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Composition and microarchitecture of human trabecular bone change with age and differ between anatomical locations

Turunen, Mikael J.; Prantner, Viktoria; Jurvelin, Jukka S.; Kroger, Heikki and Isaksson, Hanna LU (2013) In Bone 54(1). p.118-125
Abstract
The microarchitecture of trabecular bone adapts to its mechanical loading environment according to Wolff's law and alters with age. Trabecular bone is a metabolically active tissue, thus, its molecular composition and microarchitecture may vary between anatomical locations as a result of the local mechanical loading environment. No comprehensive comparison of composition and microarchitecture of trabecular bone in different anatomical locations has been conducted. Therefore, the objective of this study was to compare the molecular composition and microarchitecture, evaluated with Fourier transform infrared (FTIR) microspectroscopy and micro-computed tomography (mu CT), respectively, in the femoral neck, greater trochanter and calcaneus of... (More)
The microarchitecture of trabecular bone adapts to its mechanical loading environment according to Wolff's law and alters with age. Trabecular bone is a metabolically active tissue, thus, its molecular composition and microarchitecture may vary between anatomical locations as a result of the local mechanical loading environment. No comprehensive comparison of composition and microarchitecture of trabecular bone in different anatomical locations has been conducted. Therefore, the objective of this study was to compare the molecular composition and microarchitecture, evaluated with Fourier transform infrared (FTIR) microspectroscopy and micro-computed tomography (mu CT), respectively, in the femoral neck, greater trochanter and calcaneus of human cadavers. Specimens were harvested from 20 male human cadavers (aged 17-82 years) with no known metabolic bone diseases. Significant differences were found in composition and microarchitecture of trabecular bone between the anatomical locations. Compositional differences were primarily observed between the calcaneus and the proximal femur sites. Mineralization was higher in the greater trochanter than in the calcaneus (+2%, p<0.05) and crystallinity was lowest in the calcaneus (-24%, p<0.05 as compared to the femoral neck). Variation in the composition of trabecular bone within different parts of the proximal femur was only minor. Collagen maturity was significantly lower in greater trochanter than in femoral neck (-8%, p<0.01) and calcaneus (-5%, p<0.05). The greater trochanter possessed a less dense trabecular bone microarchitecture compared to femoral neck or calcaneus. Age related changes were mainly found in the greater trochanter. Significant correlations were found between the composition and microarchitecture of trabecular bone in the greater trochanter and calcaneus, indicating that both composition and microarchitecture alter similarly. This study provides new information about composition and microarchitecture of trabecular bone in different anatomical locations and their alterations with age with respect to the anatomical loading environments. (C) 2013 Elsevier Inc. All rights reserved. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Trabecular bone, Composition, Microarchitecture, Fourier transform, infrared (FTIR) microspectroscopy, Micro-computed tomography (mu CT)
in
Bone
volume
54
issue
1
pages
118 - 125
publisher
Elsevier
external identifiers
  • wos:000316839800016
  • scopus:84874354025
ISSN
1873-2763
DOI
10.1016/j.bone.2013.01.045
language
English
LU publication?
yes
id
31eb7b44-5e1a-40a6-aa11-ee6c6fd71452 (old id 3853602)
date added to LUP
2013-06-20 08:43:48
date last changed
2019-03-12 02:17:04
@article{31eb7b44-5e1a-40a6-aa11-ee6c6fd71452,
  abstract     = {The microarchitecture of trabecular bone adapts to its mechanical loading environment according to Wolff's law and alters with age. Trabecular bone is a metabolically active tissue, thus, its molecular composition and microarchitecture may vary between anatomical locations as a result of the local mechanical loading environment. No comprehensive comparison of composition and microarchitecture of trabecular bone in different anatomical locations has been conducted. Therefore, the objective of this study was to compare the molecular composition and microarchitecture, evaluated with Fourier transform infrared (FTIR) microspectroscopy and micro-computed tomography (mu CT), respectively, in the femoral neck, greater trochanter and calcaneus of human cadavers. Specimens were harvested from 20 male human cadavers (aged 17-82 years) with no known metabolic bone diseases. Significant differences were found in composition and microarchitecture of trabecular bone between the anatomical locations. Compositional differences were primarily observed between the calcaneus and the proximal femur sites. Mineralization was higher in the greater trochanter than in the calcaneus (+2%, p&lt;0.05) and crystallinity was lowest in the calcaneus (-24%, p&lt;0.05 as compared to the femoral neck). Variation in the composition of trabecular bone within different parts of the proximal femur was only minor. Collagen maturity was significantly lower in greater trochanter than in femoral neck (-8%, p&lt;0.01) and calcaneus (-5%, p&lt;0.05). The greater trochanter possessed a less dense trabecular bone microarchitecture compared to femoral neck or calcaneus. Age related changes were mainly found in the greater trochanter. Significant correlations were found between the composition and microarchitecture of trabecular bone in the greater trochanter and calcaneus, indicating that both composition and microarchitecture alter similarly. This study provides new information about composition and microarchitecture of trabecular bone in different anatomical locations and their alterations with age with respect to the anatomical loading environments. (C) 2013 Elsevier Inc. All rights reserved.},
  author       = {Turunen, Mikael J. and Prantner, Viktoria and Jurvelin, Jukka S. and Kroger, Heikki and Isaksson, Hanna},
  issn         = {1873-2763},
  keyword      = {Trabecular bone,Composition,Microarchitecture,Fourier transform,infrared (FTIR) microspectroscopy,Micro-computed tomography (mu CT)},
  language     = {eng},
  number       = {1},
  pages        = {118--125},
  publisher    = {Elsevier},
  series       = {Bone},
  title        = {Composition and microarchitecture of human trabecular bone change with age and differ between anatomical locations},
  url          = {http://dx.doi.org/10.1016/j.bone.2013.01.045},
  volume       = {54},
  year         = {2013},
}