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Functional importance of individual rRNA 2'-O-ribose methylations revealed by high-resolution phenotyping

Esguerra, Jonathan LU ; Warringer, J. and Blomberg, A. (2008) In RNA 14(4). p.56-649
Abstract
Ribosomal RNAs contain numerous modifications at specific nucleotides. Despite their evolutionary conservation, the functional role of individual 2'-O-ribose methylations in rRNA is not known. A distinct family of small nucleolar RNAs, box C/D snoRNAs, guides the methylating complex to specific rRNA sites. Using a high-resolution phenotyping approach, we characterized 20 box C/D snoRNA gene deletions for altered growth dynamics under a wide array of environmental perturbations, encompassing intraribosomal antibiotics, inhibitors of specific cellular features, as well as general stressors. Ribosome-specific antibiotics generated phenotypes indicating different and long-ranging structural effects of rRNA methylations on the ribosome. For all... (More)
Ribosomal RNAs contain numerous modifications at specific nucleotides. Despite their evolutionary conservation, the functional role of individual 2'-O-ribose methylations in rRNA is not known. A distinct family of small nucleolar RNAs, box C/D snoRNAs, guides the methylating complex to specific rRNA sites. Using a high-resolution phenotyping approach, we characterized 20 box C/D snoRNA gene deletions for altered growth dynamics under a wide array of environmental perturbations, encompassing intraribosomal antibiotics, inhibitors of specific cellular features, as well as general stressors. Ribosome-specific antibiotics generated phenotypes indicating different and long-ranging structural effects of rRNA methylations on the ribosome. For all studied box C/D snoRNA mutants we uncovered phenotypes to extraribosomal growth inhibitors, most frequently reflected in alteration in growth lag (adaptation time). A number of strains were highly pleiotropic and displayed a great number of sensitive phenotypes, e.g., deletion mutants of snR70 and snR71, which both have clear human homologues, and deletion mutants of snR65 and snR68. Our data indicate that individual rRNA ribose methylations can play either distinct or general roles in the workings of the ribosome. (Less)
Please use this url to cite or link to this publication:
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organization
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Contribution to journal
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published
subject
keywords
Ribosomal/*chemistry/genetics/*metabolism, Fungal/chemistry/genetics/metabolism, RNA, Phenotype, Nucleic Acid Conformation/drug effects, Methylation, Anti-Bacterial Agents/pharmacology, Fungal, Genes, Ribosomes/drug effects/metabolism, Saccharomyces cerevisiae/genetics/metabolism, Sequence Deletion, Ribose/*chemistry, Small Nucleolar/chemistry/genetics/metabolism
in
RNA
volume
14
issue
4
pages
56 - 649
publisher
Cold Spring Harbor Laboratory Press
external identifiers
  • scopus:41649115487
ISSN
1355-8382
DOI
10.1261/rna.845808
language
English
LU publication?
no
id
5d88669c-25fd-4e4b-a7a3-6f3b092aac0b (old id 3217782)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/18256246
http://www.ncbi.nlm.nih.gov/pubmed/18256246
date added to LUP
2012-11-30 15:11:38
date last changed
2017-10-29 04:31:47
@article{5d88669c-25fd-4e4b-a7a3-6f3b092aac0b,
  abstract     = {Ribosomal RNAs contain numerous modifications at specific nucleotides. Despite their evolutionary conservation, the functional role of individual 2'-O-ribose methylations in rRNA is not known. A distinct family of small nucleolar RNAs, box C/D snoRNAs, guides the methylating complex to specific rRNA sites. Using a high-resolution phenotyping approach, we characterized 20 box C/D snoRNA gene deletions for altered growth dynamics under a wide array of environmental perturbations, encompassing intraribosomal antibiotics, inhibitors of specific cellular features, as well as general stressors. Ribosome-specific antibiotics generated phenotypes indicating different and long-ranging structural effects of rRNA methylations on the ribosome. For all studied box C/D snoRNA mutants we uncovered phenotypes to extraribosomal growth inhibitors, most frequently reflected in alteration in growth lag (adaptation time). A number of strains were highly pleiotropic and displayed a great number of sensitive phenotypes, e.g., deletion mutants of snR70 and snR71, which both have clear human homologues, and deletion mutants of snR65 and snR68. Our data indicate that individual rRNA ribose methylations can play either distinct or general roles in the workings of the ribosome.},
  author       = {Esguerra, Jonathan and Warringer, J. and Blomberg, A.},
  issn         = {1355-8382},
  keyword      = {Ribosomal/*chemistry/genetics/*metabolism,Fungal/chemistry/genetics/metabolism,RNA,Phenotype,Nucleic Acid Conformation/drug effects,Methylation,Anti-Bacterial Agents/pharmacology,Fungal,Genes,Ribosomes/drug effects/metabolism,Saccharomyces cerevisiae/genetics/metabolism,Sequence Deletion,Ribose/*chemistry,Small Nucleolar/chemistry/genetics/metabolism},
  language     = {eng},
  number       = {4},
  pages        = {56--649},
  publisher    = {Cold Spring Harbor Laboratory Press},
  series       = {RNA},
  title        = {Functional importance of individual rRNA 2'-O-ribose methylations revealed by high-resolution phenotyping},
  url          = {http://dx.doi.org/10.1261/rna.845808},
  volume       = {14},
  year         = {2008},
}