversible 4e(-) oxidative damage of DNA at low v to reversible 1e(-) oxidation at high nu, keeping the electrochemical activity of the adsorbed DNA layer virtually the same. (Less)
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author
and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
gold electrode, oxidative DNA damage, guanine
in
Biochemistry
volume
68
issue
1
pages
99 - 104
publisher
The American Chemical Society (ACS)
external identifiers
  • pmid:12693982
  • wos:000181471600011
  • scopus:0037243450
ISSN
0006-2960
DOI
10.1023/A:1022153720508
language
English
LU publication?
yes
additional info
The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Analytical Chemistry (S/LTH) (011001004)
id
0b28117a-d93e-4681-a639-4651e077f2c1 (old id 317078)
date added to LUP
2016-04-01 16:26:10
date last changed
2021-03-31 03:56:12
@article{0b28117a-d93e-4681-a639-4651e077f2c1,
  abstract     = {Electrochemically induced oxidative damage to DNA was studied with double-stranded calf thymus DNA immobilized directly on a gold electrode surface. Pre-polarization of the DNA-modified electrodes at +0.5 V versus Ag/AgCl reference electrode, in a free from DNA blank buffer solution, pH 7.4, allowed for subsequent detection of direct electrochemical oxidation of adsorbed on gold DNA, in the potential range from +0.7 to +0.8 V. The redox potential of the process corresponded to the potentials of the oxidation of guanine bases in DNA. It is shown that with increasing potential scan rate, v, the mechanism of electrochemical oxidation of DNA changes from the irreversible 4e(-) oxidative damage of DNA at low v to reversible 1e(-) oxidation at high nu, keeping the electrochemical activity of the adsorbed DNA layer virtually the same.},
  author       = {Ferapontova, Elena and Shipovskov, SV},
  issn         = {0006-2960},
  language     = {eng},
  number       = {1},
  pages        = {99--104},
  publisher    = {The American Chemical Society (ACS)},
  series       = {Biochemistry},
  title        = {Electrochemically induced oxidative damage to double-stranded calf thymus DNA adsorbed on gold electrodes},
  url          = {http://dx.doi.org/10.1023/A:1022153720508},
  doi          = {10.1023/A:1022153720508},
  volume       = {68},
  year         = {2003},
}