Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

Comparative immunogenicity of decellularized wild type and alpha 1,3 galactosyltransferase knockout pig lungs

Gasek, N ; Dearborn, J ; Rolandsson Enes, Sara LU orcid ; Pouliot, R ; Louie, J ; Phillips, Z ; Wrenn, S ; Uhl, FE ; Riveron, A and Bianchi, J , et al. (2021) In Biomaterials 276.
Abstract
Decellularized pig lungs recellularized with human lung cells offer a novel approach for organ transplantation. However, the potential immunogenicity of decellularized pig lungs following exposure to human tissues has not been assessed. We found that exposure of native lungs from wildtype and transgenic pigs lacking alpha (1,3)-galactosyltransferase (α-gal KO) to sera from normal healthy human volunteers demonstrated similar robust IgM and IgG immunoreactivity, comparably decreased in decellularized lungs. Similar results were observed with sera from patients who had previously undergone transcutaneous porcine aortic valve replacement (TAVR) or from patients with increased circulating anti-α-gal IgE antibodies (α-gal syndrome). Depleting... (More)
Decellularized pig lungs recellularized with human lung cells offer a novel approach for organ transplantation. However, the potential immunogenicity of decellularized pig lungs following exposure to human tissues has not been assessed. We found that exposure of native lungs from wildtype and transgenic pigs lacking alpha (1,3)-galactosyltransferase (α-gal KO) to sera from normal healthy human volunteers demonstrated similar robust IgM and IgG immunoreactivity, comparably decreased in decellularized lungs. Similar results were observed with sera from patients who had previously undergone transcutaneous porcine aortic valve replacement (TAVR) or from patients with increased circulating anti-α-gal IgE antibodies (α-gal syndrome). Depleting anti-α-gal antibodies from the sera demonstrated both specificity of α-gal immunoreactivity and also residual immunoreactivity similar between wildtype and α-gal KO pig lungs. Exposure of human monocytes and macrophages to native wildtype lungs demonstrated greater induction of M2 phenotype than native α-gal KO pig lungs, which was less marked with decellularized lungs of either type. Overall, these results demonstrate that native wildtype and α-gal KO pig lungs provoke similar immune responses that are comparably decreased following decellularization. This provides a further platform for potential use of decellularized pig lungs in tissue engineering approaches and subsequent transplantation schemes but no obvious overall immunologic advantage of utilizing lungs obtained from α-gal KO pigs. (Less)
Please use this url to cite or link to this publication:
author
; ; ; ; ; ; ; ; and , et al. (More)
; ; ; ; ; ; ; ; ; ; ; ; ; ; ; and (Less)
publishing date
type
Contribution to journal
publication status
published
subject
in
Biomaterials
volume
276
article number
121029
publisher
Elsevier
external identifiers
  • scopus:85111148279
ISSN
1878-5905
DOI
10.1016/j.biomaterials.2021.121029
language
English
LU publication?
no
id
31a95751-6504-4270-9c19-38032786a48b
date added to LUP
2022-12-16 09:52:43
date last changed
2023-12-19 23:02:54
@article{31a95751-6504-4270-9c19-38032786a48b,
  abstract     = {{Decellularized pig lungs recellularized with human lung cells offer a novel approach for organ transplantation. However, the potential immunogenicity of decellularized pig lungs following exposure to human tissues has not been assessed. We found that exposure of native lungs from wildtype and transgenic pigs lacking alpha (1,3)-galactosyltransferase (α-gal KO) to sera from normal healthy human volunteers demonstrated similar robust IgM and IgG immunoreactivity, comparably decreased in decellularized lungs. Similar results were observed with sera from patients who had previously undergone transcutaneous porcine aortic valve replacement (TAVR) or from patients with increased circulating anti-α-gal IgE antibodies (α-gal syndrome). Depleting anti-α-gal antibodies from the sera demonstrated both specificity of α-gal immunoreactivity and also residual immunoreactivity similar between wildtype and α-gal KO pig lungs. Exposure of human monocytes and macrophages to native wildtype lungs demonstrated greater induction of M2 phenotype than native α-gal KO pig lungs, which was less marked with decellularized lungs of either type. Overall, these results demonstrate that native wildtype and α-gal KO pig lungs provoke similar immune responses that are comparably decreased following decellularization. This provides a further platform for potential use of decellularized pig lungs in tissue engineering approaches and subsequent transplantation schemes but no obvious overall immunologic advantage of utilizing lungs obtained from α-gal KO pigs.}},
  author       = {{Gasek, N and Dearborn, J and Rolandsson Enes, Sara and Pouliot, R and Louie, J and Phillips, Z and Wrenn, S and Uhl, FE and Riveron, A and Bianchi, J and Commins, SP and Delance, N and Taatjes, DJ and Boyson, JE and Guthrie, K and Petersen, TH and J Weiss, D}},
  issn         = {{1878-5905}},
  language     = {{eng}},
  publisher    = {{Elsevier}},
  series       = {{Biomaterials}},
  title        = {{Comparative immunogenicity of decellularized wild type and alpha 1,3 galactosyltransferase knockout pig lungs}},
  url          = {{http://dx.doi.org/10.1016/j.biomaterials.2021.121029}},
  doi          = {{10.1016/j.biomaterials.2021.121029}},
  volume       = {{276}},
  year         = {{2021}},
}