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Endotoxin increases both protein and fluid microvascular permeability in cat skeletal muscle

Holbeck, Staffan LU and Grände, Per-Olof LU (2003) In Critical Care Medicine 31(2). p.560-565
Abstract
Objective: To evaluate effects of lipopolysaccharide (endotoxin) on protein and fluid permeability in a whole organ skeletal muscle preparation. Design: Controlled, prospective laboratory study. Setting: University research laboratory. Subjects: Eleven adult male cats. Interventions: The study was performed on the autoperfused and denervated calf muscles of the cat hindlimb placed in a fluid-filled plethysmograph. The endotoxin-induced change in the osmotic reflection coefficient for albumin was used as a measure of alteration in protein permeability of the microvascular wall, and the simultaneous change in capillary filtration coefficient was used as a measure of alteration in fluid permeability. Endotoxin as a bolus infusion (1 mg/kg iv)... (More)
Objective: To evaluate effects of lipopolysaccharide (endotoxin) on protein and fluid permeability in a whole organ skeletal muscle preparation. Design: Controlled, prospective laboratory study. Setting: University research laboratory. Subjects: Eleven adult male cats. Interventions: The study was performed on the autoperfused and denervated calf muscles of the cat hindlimb placed in a fluid-filled plethysmograph. The endotoxin-induced change in the osmotic reflection coefficient for albumin was used as a measure of alteration in protein permeability of the microvascular wall, and the simultaneous change in capillary filtration coefficient was used as a measure of alteration in fluid permeability. Endotoxin as a bolus infusion (1 mg/kg iv) was given to six cats, and another five cats given only the vehicle (NaCl) were used as control. Measurements and Main Results: Arterial blood flow, arterial and venous blood pressures, total vascular resistance, and tissue volume changes were measured continuously. The ratio between the osmotic reflection coefficients for albumin on two occasions (before and about 1.5 hr after endotoxin infusion) was calculated from the Starling fluid equilibrium equation. This was performed by measurement of the maximum absorption rate from an iso-volumetric state by an intravenous bolus infusion of 20% human albumin (0.6 g/kg) and the capillary filtration coefficient. Albumin concentrations were measured before and after the albumin infusion to correct for effects of difference in plasma volume on the induced increase in colloid osmotic pressure. We found that the osmotic reflection coefficient for albumin was reduced by 30% (p < .05), and the capillary filtration coefficient was increased by 31% (p < .05) by endotoxin. No changes were seen in the vehicle experiments. Conclusion: Endotoxin causes a significant increase in both protein and fluid microvascular wall permeability. These effects may explain the marked leakage of plasma to the interstitium that is often seen in critically ill patients with sepsis and systemic inflammatory response syndrome. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
reflection coefficient, lipopolysaccharide, hypovolemia, fluid therapy, edema, coefficient, capillary filtration, critical illness, albumin, critical care, sepsis, systemic inflammatory response syndrome
in
Critical Care Medicine
volume
31
issue
2
pages
560 - 565
publisher
Lippincott Williams & Wilkins
external identifiers
  • pmid:12576966
  • wos:000180919700031
  • scopus:0037323911
ISSN
1530-0293
DOI
10.1097/01.CCM.0000048620.88344.70
language
English
LU publication?
yes
id
361fc701-34e1-4ac0-b9c0-3078df02f033 (old id 318732)
date added to LUP
2007-09-13 10:42:37
date last changed
2017-01-01 06:37:56
@article{361fc701-34e1-4ac0-b9c0-3078df02f033,
  abstract     = {Objective: To evaluate effects of lipopolysaccharide (endotoxin) on protein and fluid permeability in a whole organ skeletal muscle preparation. Design: Controlled, prospective laboratory study. Setting: University research laboratory. Subjects: Eleven adult male cats. Interventions: The study was performed on the autoperfused and denervated calf muscles of the cat hindlimb placed in a fluid-filled plethysmograph. The endotoxin-induced change in the osmotic reflection coefficient for albumin was used as a measure of alteration in protein permeability of the microvascular wall, and the simultaneous change in capillary filtration coefficient was used as a measure of alteration in fluid permeability. Endotoxin as a bolus infusion (1 mg/kg iv) was given to six cats, and another five cats given only the vehicle (NaCl) were used as control. Measurements and Main Results: Arterial blood flow, arterial and venous blood pressures, total vascular resistance, and tissue volume changes were measured continuously. The ratio between the osmotic reflection coefficients for albumin on two occasions (before and about 1.5 hr after endotoxin infusion) was calculated from the Starling fluid equilibrium equation. This was performed by measurement of the maximum absorption rate from an iso-volumetric state by an intravenous bolus infusion of 20% human albumin (0.6 g/kg) and the capillary filtration coefficient. Albumin concentrations were measured before and after the albumin infusion to correct for effects of difference in plasma volume on the induced increase in colloid osmotic pressure. We found that the osmotic reflection coefficient for albumin was reduced by 30% (p &lt; .05), and the capillary filtration coefficient was increased by 31% (p &lt; .05) by endotoxin. No changes were seen in the vehicle experiments. Conclusion: Endotoxin causes a significant increase in both protein and fluid microvascular wall permeability. These effects may explain the marked leakage of plasma to the interstitium that is often seen in critically ill patients with sepsis and systemic inflammatory response syndrome.},
  author       = {Holbeck, Staffan and Grände, Per-Olof},
  issn         = {1530-0293},
  keyword      = {reflection coefficient,lipopolysaccharide,hypovolemia,fluid therapy,edema,coefficient,capillary filtration,critical illness,albumin,critical care,sepsis,systemic inflammatory response syndrome},
  language     = {eng},
  number       = {2},
  pages        = {560--565},
  publisher    = {Lippincott Williams & Wilkins},
  series       = {Critical Care Medicine},
  title        = {Endotoxin increases both protein and fluid microvascular permeability in cat skeletal muscle},
  url          = {http://dx.doi.org/10.1097/01.CCM.0000048620.88344.70},
  volume       = {31},
  year         = {2003},
}