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Characterisation of a plasma membrane-associated phospholipase A(2) activity increased in response to cold acclimation

Gustavsson, Maria LU and Sommarin, Marianne LU (2002) In Journal of Plant Physiology 159(11). p.1219-1227
Abstract
We here demonstrate the presence of a plasma membrane-associated phospholipase A(2) (EC 3.1.1.4; PLA(2)) activity in spinach (Spinacia oleracea) leaves. The pH profile of the spinach plasma membrane PLA(2) activity revealed two peaks, one at pH 4.4 and one at pH 5.5. The activity at pH 5.5 had an absolute requirement of Ca2+, with full enzyme activity at 10mumol/L Ca2+. The Ca2+-dependent PLA(2) activity was both heat sensitive and stimulated by diacylglycerol, whereas ATP completely inhibited the activity. Thus, the spinach plasma membrane contains a Ca2+-dependent PLA(2) activity, which has not previously been characterised in plants. Cold acclimation of spinach resulted in a 2.2-fold higher plasma membrane PLA(2) activity whereas the... (More)
We here demonstrate the presence of a plasma membrane-associated phospholipase A(2) (EC 3.1.1.4; PLA(2)) activity in spinach (Spinacia oleracea) leaves. The pH profile of the spinach plasma membrane PLA(2) activity revealed two peaks, one at pH 4.4 and one at pH 5.5. The activity at pH 5.5 had an absolute requirement of Ca2+, with full enzyme activity at 10mumol/L Ca2+. The Ca2+-dependent PLA(2) activity was both heat sensitive and stimulated by diacylglycerol, whereas ATP completely inhibited the activity. Thus, the spinach plasma membrane contains a Ca2+-dependent PLA(2) activity, which has not previously been characterised in plants. Cold acclimation of spinach resulted in a 2.2-fold higher plasma membrane PLA(2) activity whereas the plasma membrane phospholipase D activity remained unaffected. Taken together, our data suggest a role of PLA(2) in cold acclimation in plants. (Less)
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author
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type
Contribution to journal
publication status
published
subject
keywords
Spinacia oleracea, plasma membrane, phospholipase A(2), Ca2+ dependency, cold acclimation
in
Journal of Plant Physiology
volume
159
issue
11
pages
1219 - 1227
publisher
Gustav Fischer Verlag
external identifiers
  • wos:000179581200008
  • scopus:0036857669
ISSN
0176-1617
DOI
10.1078/0176-1617-00724
language
English
LU publication?
yes
id
07918c6e-8078-41d8-a6a3-092ecd0ccc7b (old id 322338)
date added to LUP
2016-04-01 15:22:52
date last changed
2020-01-12 18:26:51
@article{07918c6e-8078-41d8-a6a3-092ecd0ccc7b,
  abstract     = {We here demonstrate the presence of a plasma membrane-associated phospholipase A(2) (EC 3.1.1.4; PLA(2)) activity in spinach (Spinacia oleracea) leaves. The pH profile of the spinach plasma membrane PLA(2) activity revealed two peaks, one at pH 4.4 and one at pH 5.5. The activity at pH 5.5 had an absolute requirement of Ca2+, with full enzyme activity at 10mumol/L Ca2+. The Ca2+-dependent PLA(2) activity was both heat sensitive and stimulated by diacylglycerol, whereas ATP completely inhibited the activity. Thus, the spinach plasma membrane contains a Ca2+-dependent PLA(2) activity, which has not previously been characterised in plants. Cold acclimation of spinach resulted in a 2.2-fold higher plasma membrane PLA(2) activity whereas the plasma membrane phospholipase D activity remained unaffected. Taken together, our data suggest a role of PLA(2) in cold acclimation in plants.},
  author       = {Gustavsson, Maria and Sommarin, Marianne},
  issn         = {0176-1617},
  language     = {eng},
  number       = {11},
  pages        = {1219--1227},
  publisher    = {Gustav Fischer Verlag},
  series       = {Journal of Plant Physiology},
  title        = {Characterisation of a plasma membrane-associated phospholipase A(2) activity increased in response to cold acclimation},
  url          = {http://dx.doi.org/10.1078/0176-1617-00724},
  doi          = {10.1078/0176-1617-00724},
  volume       = {159},
  year         = {2002},
}