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Crystal structure of Na+, K(+)-ATPase in the Na(+)-bound state

Nyblom, Maria ; Poulsen, Hanne ; Gourdon, Pontus LU ; Reinhard, Linda ; Andersson, Magnus ; Lindahl, Erik ; Fedosova, Natalya and Nissen, Poul (2013) In Science (New York, N.Y.) 342(6154). p.7-123
Abstract

The Na(+), K(+)-adenosine triphosphatase (ATPase) maintains the electrochemical gradients of Na(+) and K(+) across the plasma membrane--a prerequisite for electrical excitability and secondary transport. Hitherto, structural information has been limited to K(+)-bound or ouabain-blocked forms. We present the crystal structure of a Na(+)-bound Na(+), K(+)-ATPase as determined at 4.3 Å resolution. Compared with the K(+)-bound form, large conformational changes are observed in the α subunit whereas the β and γ subunit structures are maintained. The locations of the three Na(+) sites are indicated with the unique site III at the recently suggested IIIb, as further supported by electrophysiological studies on leak currents. Extracellular... (More)

The Na(+), K(+)-adenosine triphosphatase (ATPase) maintains the electrochemical gradients of Na(+) and K(+) across the plasma membrane--a prerequisite for electrical excitability and secondary transport. Hitherto, structural information has been limited to K(+)-bound or ouabain-blocked forms. We present the crystal structure of a Na(+)-bound Na(+), K(+)-ATPase as determined at 4.3 Å resolution. Compared with the K(+)-bound form, large conformational changes are observed in the α subunit whereas the β and γ subunit structures are maintained. The locations of the three Na(+) sites are indicated with the unique site III at the recently suggested IIIb, as further supported by electrophysiological studies on leak currents. Extracellular release of the third Na(+) from IIIb through IIIa, followed by exchange of Na(+) for K(+) at sites I and II, is suggested.

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author
; ; ; ; ; ; and
publishing date
type
Contribution to journal
publication status
published
keywords
Animals, Cell Membrane, Crystallography, X-Ray, Models, Molecular, Mutation, Protein Binding, Protein Structure, Secondary, Protein Structure, Tertiary, Sodium, Sodium-Potassium-Exchanging ATPase, Swine, Journal Article, Research Support, Non-U.S. Gov't
in
Science (New York, N.Y.)
volume
342
issue
6154
pages
5 pages
publisher
American Association for the Advancement of Science (AAAS)
external identifiers
  • scopus:84885653302
  • pmid:24051246
ISSN
1095-9203
DOI
10.1126/science.1243352
language
English
LU publication?
no
id
322ab871-9dc9-470f-bfde-4e2eb5c27eac
date added to LUP
2017-04-29 15:29:32
date last changed
2024-04-28 11:25:02
@article{322ab871-9dc9-470f-bfde-4e2eb5c27eac,
  abstract     = {{<p>The Na(+), K(+)-adenosine triphosphatase (ATPase) maintains the electrochemical gradients of Na(+) and K(+) across the plasma membrane--a prerequisite for electrical excitability and secondary transport. Hitherto, structural information has been limited to K(+)-bound or ouabain-blocked forms. We present the crystal structure of a Na(+)-bound Na(+), K(+)-ATPase as determined at 4.3 Å resolution. Compared with the K(+)-bound form, large conformational changes are observed in the α subunit whereas the β and γ subunit structures are maintained. The locations of the three Na(+) sites are indicated with the unique site III at the recently suggested IIIb, as further supported by electrophysiological studies on leak currents. Extracellular release of the third Na(+) from IIIb through IIIa, followed by exchange of Na(+) for K(+) at sites I and II, is suggested.</p>}},
  author       = {{Nyblom, Maria and Poulsen, Hanne and Gourdon, Pontus and Reinhard, Linda and Andersson, Magnus and Lindahl, Erik and Fedosova, Natalya and Nissen, Poul}},
  issn         = {{1095-9203}},
  keywords     = {{Animals; Cell Membrane; Crystallography, X-Ray; Models, Molecular; Mutation; Protein Binding; Protein Structure, Secondary; Protein Structure, Tertiary; Sodium; Sodium-Potassium-Exchanging ATPase; Swine; Journal Article; Research Support, Non-U.S. Gov't}},
  language     = {{eng}},
  month        = {{10}},
  number       = {{6154}},
  pages        = {{7--123}},
  publisher    = {{American Association for the Advancement of Science (AAAS)}},
  series       = {{Science (New York, N.Y.)}},
  title        = {{Crystal structure of Na+, K(+)-ATPase in the Na(+)-bound state}},
  url          = {{http://dx.doi.org/10.1126/science.1243352}},
  doi          = {{10.1126/science.1243352}},
  volume       = {{342}},
  year         = {{2013}},
}