Electrochemically induced oxidative damage to double-stranded calf thymus DNA adsorbed on gold electrodes
(2003) In Biochemistry 68(1). p.99-104- Abstract
- Electrochemically induced oxidative damage to DNA was studied with double-stranded calf thymus DNA immobilized directly on a gold electrode surface. Pre-polarization of the DNA-modified electrodes at +0.5 V versus Ag/AgCl reference electrode, in a free from DNA blank buffer solution, pH 7.4, allowed for subsequent detection of direct electrochemical oxidation of adsorbed on gold DNA, in the potential range from +0.7 to +0.8 V. The redox potential of the process corresponded to the potentials of the oxidation of guanine bases in DNA. It is shown that with increasing potential scan rate, v, the mechanism of electrochemical oxidation of DNA changes from the irreversible 4e(-) oxidative damage of DNA at low v to reversible 1e(-) oxidation at... (More)
- Electrochemically induced oxidative damage to DNA was studied with double-stranded calf thymus DNA immobilized directly on a gold electrode surface. Pre-polarization of the DNA-modified electrodes at +0.5 V versus Ag/AgCl reference electrode, in a free from DNA blank buffer solution, pH 7.4, allowed for subsequent detection of direct electrochemical oxidation of adsorbed on gold DNA, in the potential range from +0.7 to +0.8 V. The redox potential of the process corresponded to the potentials of the oxidation of guanine bases in DNA. It is shown that with increasing potential scan rate, v, the mechanism of electrochemical oxidation of DNA changes from the irreversible 4e(-) oxidative damage of DNA at low v to reversible 1e(-) oxidation at high nu, keeping the electrochemical activity of the adsorbed DNA layer virtually the same. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/317078
- author
- Ferapontova, Elena LU and Shipovskov, SV
- organization
- publishing date
- 2003
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- gold electrode, oxidative DNA damage, guanine
- in
- Biochemistry
- volume
- 68
- issue
- 1
- pages
- 99 - 104
- publisher
- The American Chemical Society (ACS)
- external identifiers
-
- pmid:12693982
- wos:000181471600011
- scopus:0037243450
- ISSN
- 0006-2960
- DOI
- 10.1023/A:1022153720508
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Analytical Chemistry (S/LTH) (011001004)
- id
- 0b28117a-d93e-4681-a639-4651e077f2c1 (old id 317078)
- date added to LUP
- 2016-04-01 16:26:10
- date last changed
- 2022-02-05 08:10:37
@article{0b28117a-d93e-4681-a639-4651e077f2c1, abstract = {{Electrochemically induced oxidative damage to DNA was studied with double-stranded calf thymus DNA immobilized directly on a gold electrode surface. Pre-polarization of the DNA-modified electrodes at +0.5 V versus Ag/AgCl reference electrode, in a free from DNA blank buffer solution, pH 7.4, allowed for subsequent detection of direct electrochemical oxidation of adsorbed on gold DNA, in the potential range from +0.7 to +0.8 V. The redox potential of the process corresponded to the potentials of the oxidation of guanine bases in DNA. It is shown that with increasing potential scan rate, v, the mechanism of electrochemical oxidation of DNA changes from the irreversible 4e(-) oxidative damage of DNA at low v to reversible 1e(-) oxidation at high nu, keeping the electrochemical activity of the adsorbed DNA layer virtually the same.}}, author = {{Ferapontova, Elena and Shipovskov, SV}}, issn = {{0006-2960}}, keywords = {{gold electrode; oxidative DNA damage; guanine}}, language = {{eng}}, number = {{1}}, pages = {{99--104}}, publisher = {{The American Chemical Society (ACS)}}, series = {{Biochemistry}}, title = {{Electrochemically induced oxidative damage to double-stranded calf thymus DNA adsorbed on gold electrodes}}, url = {{http://dx.doi.org/10.1023/A:1022153720508}}, doi = {{10.1023/A:1022153720508}}, volume = {{68}}, year = {{2003}}, }