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All IncP-1 plasmid subgroups, including the novel epsilon subgroup, are prevalent in the influent of a Danish wastewater treatment plant

Bahl, M. I.; Burmolle, M.; Meisner, Annelein LU ; Hansen, L. H. and Sorensen, S. J. (2009) In Plasmid 62(2). p.134-139
Abstract
The presence and diversity of IncP-1 plasmids in the influent of a Danish wastewater treatment plant was studied by PCR amplification of the trfA gene in community DNA followed by sequencing. Three sets of PCR primers were designed to amplify a 281 bp fragment of trfA from all currently sequenced IncP-1 plasmids. A neighbor-joining tree, based on a multiple alignment of 72 obtained sequences together with homologous sequences of previously published IncP-1 plasmids, revealed that all established subgroups of IncP-1 plasmids, alpha, beta, gamma and delta, were present in the wastewater treatment plant influent. Also sequences representing the recently described fifth subgroup, the epsilon subgroup, were detected in the wastewater. Thus,... (More)
The presence and diversity of IncP-1 plasmids in the influent of a Danish wastewater treatment plant was studied by PCR amplification of the trfA gene in community DNA followed by sequencing. Three sets of PCR primers were designed to amplify a 281 bp fragment of trfA from all currently sequenced IncP-1 plasmids. A neighbor-joining tree, based on a multiple alignment of 72 obtained sequences together with homologous sequences of previously published IncP-1 plasmids, revealed that all established subgroups of IncP-1 plasmids, alpha, beta, gamma and delta, were present in the wastewater treatment plant influent. Also sequences representing the recently described fifth subgroup, the epsilon subgroup, were detected in the wastewater. Thus, these results confirm the presence of at least five phylogenetically distinct subgroups of IncP-1 plasmids and represent the first time that sequences associated with plasmids of all of these five subgroups have been detected in a single setting. Additionally, the results confirm that wastewater constitutes a reservoir for the conjugative IncP-1 plasmids, which often harbor multiple antibiotic resistance genes. (c) 2009 Elsevier Inc. All rights reserved. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
provides evidence, host-range plasmids, antibiotic-resistance plasmids, Phylogeny, Wastewater, PCR, Plasmid, IncP-1, alginate lyase, sequence, soil, transposon, bacteria, genes, degradation
in
Plasmid
volume
62
issue
2
pages
134 - 139
publisher
Academic Press
external identifiers
  • scopus:67650710749
ISSN
0147-619X
DOI
10.1016/j.plasmid.2009.05.004
language
English
LU publication?
no
id
b4816c3a-a7ad-4005-b1d1-37125dbf13cf (old id 3216556)
date added to LUP
2012-11-29 16:01:10
date last changed
2017-08-27 03:57:31
@article{b4816c3a-a7ad-4005-b1d1-37125dbf13cf,
  abstract     = {The presence and diversity of IncP-1 plasmids in the influent of a Danish wastewater treatment plant was studied by PCR amplification of the trfA gene in community DNA followed by sequencing. Three sets of PCR primers were designed to amplify a 281 bp fragment of trfA from all currently sequenced IncP-1 plasmids. A neighbor-joining tree, based on a multiple alignment of 72 obtained sequences together with homologous sequences of previously published IncP-1 plasmids, revealed that all established subgroups of IncP-1 plasmids, alpha, beta, gamma and delta, were present in the wastewater treatment plant influent. Also sequences representing the recently described fifth subgroup, the epsilon subgroup, were detected in the wastewater. Thus, these results confirm the presence of at least five phylogenetically distinct subgroups of IncP-1 plasmids and represent the first time that sequences associated with plasmids of all of these five subgroups have been detected in a single setting. Additionally, the results confirm that wastewater constitutes a reservoir for the conjugative IncP-1 plasmids, which often harbor multiple antibiotic resistance genes. (c) 2009 Elsevier Inc. All rights reserved.},
  author       = {Bahl, M. I. and Burmolle, M. and Meisner, Annelein and Hansen, L. H. and Sorensen, S. J.},
  issn         = {0147-619X},
  keyword      = {provides evidence,host-range plasmids,antibiotic-resistance plasmids,Phylogeny,Wastewater,PCR,Plasmid,IncP-1,alginate lyase,sequence,soil,transposon,bacteria,genes,degradation},
  language     = {eng},
  number       = {2},
  pages        = {134--139},
  publisher    = {Academic Press},
  series       = {Plasmid},
  title        = {All IncP-1 plasmid subgroups, including the novel epsilon subgroup, are prevalent in the influent of a Danish wastewater treatment plant},
  url          = {http://dx.doi.org/10.1016/j.plasmid.2009.05.004},
  volume       = {62},
  year         = {2009},
}