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None of the integrins known to be present on the mouse egg or to be ADAM receptors are essential for sperm-egg binding and fusion

He, ZY; Brakebusch, Cord LU ; Fassler, R; Kreidberg, JA; Primakoff, P and Myles, DG (2003) In Developmental Biology 254(2). p.226-237
Abstract
Antibody inhibition and alpha6beta1 ligand binding experiments indicate that the egg integrin alpha6beta1 functions as a receptor for sperm during gamete fusion; yet, eggs null for the alpha6 integrin exhibit normal fertilization. Alternative integrins may be involved in sperm-egg binding and fusion and could compensate for the absence of alpha6beta1. Various beta1 integrins and alphav integrins are present on mouse eggs. Some of these integrins are also reported to be receptors for ADAMs, which are expressed on sperm. Using alpha3 integrin null eggs, we found that the alpha3beta1 integrin was not essential for sperm-egg binding and fusion. Oocyte-specific, beta1 integrin conditional knockout mice allowed us to obtain mature eggs lacking... (More)
Antibody inhibition and alpha6beta1 ligand binding experiments indicate that the egg integrin alpha6beta1 functions as a receptor for sperm during gamete fusion; yet, eggs null for the alpha6 integrin exhibit normal fertilization. Alternative integrins may be involved in sperm-egg binding and fusion and could compensate for the absence of alpha6beta1. Various beta1 integrins and alphav integrins are present on mouse eggs. Some of these integrins are also reported to be receptors for ADAMs, which are expressed on sperm. Using alpha3 integrin null eggs, we found that the alpha3beta1 integrin was not essential for sperm-egg binding and fusion. Oocyte-specific, beta1 integrin conditional knockout mice allowed us to obtain mature eggs lacking all beta1 integrins. We found that the beta1 integrin null eggs were fully functional in fertilization both in vivo and in vitro. Furthermore, neither anti-mouse beta3 integrin function-blocking monoclonal antibody (mAb) nor alphav integrin function-blocking mAb inhibited sperm binding to or fusion with beta1 integrin null eggs. Thus, function of beta3 or alphav integrins does not seem to be involved in compensating for the absence of beta1 integrins. These results indicate that none of the integrins known to be present on mouse eggs or to be ADAM receptors are essential for sperm-egg binding/fusion, and thus, egg integrins may not play the role in gamete fusion previously attributed to them. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
null mutation, membrane fusion, integrin, sperm-egg binding, ADAMs
in
Developmental Biology
volume
254
issue
2
pages
226 - 237
publisher
Elsevier
external identifiers
  • pmid:12591243
  • wos:000181223100006
  • scopus:0037442567
ISSN
1095-564X
DOI
language
English
LU publication?
yes
id
785761d9-4fc1-4807-88c8-ac9065c72089 (old id 317911)
date added to LUP
2007-09-13 07:40:24
date last changed
2018-05-29 09:39:20
@article{785761d9-4fc1-4807-88c8-ac9065c72089,
  abstract     = {Antibody inhibition and alpha6beta1 ligand binding experiments indicate that the egg integrin alpha6beta1 functions as a receptor for sperm during gamete fusion; yet, eggs null for the alpha6 integrin exhibit normal fertilization. Alternative integrins may be involved in sperm-egg binding and fusion and could compensate for the absence of alpha6beta1. Various beta1 integrins and alphav integrins are present on mouse eggs. Some of these integrins are also reported to be receptors for ADAMs, which are expressed on sperm. Using alpha3 integrin null eggs, we found that the alpha3beta1 integrin was not essential for sperm-egg binding and fusion. Oocyte-specific, beta1 integrin conditional knockout mice allowed us to obtain mature eggs lacking all beta1 integrins. We found that the beta1 integrin null eggs were fully functional in fertilization both in vivo and in vitro. Furthermore, neither anti-mouse beta3 integrin function-blocking monoclonal antibody (mAb) nor alphav integrin function-blocking mAb inhibited sperm binding to or fusion with beta1 integrin null eggs. Thus, function of beta3 or alphav integrins does not seem to be involved in compensating for the absence of beta1 integrins. These results indicate that none of the integrins known to be present on mouse eggs or to be ADAM receptors are essential for sperm-egg binding/fusion, and thus, egg integrins may not play the role in gamete fusion previously attributed to them.},
  author       = {He, ZY and Brakebusch, Cord and Fassler, R and Kreidberg, JA and Primakoff, P and Myles, DG},
  issn         = {1095-564X},
  keyword      = {null mutation,membrane fusion,integrin,sperm-egg binding,ADAMs},
  language     = {eng},
  number       = {2},
  pages        = {226--237},
  publisher    = {Elsevier},
  series       = {Developmental Biology},
  title        = {None of the integrins known to be present on the mouse egg or to be ADAM receptors are essential for sperm-egg binding and fusion},
  url          = {http://dx.doi.org/},
  volume       = {254},
  year         = {2003},
}