Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

An agarose gel electrophoresis method for the separation of arabinogalactan proteins.

Stöhr, Christine ; Snogerup, Lars ; Pennell, Roger and Kjellbom, Per LU (1996) In Plant Journal 10(5). p.943-948
Abstract
A method for resolving plasma membrane associated arabinogalactan proteins (AGPs) has been developed. Plasma membranes purified by aqueous polymer two‐phase partitioning were first subjected to Triton X‐114 fractionation. The resulting water phase contained all detectable plasma membrane‐bound AGPs. Plasma membrane AGPs were then resolved in an SDS‐agarose gel electrophoresis system (SDS‐AGE). For separating plasma membrane AGP species of the same apparent molecular weight but with different net charge, a two‐dimensional electrophoresis system was used, utilizing isoelectric focusing in an immobilized pH gradient in the first dimension and SDS‐AGE in the second dimension. These methods enabled the separation of individual plasma membrane... (More)
A method for resolving plasma membrane associated arabinogalactan proteins (AGPs) has been developed. Plasma membranes purified by aqueous polymer two‐phase partitioning were first subjected to Triton X‐114 fractionation. The resulting water phase contained all detectable plasma membrane‐bound AGPs. Plasma membrane AGPs were then resolved in an SDS‐agarose gel electrophoresis system (SDS‐AGE). For separating plasma membrane AGP species of the same apparent molecular weight but with different net charge, a two‐dimensional electrophoresis system was used, utilizing isoelectric focusing in an immobilized pH gradient in the first dimension and SDS‐AGE in the second dimension. These methods enabled the separation of individual plasma membrane AGPs. In comparison, SDS‐PAGE methods left AGPs as unresolved high molecular‐weight smears. The methods described here may help to establish some basic features of AGPs, such as the number, organization, and protein and carbohydrate characteristics of plasma membrane AGPs, as well as the relationship between plasma membrane and extracellular AGPs. (Less)
Please use this url to cite or link to this publication:
author
; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Plant Journal
volume
10
issue
5
pages
943 - 948
publisher
Wiley-Blackwell
external identifiers
  • scopus:0030484283
ISSN
1365-313X
DOI
10.1046/j.1365-313X.1996.10050943.x
language
English
LU publication?
yes
id
3186b420-5b15-421b-9132-025cdaa147ce
date added to LUP
2019-06-20 17:10:38
date last changed
2023-06-18 04:01:11
@article{3186b420-5b15-421b-9132-025cdaa147ce,
  abstract     = {{A method for resolving plasma membrane associated arabinogalactan proteins (AGPs) has been developed. Plasma membranes purified by aqueous polymer two‐phase partitioning were first subjected to Triton X‐114 fractionation. The resulting water phase contained all detectable plasma membrane‐bound AGPs. Plasma membrane AGPs were then resolved in an SDS‐agarose gel electrophoresis system (SDS‐AGE). For separating plasma membrane AGP species of the same apparent molecular weight but with different net charge, a two‐dimensional electrophoresis system was used, utilizing isoelectric focusing in an immobilized pH gradient in the first dimension and SDS‐AGE in the second dimension. These methods enabled the separation of individual plasma membrane AGPs. In comparison, SDS‐PAGE methods left AGPs as unresolved high molecular‐weight smears. The methods described here may help to establish some basic features of AGPs, such as the number, organization, and protein and carbohydrate characteristics of plasma membrane AGPs, as well as the relationship between plasma membrane and extracellular AGPs.}},
  author       = {{Stöhr, Christine and Snogerup, Lars and Pennell, Roger and Kjellbom, Per}},
  issn         = {{1365-313X}},
  language     = {{eng}},
  number       = {{5}},
  pages        = {{943--948}},
  publisher    = {{Wiley-Blackwell}},
  series       = {{Plant Journal}},
  title        = {{An agarose gel electrophoresis method for the separation of arabinogalactan proteins.}},
  url          = {{http://dx.doi.org/10.1046/j.1365-313X.1996.10050943.x}},
  doi          = {{10.1046/j.1365-313X.1996.10050943.x}},
  volume       = {{10}},
  year         = {{1996}},
}