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The effects of physical activity and estrogen treatment on rat fast and slow skeletal muscles following ovariectomy

Kadi, Fawzi; Karlsson, Camilla; Larsson, Britt LU ; Eriksson, Jennie; Larval, Maria; Billig, Håkan and Jonsdottir, Ingibjörg H. (2002) In Journal of Muscle Research and Cell Motility 23(4). p.335-339
Abstract
Decreased estrogen production is associated with changes in the skeletal, cardiovascular and muscular systems. At the level of skeletal muscles, it has been shown that a reduction in force production occurs at menopause but the underlying mechanisms are still unknown. The aim of the study was to investigate the effects of ovariectomy on myosin heavy chain (MyHC) composition. Additionally, we studied the effects of physical activity and the combined effects of physical activity and estrogen treatment on MyHC content in ovariectomised (OX) animals. Twenty-five rats were randomly assigned to five different groups: controls, runners, OX, ovariectomised runners and ovariectomised runners receiving estrogen. Exercise consisted of voluntary... (More)
Decreased estrogen production is associated with changes in the skeletal, cardiovascular and muscular systems. At the level of skeletal muscles, it has been shown that a reduction in force production occurs at menopause but the underlying mechanisms are still unknown. The aim of the study was to investigate the effects of ovariectomy on myosin heavy chain (MyHC) composition. Additionally, we studied the effects of physical activity and the combined effects of physical activity and estrogen treatment on MyHC content in ovariectomised (OX) animals. Twenty-five rats were randomly assigned to five different groups: controls, runners, OX, ovariectomised runners and ovariectomised runners receiving estrogen. Exercise consisted of voluntary running for 5 weeks. Two muscles were analysed: m. extensor digitorum longus, EDL, (fast muscle) and m. soleus (slow muscle). MyHC content was analysed on 8% gel electrophoresis. The level of running activity is reduced in OX animals and estrogen administration is associated with the normalisation of the level of physical activity. Ovariectomy induces a shift from fast to slow MyHC isoforms in both the soleus and EDL. When OX animals are allowed to run, alterations in MyHC isoforms are still observed in the EDL but not in the soleus. When physical activity is combined with estrogen treatment no alterations are observed in both muscles. In conclusion, this study shows that ovariectomy induces alterations in the contractile properties of skeletal muscles and that physical activity in combination with estrogen treatment are associated with the maintenance of slow and fast muscle characteristics. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Muscle Research and Cell Motility
volume
23
issue
4
pages
335 - 339
publisher
Springer
external identifiers
  • pmid:12630708
  • wos:000180454400010
  • scopus:0036991988
ISSN
0142-4319
DOI
10.1023/A:1022071114344
language
English
LU publication?
yes
id
3102e8b6-5710-4e43-83cc-920dbe1b576d (old id 319789)
date added to LUP
2007-10-31 15:55:31
date last changed
2017-08-06 04:23:46
@article{3102e8b6-5710-4e43-83cc-920dbe1b576d,
  abstract     = {Decreased estrogen production is associated with changes in the skeletal, cardiovascular and muscular systems. At the level of skeletal muscles, it has been shown that a reduction in force production occurs at menopause but the underlying mechanisms are still unknown. The aim of the study was to investigate the effects of ovariectomy on myosin heavy chain (MyHC) composition. Additionally, we studied the effects of physical activity and the combined effects of physical activity and estrogen treatment on MyHC content in ovariectomised (OX) animals. Twenty-five rats were randomly assigned to five different groups: controls, runners, OX, ovariectomised runners and ovariectomised runners receiving estrogen. Exercise consisted of voluntary running for 5 weeks. Two muscles were analysed: m. extensor digitorum longus, EDL, (fast muscle) and m. soleus (slow muscle). MyHC content was analysed on 8% gel electrophoresis. The level of running activity is reduced in OX animals and estrogen administration is associated with the normalisation of the level of physical activity. Ovariectomy induces a shift from fast to slow MyHC isoforms in both the soleus and EDL. When OX animals are allowed to run, alterations in MyHC isoforms are still observed in the EDL but not in the soleus. When physical activity is combined with estrogen treatment no alterations are observed in both muscles. In conclusion, this study shows that ovariectomy induces alterations in the contractile properties of skeletal muscles and that physical activity in combination with estrogen treatment are associated with the maintenance of slow and fast muscle characteristics.},
  author       = {Kadi, Fawzi and Karlsson, Camilla and Larsson, Britt and Eriksson, Jennie and Larval, Maria and Billig, Håkan and Jonsdottir, Ingibjörg H.},
  issn         = {0142-4319},
  language     = {eng},
  number       = {4},
  pages        = {335--339},
  publisher    = {Springer},
  series       = {Journal of Muscle Research and Cell Motility},
  title        = {The effects of physical activity and estrogen treatment on rat fast and slow skeletal muscles following ovariectomy},
  url          = {http://dx.doi.org/10.1023/A:1022071114344},
  volume       = {23},
  year         = {2002},
}