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Actomyosin motility on nanostructured surfaces

Bunk, Richard LU ; Klinth, J; Montelius, Lars LU ; Nicholls, IA; Omling, Pär LU ; Tagerud, S and Mansson, A (2003) In Biochemical and Biophysical Research Communications 301(3). p.783-788
Abstract
We have here, for the first time, used nanofabrication techniques to reproduce aspects of the ordered actomyosin arrangement in a muscle cell. The adsorption of functional heavy meromyosin (HMM) to five different resist polymers was first assessed. One group of resists (MRL-6000.1XP and ZEP-520) consistently exhibited high quality motility of actin filaments after incubation with HMM. A second group (PMMA-200, PMMA-950, and MRI-9030) generally gave low quality of motility with only few smoothly moving filaments. Based on these findings electron beam lithography was applied to a bi-layer resist system with PMMA-950 on top of MRL-6000.1XP. Grooves (100-200 nm wide) in the PMMA layer were created to expose the MRL-6000.1XP surface for... (More)
We have here, for the first time, used nanofabrication techniques to reproduce aspects of the ordered actomyosin arrangement in a muscle cell. The adsorption of functional heavy meromyosin (HMM) to five different resist polymers was first assessed. One group of resists (MRL-6000.1XP and ZEP-520) consistently exhibited high quality motility of actin filaments after incubation with HMM. A second group (PMMA-200, PMMA-950, and MRI-9030) generally gave low quality of motility with only few smoothly moving filaments. Based on these findings electron beam lithography was applied to a bi-layer resist system with PMMA-950 on top of MRL-6000.1XP. Grooves (100-200 nm wide) in the PMMA layer were created to expose the MRL-6000.1XP surface for adsorption of HMM and guidance of actin filament motility. This guidance was quite efficient allowing no U-turns of the filaments and approximately 20 times higher density of moving filaments in the grooves than on the surrounding PMMA. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
beam lithography, electron, nanotechnology, resist polymer, motility assay, actin, myosin, atomic force microscope
in
Biochemical and Biophysical Research Communications
volume
301
issue
3
pages
783 - 788
publisher
Elsevier
external identifiers
  • pmid:12565849
  • wos:000181143800025
  • scopus:0037436227
ISSN
1090-2104
DOI
10.1016/S0006-291X(03)00027-5
language
English
LU publication?
yes
id
85b4439c-e610-428e-a310-8667b954b428 (old id 317965)
date added to LUP
2007-08-02 13:19:33
date last changed
2017-07-23 04:42:46
@article{85b4439c-e610-428e-a310-8667b954b428,
  abstract     = {We have here, for the first time, used nanofabrication techniques to reproduce aspects of the ordered actomyosin arrangement in a muscle cell. The adsorption of functional heavy meromyosin (HMM) to five different resist polymers was first assessed. One group of resists (MRL-6000.1XP and ZEP-520) consistently exhibited high quality motility of actin filaments after incubation with HMM. A second group (PMMA-200, PMMA-950, and MRI-9030) generally gave low quality of motility with only few smoothly moving filaments. Based on these findings electron beam lithography was applied to a bi-layer resist system with PMMA-950 on top of MRL-6000.1XP. Grooves (100-200 nm wide) in the PMMA layer were created to expose the MRL-6000.1XP surface for adsorption of HMM and guidance of actin filament motility. This guidance was quite efficient allowing no U-turns of the filaments and approximately 20 times higher density of moving filaments in the grooves than on the surrounding PMMA.},
  author       = {Bunk, Richard and Klinth, J and Montelius, Lars and Nicholls, IA and Omling, Pär and Tagerud, S and Mansson, A},
  issn         = {1090-2104},
  keyword      = {beam lithography,electron,nanotechnology,resist polymer,motility assay,actin,myosin,atomic force microscope},
  language     = {eng},
  number       = {3},
  pages        = {783--788},
  publisher    = {Elsevier},
  series       = {Biochemical and Biophysical Research Communications},
  title        = {Actomyosin motility on nanostructured surfaces},
  url          = {http://dx.doi.org/10.1016/S0006-291X(03)00027-5},
  volume       = {301},
  year         = {2003},
}