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Visualization of adult fish lens fiber cells

Kozłowski, Tomasz M. LU and Kröger, Ronald H.H. LU (2019) In Experimental Eye Research 181. p.1-4
Abstract

The crystalline lens of a vertebrate eye is a gradient-index lens and grows throughout life by addition of new lens fiber cells in the periphery. In fish, the growing ball-shaped lens maintains sophisticated optical properties throughout life by maintaining the distribution of refractive index relative to the increasing radius of the lens. During this process, the central fibers must increase refractive index by increasing the cytosolic concentration of crystallin proteins. However, only the youngest, most peripheral lens fiber cells have the ability to synthesize proteins. Unfortunately, the hardness of fish lenses makes investigation of the cellular anatomy impossible with traditional histological methods. We have developed a method... (More)

The crystalline lens of a vertebrate eye is a gradient-index lens and grows throughout life by addition of new lens fiber cells in the periphery. In fish, the growing ball-shaped lens maintains sophisticated optical properties throughout life by maintaining the distribution of refractive index relative to the increasing radius of the lens. During this process, the central fibers must increase refractive index by increasing the cytosolic concentration of crystallin proteins. However, only the youngest, most peripheral lens fiber cells have the ability to synthesize proteins. Unfortunately, the hardness of fish lenses makes investigation of the cellular anatomy impossible with traditional histological methods. We have developed a method for visualizing lens fiber cells across the diameter of the lens in adult fish. The method relies on sectioning embedded lenses with a high-speed power saw and observing the cut surface with a scanning electron microscope (SEM). The combination of SEM and image analysis allowed for precise tracking of the positions of individual cell fiber cells. As an application of the method, we present a cell thickness profile, i.e. the distribution of cells thicknesses and their relative positions along the lens's radius. Combined with detailed optical studies, which by mathematical reasons only are possible on ball-shaped lenses, our method can lead to new insights into the mechanism governing the functional and cellular development of vertebrate lenses.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Crystalline lens, Fiber cells, Fish, Lens anatomy, Optics
in
Experimental Eye Research
volume
181
pages
4 pages
publisher
Elsevier
external identifiers
  • scopus:85059649102
ISSN
0014-4835
DOI
10.1016/j.exer.2018.12.013
language
English
LU publication?
yes
id
319dea67-1e34-4a55-925e-7561b4fbfdde
date added to LUP
2019-01-16 14:46:53
date last changed
2019-02-20 11:43:13
@article{319dea67-1e34-4a55-925e-7561b4fbfdde,
  abstract     = {<p>The crystalline lens of a vertebrate eye is a gradient-index lens and grows throughout life by addition of new lens fiber cells in the periphery. In fish, the growing ball-shaped lens maintains sophisticated optical properties throughout life by maintaining the distribution of refractive index relative to the increasing radius of the lens. During this process, the central fibers must increase refractive index by increasing the cytosolic concentration of crystallin proteins. However, only the youngest, most peripheral lens fiber cells have the ability to synthesize proteins. Unfortunately, the hardness of fish lenses makes investigation of the cellular anatomy impossible with traditional histological methods. We have developed a method for visualizing lens fiber cells across the diameter of the lens in adult fish. The method relies on sectioning embedded lenses with a high-speed power saw and observing the cut surface with a scanning electron microscope (SEM). The combination of SEM and image analysis allowed for precise tracking of the positions of individual cell fiber cells. As an application of the method, we present a cell thickness profile, i.e. the distribution of cells thicknesses and their relative positions along the lens's radius. Combined with detailed optical studies, which by mathematical reasons only are possible on ball-shaped lenses, our method can lead to new insights into the mechanism governing the functional and cellular development of vertebrate lenses.</p>},
  author       = {Kozłowski, Tomasz M. and Kröger, Ronald H.H.},
  issn         = {0014-4835},
  keyword      = {Crystalline lens,Fiber cells,Fish,Lens anatomy,Optics},
  language     = {eng},
  month        = {04},
  pages        = {1--4},
  publisher    = {Elsevier},
  series       = {Experimental Eye Research},
  title        = {Visualization of adult fish lens fiber cells},
  url          = {http://dx.doi.org/10.1016/j.exer.2018.12.013},
  volume       = {181},
  year         = {2019},
}