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Structural characterization of fibrous synthetic hydrogels using fluorescence microscopy

Vandaele, Johannes ; Louis, Boris LU ; Liu, Kaizheng ; Camacho, Rafael ; Kouwer, Paul H.J. and Rocha, Susana (2020) In Soft Matter 16(17). p.4210-4219
Abstract

The structural features of the matrix surrounding the cells play a crucial role in regulating their behavior. Here, we used fluorescence microscopy and customized analysis algorithms to characterize the architecture of fibrous hydrogel networks. As a model system, we investigated a new class of synthetic biomimetic material, hydrogels prepared from polyisocyanides. Our results show that these synthetic gels present a highly heterogeneous fibrous network, with pores reaching a few micrometers in diameter. By encapsulating HeLa cells in different hydrogels, we show that a more porous structure is linked to a higher proliferation rate. The approach described here, for the characterization of the network of fibrous hydrogels, can be easily... (More)

The structural features of the matrix surrounding the cells play a crucial role in regulating their behavior. Here, we used fluorescence microscopy and customized analysis algorithms to characterize the architecture of fibrous hydrogel networks. As a model system, we investigated a new class of synthetic biomimetic material, hydrogels prepared from polyisocyanides. Our results show that these synthetic gels present a highly heterogeneous fibrous network, with pores reaching a few micrometers in diameter. By encapsulating HeLa cells in different hydrogels, we show that a more porous structure is linked to a higher proliferation rate. The approach described here, for the characterization of the network of fibrous hydrogels, can be easily applied to other polymer-based materials and provide new insights into the influence of structural features in cell behavior. This knowledge is crucial to develop the next generation of biomimetic materials for 3D cell models and tissue engineering applications.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Soft Matter
volume
16
issue
17
pages
10 pages
publisher
Royal Society of Chemistry
external identifiers
  • scopus:85084379560
  • pmid:32292943
ISSN
1744-683X
DOI
10.1039/c9sm01828j
language
English
LU publication?
yes
id
31e86734-246d-4249-be13-97c34d70c63a
date added to LUP
2020-06-09 09:41:42
date last changed
2020-08-02 07:00:59
@article{31e86734-246d-4249-be13-97c34d70c63a,
  abstract     = {<p>The structural features of the matrix surrounding the cells play a crucial role in regulating their behavior. Here, we used fluorescence microscopy and customized analysis algorithms to characterize the architecture of fibrous hydrogel networks. As a model system, we investigated a new class of synthetic biomimetic material, hydrogels prepared from polyisocyanides. Our results show that these synthetic gels present a highly heterogeneous fibrous network, with pores reaching a few micrometers in diameter. By encapsulating HeLa cells in different hydrogels, we show that a more porous structure is linked to a higher proliferation rate. The approach described here, for the characterization of the network of fibrous hydrogels, can be easily applied to other polymer-based materials and provide new insights into the influence of structural features in cell behavior. This knowledge is crucial to develop the next generation of biomimetic materials for 3D cell models and tissue engineering applications.</p>},
  author       = {Vandaele, Johannes and Louis, Boris and Liu, Kaizheng and Camacho, Rafael and Kouwer, Paul H.J. and Rocha, Susana},
  issn         = {1744-683X},
  language     = {eng},
  month        = {05},
  number       = {17},
  pages        = {4210--4219},
  publisher    = {Royal Society of Chemistry},
  series       = {Soft Matter},
  title        = {Structural characterization of fibrous synthetic hydrogels using fluorescence microscopy},
  url          = {http://dx.doi.org/10.1039/c9sm01828j},
  doi          = {10.1039/c9sm01828j},
  volume       = {16},
  year         = {2020},
}