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Development of monoclonal antibodies against creatine kinase MB2

Leickt, L; Grubb, Anders LU and Ohlson, S (2002) In Scandinavian Journal of Clinical & Laboratory Investigation 62(6). p.423-429
Abstract
An increase of creatine kinase MB (CKMB) in serum has long been used as a marker for acute myocardial infarction (AMI). It is important with an early diagnosis and since the amount of the CKMB2 isoform rises above reference levels much earlier than the total creatine kinase amount, quantification of CKMB isoforms could be a feasible alternative for early analysis. The two CKMB isoforms differ by only one C-terminal lysine residue, which makes it difficult to separate one from the other. To overcome this problem, monoclonal antibodies were produced using unique peptides as antigen in hybridoma technology. Two peptides with 16 and 15 amino acids corresponding to the C-terminal end of the M-subunits of CKMB2 and CKMB1 respectively, were... (More)
An increase of creatine kinase MB (CKMB) in serum has long been used as a marker for acute myocardial infarction (AMI). It is important with an early diagnosis and since the amount of the CKMB2 isoform rises above reference levels much earlier than the total creatine kinase amount, quantification of CKMB isoforms could be a feasible alternative for early analysis. The two CKMB isoforms differ by only one C-terminal lysine residue, which makes it difficult to separate one from the other. To overcome this problem, monoclonal antibodies were produced using unique peptides as antigen in hybridoma technology. Two peptides with 16 and 15 amino acids corresponding to the C-terminal end of the M-subunits of CKMB2 and CKMB1 respectively, were conjugated to keyhole limpet hemocyanin and used as antigens. Sixteen different monoclonal antibodies to these peptides were obtained and characterized. Their specificity was analyzed by immunoassay and 10 of the antibodies showed cross-reactive binding to creatine kinase. Surface plasmon resonance based biosensor analysis was used to determine affinity and kinetics towards the peptides and the epitopes of four of the antibodies were studied by means of phage display. Some of these antibodies have binding properties that might qualify them for use in the establishment of procedures allowing early diagnosis of AMI. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
acute myocardial infarction, CKMB2, peptide antigen
in
Scandinavian Journal of Clinical & Laboratory Investigation
volume
62
issue
6
pages
423 - 429
publisher
Informa Healthcare
external identifiers
  • wos:000179228200003
  • pmid:12469897
  • scopus:0036428763
ISSN
1502-7686
DOI
10.1080/00365510260389976
language
English
LU publication?
yes
id
5b4bdcb1-ed2e-4c2c-a55e-69c41b800501 (old id 322989)
date added to LUP
2007-10-31 10:39:30
date last changed
2017-01-01 07:25:40
@article{5b4bdcb1-ed2e-4c2c-a55e-69c41b800501,
  abstract     = {An increase of creatine kinase MB (CKMB) in serum has long been used as a marker for acute myocardial infarction (AMI). It is important with an early diagnosis and since the amount of the CKMB2 isoform rises above reference levels much earlier than the total creatine kinase amount, quantification of CKMB isoforms could be a feasible alternative for early analysis. The two CKMB isoforms differ by only one C-terminal lysine residue, which makes it difficult to separate one from the other. To overcome this problem, monoclonal antibodies were produced using unique peptides as antigen in hybridoma technology. Two peptides with 16 and 15 amino acids corresponding to the C-terminal end of the M-subunits of CKMB2 and CKMB1 respectively, were conjugated to keyhole limpet hemocyanin and used as antigens. Sixteen different monoclonal antibodies to these peptides were obtained and characterized. Their specificity was analyzed by immunoassay and 10 of the antibodies showed cross-reactive binding to creatine kinase. Surface plasmon resonance based biosensor analysis was used to determine affinity and kinetics towards the peptides and the epitopes of four of the antibodies were studied by means of phage display. Some of these antibodies have binding properties that might qualify them for use in the establishment of procedures allowing early diagnosis of AMI.},
  author       = {Leickt, L and Grubb, Anders and Ohlson, S},
  issn         = {1502-7686},
  keyword      = {acute myocardial infarction,CKMB2,peptide antigen},
  language     = {eng},
  number       = {6},
  pages        = {423--429},
  publisher    = {Informa Healthcare},
  series       = {Scandinavian Journal of Clinical & Laboratory Investigation},
  title        = {Development of monoclonal antibodies against creatine kinase MB2},
  url          = {http://dx.doi.org/10.1080/00365510260389976},
  volume       = {62},
  year         = {2002},
}