Effect of cholesterol on the lateral nanoscale dynamics of fluid membranes
(2012) In European Biophysics Journal 41(10). p.901-913- Abstract
- Inelastic neutron scattering was used to study the effect of 5 and 40 mol% cholesterol on the lateral nanoscale dynamics of phospholipid membranes. By measuring the excitation spectrum at several lateral q (||) values (up to q (||) = 3 (-1)), complete dispersion curves were determined of gel, fluid and liquid-ordered phase bilayers. The inclusion of cholesterol had a distinct effect on the collective dynamics of the bilayer's hydrocarbon chains; specifically, we observed a pronounced stiffening of the membranes on the nanometer length scale in both gel and fluid bilayers, even though they were experiencing a higher degree of molecular disorder. Also, for the first time we determined the nanoscale dynamics in the high-cholesterol... (More)
- Inelastic neutron scattering was used to study the effect of 5 and 40 mol% cholesterol on the lateral nanoscale dynamics of phospholipid membranes. By measuring the excitation spectrum at several lateral q (||) values (up to q (||) = 3 (-1)), complete dispersion curves were determined of gel, fluid and liquid-ordered phase bilayers. The inclusion of cholesterol had a distinct effect on the collective dynamics of the bilayer's hydrocarbon chains; specifically, we observed a pronounced stiffening of the membranes on the nanometer length scale in both gel and fluid bilayers, even though they were experiencing a higher degree of molecular disorder. Also, for the first time we determined the nanoscale dynamics in the high-cholesterol liquid-ordered phase of bilayers containing cholesterol. Namely, this phase appears to be "softer" than fluid bilayers, but better ordered than bilayers in the gel phase. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/3187484
- author
- Armstrong, Clare L. ; Barrett, Matthew A. ; Hiess, Arno LU ; Salditt, Tim ; Katsaras, John ; Shi, An-Chang and Rheinstaedter, Maikel C.
- organization
- publishing date
- 2012
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Lipid membrane, Cholesterol, Lateral membrane dynamics, Nanoscale, dynamics, Liquid-ordered phase, Inelastic neutron scattering, Dispersion, relation
- in
- European Biophysics Journal
- volume
- 41
- issue
- 10
- pages
- 901 - 913
- publisher
- Springer
- external identifiers
-
- wos:000309475700012
- scopus:84867571932
- pmid:22729214
- ISSN
- 0175-7571
- DOI
- 10.1007/s00249-012-0826-4
- language
- English
- LU publication?
- yes
- id
- 36bfff90-f9fd-4a21-9c26-5e155b1050a4 (old id 3187484)
- date added to LUP
- 2016-04-01 14:32:16
- date last changed
- 2022-03-06 19:51:26
@article{36bfff90-f9fd-4a21-9c26-5e155b1050a4,
abstract = {{Inelastic neutron scattering was used to study the effect of 5 and 40 mol% cholesterol on the lateral nanoscale dynamics of phospholipid membranes. By measuring the excitation spectrum at several lateral q (||) values (up to q (||) = 3 (-1)), complete dispersion curves were determined of gel, fluid and liquid-ordered phase bilayers. The inclusion of cholesterol had a distinct effect on the collective dynamics of the bilayer's hydrocarbon chains; specifically, we observed a pronounced stiffening of the membranes on the nanometer length scale in both gel and fluid bilayers, even though they were experiencing a higher degree of molecular disorder. Also, for the first time we determined the nanoscale dynamics in the high-cholesterol liquid-ordered phase of bilayers containing cholesterol. Namely, this phase appears to be "softer" than fluid bilayers, but better ordered than bilayers in the gel phase.}},
author = {{Armstrong, Clare L. and Barrett, Matthew A. and Hiess, Arno and Salditt, Tim and Katsaras, John and Shi, An-Chang and Rheinstaedter, Maikel C.}},
issn = {{0175-7571}},
keywords = {{Lipid membrane; Cholesterol; Lateral membrane dynamics; Nanoscale; dynamics; Liquid-ordered phase; Inelastic neutron scattering; Dispersion; relation}},
language = {{eng}},
number = {{10}},
pages = {{901--913}},
publisher = {{Springer}},
series = {{European Biophysics Journal}},
title = {{Effect of cholesterol on the lateral nanoscale dynamics of fluid membranes}},
url = {{http://dx.doi.org/10.1007/s00249-012-0826-4}},
doi = {{10.1007/s00249-012-0826-4}},
volume = {{41}},
year = {{2012}},
}