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Compaction and decompaction of DNA in the presence of catanionic amphiphile mixtures

Dias, Rita LU ; Lindman, Björn LU and Miguel, MG (2002) In The Journal of Physical Chemistry Part B 106(48). p.12608-12612
Abstract
The dissociation of DNA-cationic surfactant complexes with the addition of a negative amphiphile was studied by fluorescence microscopy (FM). The unfolding of DNA molecules previously compacted with cationic surfactant was shown to be dependent on the anionic surfactant chain length; lower amounts of a longer-chain surfactant were needed to release DNA into solution. However, we observed no dependence on the hydrophobicity of the compacting agent. The structures of the aggregates formed by the two surfactants, after the interaction with DNA, were imaged by cryogenic transmission electron microscopy (cryo-TEM). We found that it is possible to predict the structure of the aggregates the surfactants will form, such as vesicle phases, from the... (More)
The dissociation of DNA-cationic surfactant complexes with the addition of a negative amphiphile was studied by fluorescence microscopy (FM). The unfolding of DNA molecules previously compacted with cationic surfactant was shown to be dependent on the anionic surfactant chain length; lower amounts of a longer-chain surfactant were needed to release DNA into solution. However, we observed no dependence on the hydrophobicity of the compacting agent. The structures of the aggregates formed by the two surfactants, after the interaction with DNA, were imaged by cryogenic transmission electron microscopy (cryo-TEM). We found that it is possible to predict the structure of the aggregates the surfactants will form, such as vesicle phases, from the behavior of mixed surfactant systems, which brings new insight to this subject and several interesting opportunities for applications. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
The Journal of Physical Chemistry Part B
volume
106
issue
48
pages
12608 - 12612
publisher
The American Chemical Society
external identifiers
  • wos:000179618200034
  • scopus:0037028193
ISSN
1520-5207
DOI
10.1021/jp020392r
language
English
LU publication?
yes
id
2502763b-672b-44dd-8347-6a8d2fdc30f0 (old id 322351)
date added to LUP
2007-08-16 09:10:58
date last changed
2017-12-10 04:25:12
@article{2502763b-672b-44dd-8347-6a8d2fdc30f0,
  abstract     = {The dissociation of DNA-cationic surfactant complexes with the addition of a negative amphiphile was studied by fluorescence microscopy (FM). The unfolding of DNA molecules previously compacted with cationic surfactant was shown to be dependent on the anionic surfactant chain length; lower amounts of a longer-chain surfactant were needed to release DNA into solution. However, we observed no dependence on the hydrophobicity of the compacting agent. The structures of the aggregates formed by the two surfactants, after the interaction with DNA, were imaged by cryogenic transmission electron microscopy (cryo-TEM). We found that it is possible to predict the structure of the aggregates the surfactants will form, such as vesicle phases, from the behavior of mixed surfactant systems, which brings new insight to this subject and several interesting opportunities for applications.},
  author       = {Dias, Rita and Lindman, Björn and Miguel, MG},
  issn         = {1520-5207},
  language     = {eng},
  number       = {48},
  pages        = {12608--12612},
  publisher    = {The American Chemical Society},
  series       = {The Journal of Physical Chemistry Part B},
  title        = {Compaction and decompaction of DNA in the presence of catanionic amphiphile mixtures},
  url          = {http://dx.doi.org/10.1021/jp020392r},
  volume       = {106},
  year         = {2002},
}