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Interaction of serum amyloid A with human cystatin Cuidentification of binding sites

Spodzieja, Marta; Szymanska, Aneta; Kolodziejczyk, Aleksandra; Pradzinska, Martyna; Maszota, Martyna; Stefanowicz, Piotr; Szewczuk, Zbigniew; Grubb, Anders LU and Czaplewska, Paulina (2012) In Journal of Molecular Recognition 25(10). p.513-524
Abstract
Serum amyloid A (SAA) is a multifunctional acute-phase protein whose natural role seems to be participation in many physiologic and pathological processes. Prolonged increased SAA level in a number of chronic inflammatory and neoplastic diseases gives rise to reactive systemic amyloid A amyloidosis, where the N-terminal 76-amino acid residue-long segment of SAA is deposited as amyloid fibrils. Recently, a specific interaction between SAA and the ubiquitous inhibitor of cysteine proteaseshuman cystatin C (hCC)has been described. Here, we report further evidence corroborating this interaction, and the identification of the SAA and hCC binding sites in the SAAhCC complex, using a combination of selective proteolytic excision and... (More)
Serum amyloid A (SAA) is a multifunctional acute-phase protein whose natural role seems to be participation in many physiologic and pathological processes. Prolonged increased SAA level in a number of chronic inflammatory and neoplastic diseases gives rise to reactive systemic amyloid A amyloidosis, where the N-terminal 76-amino acid residue-long segment of SAA is deposited as amyloid fibrils. Recently, a specific interaction between SAA and the ubiquitous inhibitor of cysteine proteaseshuman cystatin C (hCC)has been described. Here, we report further evidence corroborating this interaction, and the identification of the SAA and hCC binding sites in the SAAhCC complex, using a combination of selective proteolytic excision and high-resolution mass spectrometry. The shortest binding site in the SAA sequence was determined as SAA(86104), whereas the binding site in hCC sequence was identified as hCC(96102). Binding specificities of both interacting sequences were ascertained by affinity experiments (ELISA) and by registration of mass spectrum of SAAhCC complex. Copyright (c) 2012 John Wiley & Sons, Ltd. (Less)
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author
organization
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type
Contribution to journal
publication status
published
subject
keywords
serum amyloid A, human cystatin C, protein-protein interaction, mass, spectrometry
in
Journal of Molecular Recognition
volume
25
issue
10
pages
513 - 524
publisher
John Wiley & Sons
external identifiers
  • wos:000309067200004
  • scopus:84866426295
ISSN
1099-1352
DOI
10.1002/jmr.2220
language
English
LU publication?
yes
id
fb05286d-4a1d-452e-94a6-0264839ec990 (old id 3190037)
date added to LUP
2012-12-03 07:10:30
date last changed
2017-09-10 03:29:29
@article{fb05286d-4a1d-452e-94a6-0264839ec990,
  abstract     = {Serum amyloid A (SAA) is a multifunctional acute-phase protein whose natural role seems to be participation in many physiologic and pathological processes. Prolonged increased SAA level in a number of chronic inflammatory and neoplastic diseases gives rise to reactive systemic amyloid A amyloidosis, where the N-terminal 76-amino acid residue-long segment of SAA is deposited as amyloid fibrils. Recently, a specific interaction between SAA and the ubiquitous inhibitor of cysteine proteaseshuman cystatin C (hCC)has been described. Here, we report further evidence corroborating this interaction, and the identification of the SAA and hCC binding sites in the SAAhCC complex, using a combination of selective proteolytic excision and high-resolution mass spectrometry. The shortest binding site in the SAA sequence was determined as SAA(86104), whereas the binding site in hCC sequence was identified as hCC(96102). Binding specificities of both interacting sequences were ascertained by affinity experiments (ELISA) and by registration of mass spectrum of SAAhCC complex. Copyright (c) 2012 John Wiley & Sons, Ltd.},
  author       = {Spodzieja, Marta and Szymanska, Aneta and Kolodziejczyk, Aleksandra and Pradzinska, Martyna and Maszota, Martyna and Stefanowicz, Piotr and Szewczuk, Zbigniew and Grubb, Anders and Czaplewska, Paulina},
  issn         = {1099-1352},
  keyword      = {serum amyloid A,human cystatin C,protein-protein interaction,mass,spectrometry},
  language     = {eng},
  number       = {10},
  pages        = {513--524},
  publisher    = {John Wiley & Sons},
  series       = {Journal of Molecular Recognition},
  title        = {Interaction of serum amyloid A with human cystatin Cuidentification of binding sites},
  url          = {http://dx.doi.org/10.1002/jmr.2220},
  volume       = {25},
  year         = {2012},
}