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Investigation of the mediated electron transfer mechanism of cellobiose dehydrogenase at cytochrome c-modified gold electrodes

Sarauli, David; Ludwig, Roland; Haltrich, Dietmar; Gorton, Lo LU and Lisdat, Fred (2012) In Bioelectrochemistry 87. p.9-14
Abstract
The present study reports on the comparison of direct and mediated electron transfer pathways in the interaction of the fungal enzyme cellobiose dehydrogenase (CDH) with the redox protein cytochrome c (cyt c) immobilised at a modified gold electrode surface. Two types of CDHs were chosen for this investigation: a basidiomycete (white rot) CDH from Trametes villosa and a recently discovered ascomycete from the thermophilic fungus Corynascus thermophilus. The choice was based on the pH-dependent interaction of these enzymes with cyt c in solution containing the substrate cellobiose (CB). Both enzymes show rather similar catalytic behaviour at lower pH, dominated by a direct electron exchange with the electrode. With increasing pH, however,... (More)
The present study reports on the comparison of direct and mediated electron transfer pathways in the interaction of the fungal enzyme cellobiose dehydrogenase (CDH) with the redox protein cytochrome c (cyt c) immobilised at a modified gold electrode surface. Two types of CDHs were chosen for this investigation: a basidiomycete (white rot) CDH from Trametes villosa and a recently discovered ascomycete from the thermophilic fungus Corynascus thermophilus. The choice was based on the pH-dependent interaction of these enzymes with cyt c in solution containing the substrate cellobiose (CB). Both enzymes show rather similar catalytic behaviour at lower pH, dominated by a direct electron exchange with the electrode. With increasing pH, however, also cyt c-mediated electron transfer becomes possible. The pH-dependent behaviour in the presence and in the absence of cyt c is analysed and the potential reaction mechanism for the two enzymes with a different pH-behaviour is discussed. (c) 2011 Published by Elsevier B.V. (Less)
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organization
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Contribution to journal
publication status
published
subject
keywords
Cellobiose dehydrogenase, Direct electron transfer, Mediated electron, transfer, Cytochrome c, Immobilised enzyme
in
Bioelectrochemistry
volume
87
pages
9 - 14
publisher
Elsevier
external identifiers
  • wos:000309033000003
  • scopus:84862857845
ISSN
1878-562X
DOI
10.1016/j.bioelechem.2011.07.003
language
English
LU publication?
yes
id
6a236a2e-330c-4dfa-a02c-9e47a27dc39f (old id 3187457)
date added to LUP
2012-12-06 12:26:17
date last changed
2017-09-24 03:10:47
@article{6a236a2e-330c-4dfa-a02c-9e47a27dc39f,
  abstract     = {The present study reports on the comparison of direct and mediated electron transfer pathways in the interaction of the fungal enzyme cellobiose dehydrogenase (CDH) with the redox protein cytochrome c (cyt c) immobilised at a modified gold electrode surface. Two types of CDHs were chosen for this investigation: a basidiomycete (white rot) CDH from Trametes villosa and a recently discovered ascomycete from the thermophilic fungus Corynascus thermophilus. The choice was based on the pH-dependent interaction of these enzymes with cyt c in solution containing the substrate cellobiose (CB). Both enzymes show rather similar catalytic behaviour at lower pH, dominated by a direct electron exchange with the electrode. With increasing pH, however, also cyt c-mediated electron transfer becomes possible. The pH-dependent behaviour in the presence and in the absence of cyt c is analysed and the potential reaction mechanism for the two enzymes with a different pH-behaviour is discussed. (c) 2011 Published by Elsevier B.V.},
  author       = {Sarauli, David and Ludwig, Roland and Haltrich, Dietmar and Gorton, Lo and Lisdat, Fred},
  issn         = {1878-562X},
  keyword      = {Cellobiose dehydrogenase,Direct electron transfer,Mediated electron,transfer,Cytochrome c,Immobilised enzyme},
  language     = {eng},
  pages        = {9--14},
  publisher    = {Elsevier},
  series       = {Bioelectrochemistry},
  title        = {Investigation of the mediated electron transfer mechanism of cellobiose dehydrogenase at cytochrome c-modified gold electrodes},
  url          = {http://dx.doi.org/10.1016/j.bioelechem.2011.07.003},
  volume       = {87},
  year         = {2012},
}