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Nanoclay-induced bacterial flocculation for infection confinement

Malekkhaiat Häffner, Sara ; Nyström, Lina ; Strömstedt, Adam A. ; Li, Li ; van der Plas, Mariena J.A. LU and Malmsten, Martin LU (2020) In Journal of Colloid and Interface Science 562. p.71-80
Abstract

Effects of size and charge of anionic nanoclays on their interactions with bacteria-mimicking lipid membranes, bacterial lipopolysaccharide (LPS), and Gram-negative bacteria were investigated using ellipsometry, dynamic light scattering, ζ-potential measurements, and confocal microscopy combined with Live/Dead staining. Based on particle size and charge density, three different anionic hectorite nanoclays were employed, and investigated in the presence and absence of the net cationic human antimicrobial peptide LL-37 (LLGDFFRKSKEKIGKEFKRIVQRIKDFLRNLVPRTES). In the absence of this peptide, the nanoclays were found not to bind to similarly anionic bacteria-mimicking model phospholipid membranes, nor to destabilize these. Similarly, while... (More)

Effects of size and charge of anionic nanoclays on their interactions with bacteria-mimicking lipid membranes, bacterial lipopolysaccharide (LPS), and Gram-negative bacteria were investigated using ellipsometry, dynamic light scattering, ζ-potential measurements, and confocal microscopy combined with Live/Dead staining. Based on particle size and charge density, three different anionic hectorite nanoclays were employed, and investigated in the presence and absence of the net cationic human antimicrobial peptide LL-37 (LLGDFFRKSKEKIGKEFKRIVQRIKDFLRNLVPRTES). In the absence of this peptide, the nanoclays were found not to bind to similarly anionic bacteria-mimicking model phospholipid membranes, nor to destabilize these. Similarly, while all nanoclays induced aggregation of Escherichia coli bacteria, the flocculated bacteria remained alive after aggregation. In contrast, LL-37 alone, i.e. in the absence of nanoclay particles, displays antimicrobial properties through membrane lysis, but does not cause bacterial aggregation in the concentration range investigated. After loading the nanoclays with LL-37, potent bacterial aggregation combined with bacterial membrane lysis was observed for all nanoclay sizes and charge densities. Demonstrating the potential of these combined systems for confinement of infection, LPS-induced NF-κB activation in human monocytes was found to be strongly suppressed after nanoclay-mediated aggregation, with a wide tolerance for nanoparticle size and charge density.

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author
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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Antimicrobial, Bacteria flocculation, Infection confinement, Membrane
in
Journal of Colloid and Interface Science
volume
562
pages
10 pages
publisher
Elsevier
external identifiers
  • scopus:85076261572
  • pmid:31837621
ISSN
0021-9797
DOI
10.1016/j.jcis.2019.11.110
language
English
LU publication?
yes
id
31ff0592-1830-4cc1-8cc7-4eeeff26c19d
date added to LUP
2021-01-12 14:43:34
date last changed
2024-06-13 04:55:09
@article{31ff0592-1830-4cc1-8cc7-4eeeff26c19d,
  abstract     = {{<p>Effects of size and charge of anionic nanoclays on their interactions with bacteria-mimicking lipid membranes, bacterial lipopolysaccharide (LPS), and Gram-negative bacteria were investigated using ellipsometry, dynamic light scattering, ζ-potential measurements, and confocal microscopy combined with Live/Dead staining. Based on particle size and charge density, three different anionic hectorite nanoclays were employed, and investigated in the presence and absence of the net cationic human antimicrobial peptide LL-37 (LLGDFFRKSKEKIGKEFKRIVQRIKDFLRNLVPRTES). In the absence of this peptide, the nanoclays were found not to bind to similarly anionic bacteria-mimicking model phospholipid membranes, nor to destabilize these. Similarly, while all nanoclays induced aggregation of Escherichia coli bacteria, the flocculated bacteria remained alive after aggregation. In contrast, LL-37 alone, i.e. in the absence of nanoclay particles, displays antimicrobial properties through membrane lysis, but does not cause bacterial aggregation in the concentration range investigated. After loading the nanoclays with LL-37, potent bacterial aggregation combined with bacterial membrane lysis was observed for all nanoclay sizes and charge densities. Demonstrating the potential of these combined systems for confinement of infection, LPS-induced NF-κB activation in human monocytes was found to be strongly suppressed after nanoclay-mediated aggregation, with a wide tolerance for nanoparticle size and charge density.</p>}},
  author       = {{Malekkhaiat Häffner, Sara and Nyström, Lina and Strömstedt, Adam A. and Li, Li and van der Plas, Mariena J.A. and Malmsten, Martin}},
  issn         = {{0021-9797}},
  keywords     = {{Antimicrobial; Bacteria flocculation; Infection confinement; Membrane}},
  language     = {{eng}},
  pages        = {{71--80}},
  publisher    = {{Elsevier}},
  series       = {{Journal of Colloid and Interface Science}},
  title        = {{Nanoclay-induced bacterial flocculation for infection confinement}},
  url          = {{http://dx.doi.org/10.1016/j.jcis.2019.11.110}},
  doi          = {{10.1016/j.jcis.2019.11.110}},
  volume       = {{562}},
  year         = {{2020}},
}