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Effect of cholesterol on the lateral nanoscale dynamics of fluid membranes

Armstrong, Clare L.; Barrett, Matthew A.; Hiess, Arno LU ; Salditt, Tim; Katsaras, John; Shi, An-Chang and Rheinstaedter, Maikel C. (2012) In European Biophysics Journal 41(10). p.901-913
Abstract
Inelastic neutron scattering was used to study the effect of 5 and 40 mol% cholesterol on the lateral nanoscale dynamics of phospholipid membranes. By measuring the excitation spectrum at several lateral q (||) values (up to q (||) = 3 (-1)), complete dispersion curves were determined of gel, fluid and liquid-ordered phase bilayers. The inclusion of cholesterol had a distinct effect on the collective dynamics of the bilayer's hydrocarbon chains; specifically, we observed a pronounced stiffening of the membranes on the nanometer length scale in both gel and fluid bilayers, even though they were experiencing a higher degree of molecular disorder. Also, for the first time we determined the nanoscale dynamics in the high-cholesterol... (More)
Inelastic neutron scattering was used to study the effect of 5 and 40 mol% cholesterol on the lateral nanoscale dynamics of phospholipid membranes. By measuring the excitation spectrum at several lateral q (||) values (up to q (||) = 3 (-1)), complete dispersion curves were determined of gel, fluid and liquid-ordered phase bilayers. The inclusion of cholesterol had a distinct effect on the collective dynamics of the bilayer's hydrocarbon chains; specifically, we observed a pronounced stiffening of the membranes on the nanometer length scale in both gel and fluid bilayers, even though they were experiencing a higher degree of molecular disorder. Also, for the first time we determined the nanoscale dynamics in the high-cholesterol liquid-ordered phase of bilayers containing cholesterol. Namely, this phase appears to be "softer" than fluid bilayers, but better ordered than bilayers in the gel phase. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Lipid membrane, Cholesterol, Lateral membrane dynamics, Nanoscale, dynamics, Liquid-ordered phase, Inelastic neutron scattering, Dispersion, relation
in
European Biophysics Journal
volume
41
issue
10
pages
901 - 913
publisher
Springer
external identifiers
  • wos:000309475700012
  • scopus:84867571932
ISSN
0175-7571
DOI
10.1007/s00249-012-0826-4
language
English
LU publication?
yes
id
36bfff90-f9fd-4a21-9c26-5e155b1050a4 (old id 3187484)
date added to LUP
2012-12-06 13:05:27
date last changed
2017-11-19 03:57:26
@article{36bfff90-f9fd-4a21-9c26-5e155b1050a4,
  abstract     = {Inelastic neutron scattering was used to study the effect of 5 and 40 mol% cholesterol on the lateral nanoscale dynamics of phospholipid membranes. By measuring the excitation spectrum at several lateral q (||) values (up to q (||) = 3 (-1)), complete dispersion curves were determined of gel, fluid and liquid-ordered phase bilayers. The inclusion of cholesterol had a distinct effect on the collective dynamics of the bilayer's hydrocarbon chains; specifically, we observed a pronounced stiffening of the membranes on the nanometer length scale in both gel and fluid bilayers, even though they were experiencing a higher degree of molecular disorder. Also, for the first time we determined the nanoscale dynamics in the high-cholesterol liquid-ordered phase of bilayers containing cholesterol. Namely, this phase appears to be "softer" than fluid bilayers, but better ordered than bilayers in the gel phase.},
  author       = {Armstrong, Clare L. and Barrett, Matthew A. and Hiess, Arno and Salditt, Tim and Katsaras, John and Shi, An-Chang and Rheinstaedter, Maikel C.},
  issn         = {0175-7571},
  keyword      = {Lipid membrane,Cholesterol,Lateral membrane dynamics,Nanoscale,dynamics,Liquid-ordered phase,Inelastic neutron scattering,Dispersion,relation},
  language     = {eng},
  number       = {10},
  pages        = {901--913},
  publisher    = {Springer},
  series       = {European Biophysics Journal},
  title        = {Effect of cholesterol on the lateral nanoscale dynamics of fluid membranes},
  url          = {http://dx.doi.org/10.1007/s00249-012-0826-4},
  volume       = {41},
  year         = {2012},
}