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Independent prognostic value of angiogenesis and the level of plasminogen activator inhibitor type I in breast cancer patients

Hansen, S; Overgaard, J; Rose, Carsten LU ; Knoop, A; Laenkholm, AV; Andersen, J; Sorensen, FB and Andreasen, PA (2003) In British Journal of Cancer 88(1). p.102-108
Abstract
Tumour angiogenesis and the levels of plasminogen activator inhibitor type I (PAI-I) are both informative prognostic markers in breast cancer. In cell cultures and in animal model systems, PAI-I has a proangiogenic effect. To evaluate the interrelationship of angiogenesis and the PAI-I level in breast cancer, we have evaluated the prognostic value of those factors in a total of 228 patients with primary, unilateral, invasive breast cancer, evaluated at a median follow-up time of 12 years. Microvessels were immunohistochemically stained by antibodies against CD34 and quantitated by the Chalkley counting technique. The levels of PAI-I and its target proteinase uPA in tumour extracts were analysed by ELISA. The Chalkley count was not... (More)
Tumour angiogenesis and the levels of plasminogen activator inhibitor type I (PAI-I) are both informative prognostic markers in breast cancer. In cell cultures and in animal model systems, PAI-I has a proangiogenic effect. To evaluate the interrelationship of angiogenesis and the PAI-I level in breast cancer, we have evaluated the prognostic value of those factors in a total of 228 patients with primary, unilateral, invasive breast cancer, evaluated at a median follow-up time of 12 years. Microvessels were immunohistochemically stained by antibodies against CD34 and quantitated by the Chalkley counting technique. The levels of PAI-I and its target proteinase uPA in tumour extracts were analysed by ELISA. The Chalkley count was not correlated with the levels of uPA or PAI-I. High values of uPA, PAI-I, and Chalkley count were all significantly correlated with a shorter recurrence-free survival and overall survival. In the multivariate analysis, the uPA level did not show independent prognostic impact for any of the analysed end points. In contrast, the risk of recurrence was independently and significantly predicted by both the PAI-I level and the Chalkley count, with a hazard ratio (95% CI) of 1.6 (1.01-2.69) and 1.4 (1.02-1.81), respectively. For overall survival, the Chalkley count, but not PAI-I, was of significant independent prognostic value. The risk of death was 1.7 (1,30-2.15) for Chalkley counts in the upper tertile compared to the lower one. We conclude that the PAI-I level and the Chalkley count are independent prognostic markers for recurrence-free survival in patients with primary breast cancer, suggesting that the prognostic impact of PAI-I is not only based on its involvement in angiogenesis. (C) 2003 Cancer Research UK. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
prognosis, breast-neoplasms, neovascularization, survival-analysis
in
British Journal of Cancer
volume
88
issue
1
pages
102 - 108
publisher
Nature Publishing Group
external identifiers
  • pmid:12556967
  • wos:000181078900018
  • scopus:0037434421
ISSN
1532-1827
DOI
10.1038/sj.bjc.6600662
language
English
LU publication?
yes
id
d6203995-2932-48c0-a1db-41ae0973c906 (old id 317930)
date added to LUP
2007-09-03 14:50:25
date last changed
2018-10-03 10:04:11
@article{d6203995-2932-48c0-a1db-41ae0973c906,
  abstract     = {Tumour angiogenesis and the levels of plasminogen activator inhibitor type I (PAI-I) are both informative prognostic markers in breast cancer. In cell cultures and in animal model systems, PAI-I has a proangiogenic effect. To evaluate the interrelationship of angiogenesis and the PAI-I level in breast cancer, we have evaluated the prognostic value of those factors in a total of 228 patients with primary, unilateral, invasive breast cancer, evaluated at a median follow-up time of 12 years. Microvessels were immunohistochemically stained by antibodies against CD34 and quantitated by the Chalkley counting technique. The levels of PAI-I and its target proteinase uPA in tumour extracts were analysed by ELISA. The Chalkley count was not correlated with the levels of uPA or PAI-I. High values of uPA, PAI-I, and Chalkley count were all significantly correlated with a shorter recurrence-free survival and overall survival. In the multivariate analysis, the uPA level did not show independent prognostic impact for any of the analysed end points. In contrast, the risk of recurrence was independently and significantly predicted by both the PAI-I level and the Chalkley count, with a hazard ratio (95% CI) of 1.6 (1.01-2.69) and 1.4 (1.02-1.81), respectively. For overall survival, the Chalkley count, but not PAI-I, was of significant independent prognostic value. The risk of death was 1.7 (1,30-2.15) for Chalkley counts in the upper tertile compared to the lower one. We conclude that the PAI-I level and the Chalkley count are independent prognostic markers for recurrence-free survival in patients with primary breast cancer, suggesting that the prognostic impact of PAI-I is not only based on its involvement in angiogenesis. (C) 2003 Cancer Research UK.},
  author       = {Hansen, S and Overgaard, J and Rose, Carsten and Knoop, A and Laenkholm, AV and Andersen, J and Sorensen, FB and Andreasen, PA},
  issn         = {1532-1827},
  keyword      = {prognosis,breast-neoplasms,neovascularization,survival-analysis},
  language     = {eng},
  number       = {1},
  pages        = {102--108},
  publisher    = {Nature Publishing Group},
  series       = {British Journal of Cancer},
  title        = {Independent prognostic value of angiogenesis and the level of plasminogen activator inhibitor type I in breast cancer patients},
  url          = {http://dx.doi.org/10.1038/sj.bjc.6600662},
  volume       = {88},
  year         = {2003},
}