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Neurite outgrowth and synaptophysin expression of postnatal CNS neurons on GaP nanowire arrays in long-term retinal cell culture.

Offranc-Piret, Gaelle LU ; Perez, Maria Thereza LU and Prinz, Christelle LU (2013) In Biomaterials 34(4). p.875-887
Abstract
We have established long-term cultures of postnatal retinal cells on arrays of gallium phosphide nanowires of different geometries. Rod and cone photoreceptors, ganglion cells and bipolar cells survived on the substrates for at least 18 days in vitro. Glial cells were also observed, but these did not overgrow the neuronal population. On nanowires, neurons extended numerous long and branched neurites that expressed the synaptic vesicle marker synaptophysin. The longest nanowires (4 μm long) allowed a greater attachment and neurite elongation and our analysis suggests that the length of the nanowire per se and/or the adsorption of biomolecules on the nanowires may have been important factors regulating the observed cell behavior. The study... (More)
We have established long-term cultures of postnatal retinal cells on arrays of gallium phosphide nanowires of different geometries. Rod and cone photoreceptors, ganglion cells and bipolar cells survived on the substrates for at least 18 days in vitro. Glial cells were also observed, but these did not overgrow the neuronal population. On nanowires, neurons extended numerous long and branched neurites that expressed the synaptic vesicle marker synaptophysin. The longest nanowires (4 μm long) allowed a greater attachment and neurite elongation and our analysis suggests that the length of the nanowire per se and/or the adsorption of biomolecules on the nanowires may have been important factors regulating the observed cell behavior. The study thus shows that CNS neurons are amenable to gallium phosphide nanowires, probably as they create conditions that more closely resemble those encountered in the in vivo environment. These findings suggest that gallium phosphide nanowires may be considered as a material of interest when improving existing or designing the next generation of implantable devices. The features of gallium phosphide nanowires can be precisely controlled, making them suitable for this purpose. (Less)
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organization
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type
Contribution to journal
publication status
published
subject
in
Biomaterials
volume
34
issue
4
pages
875 - 887
publisher
Elsevier
external identifiers
  • wos:000313155800004
  • pmid:23131535
  • scopus:84870317528
ISSN
1878-5905
DOI
10.1016/j.biomaterials.2012.10.042
language
English
LU publication?
yes
id
4bb64847-8c85-47e7-9870-0309eb8e6151 (old id 3219202)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/23131535?dopt=Abstract
date added to LUP
2012-12-03 13:09:36
date last changed
2018-04-22 04:07:24
@article{4bb64847-8c85-47e7-9870-0309eb8e6151,
  abstract     = {We have established long-term cultures of postnatal retinal cells on arrays of gallium phosphide nanowires of different geometries. Rod and cone photoreceptors, ganglion cells and bipolar cells survived on the substrates for at least 18 days in vitro. Glial cells were also observed, but these did not overgrow the neuronal population. On nanowires, neurons extended numerous long and branched neurites that expressed the synaptic vesicle marker synaptophysin. The longest nanowires (4 μm long) allowed a greater attachment and neurite elongation and our analysis suggests that the length of the nanowire per se and/or the adsorption of biomolecules on the nanowires may have been important factors regulating the observed cell behavior. The study thus shows that CNS neurons are amenable to gallium phosphide nanowires, probably as they create conditions that more closely resemble those encountered in the in vivo environment. These findings suggest that gallium phosphide nanowires may be considered as a material of interest when improving existing or designing the next generation of implantable devices. The features of gallium phosphide nanowires can be precisely controlled, making them suitable for this purpose.},
  author       = {Offranc-Piret, Gaelle and Perez, Maria Thereza and Prinz, Christelle},
  issn         = {1878-5905},
  language     = {eng},
  number       = {4},
  pages        = {875--887},
  publisher    = {Elsevier},
  series       = {Biomaterials},
  title        = {Neurite outgrowth and synaptophysin expression of postnatal CNS neurons on GaP nanowire arrays in long-term retinal cell culture.},
  url          = {http://dx.doi.org/10.1016/j.biomaterials.2012.10.042},
  volume       = {34},
  year         = {2013},
}