Compaction and decompaction of DNA in the presence of catanionic amphiphile mixtures
(2002) In The Journal of Physical Chemistry Part B 106(48). p.12608-12612- Abstract
- The dissociation of DNA-cationic surfactant complexes with the addition of a negative amphiphile was studied by fluorescence microscopy (FM). The unfolding of DNA molecules previously compacted with cationic surfactant was shown to be dependent on the anionic surfactant chain length; lower amounts of a longer-chain surfactant were needed to release DNA into solution. However, we observed no dependence on the hydrophobicity of the compacting agent. The structures of the aggregates formed by the two surfactants, after the interaction with DNA, were imaged by cryogenic transmission electron microscopy (cryo-TEM). We found that it is possible to predict the structure of the aggregates the surfactants will form, such as vesicle phases, from the... (More)
- The dissociation of DNA-cationic surfactant complexes with the addition of a negative amphiphile was studied by fluorescence microscopy (FM). The unfolding of DNA molecules previously compacted with cationic surfactant was shown to be dependent on the anionic surfactant chain length; lower amounts of a longer-chain surfactant were needed to release DNA into solution. However, we observed no dependence on the hydrophobicity of the compacting agent. The structures of the aggregates formed by the two surfactants, after the interaction with DNA, were imaged by cryogenic transmission electron microscopy (cryo-TEM). We found that it is possible to predict the structure of the aggregates the surfactants will form, such as vesicle phases, from the behavior of mixed surfactant systems, which brings new insight to this subject and several interesting opportunities for applications. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/322351
- author
- Dias, Rita LU ; Lindman, Björn LU and Miguel, MG
- organization
- publishing date
- 2002
- type
- Contribution to journal
- publication status
- published
- subject
- in
- The Journal of Physical Chemistry Part B
- volume
- 106
- issue
- 48
- pages
- 12608 - 12612
- publisher
- The American Chemical Society (ACS)
- external identifiers
-
- wos:000179618200034
- scopus:0037028193
- ISSN
- 1520-5207
- DOI
- 10.1021/jp020392r
- language
- English
- LU publication?
- yes
- id
- 2502763b-672b-44dd-8347-6a8d2fdc30f0 (old id 322351)
- date added to LUP
- 2016-04-01 15:24:34
- date last changed
- 2022-01-28 05:10:44
@article{2502763b-672b-44dd-8347-6a8d2fdc30f0,
abstract = {{The dissociation of DNA-cationic surfactant complexes with the addition of a negative amphiphile was studied by fluorescence microscopy (FM). The unfolding of DNA molecules previously compacted with cationic surfactant was shown to be dependent on the anionic surfactant chain length; lower amounts of a longer-chain surfactant were needed to release DNA into solution. However, we observed no dependence on the hydrophobicity of the compacting agent. The structures of the aggregates formed by the two surfactants, after the interaction with DNA, were imaged by cryogenic transmission electron microscopy (cryo-TEM). We found that it is possible to predict the structure of the aggregates the surfactants will form, such as vesicle phases, from the behavior of mixed surfactant systems, which brings new insight to this subject and several interesting opportunities for applications.}},
author = {{Dias, Rita and Lindman, Björn and Miguel, MG}},
issn = {{1520-5207}},
language = {{eng}},
number = {{48}},
pages = {{12608--12612}},
publisher = {{The American Chemical Society (ACS)}},
series = {{The Journal of Physical Chemistry Part B}},
title = {{Compaction and decompaction of DNA in the presence of catanionic amphiphile mixtures}},
url = {{http://dx.doi.org/10.1021/jp020392r}},
doi = {{10.1021/jp020392r}},
volume = {{106}},
year = {{2002}},
}