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Crystal structure of Na+, K(+)-ATPase in the Na(+)-bound state

Nyblom, Maria; Poulsen, Hanne; Gourdon, Pontus LU ; Reinhard, Linda; Andersson, Magnus; Lindahl, Erik; Fedosova, Natalya and Nissen, Poul (2013) In Science (New York, N.Y.) 342(6154). p.7-123
Abstract

The Na(+), K(+)-adenosine triphosphatase (ATPase) maintains the electrochemical gradients of Na(+) and K(+) across the plasma membrane--a prerequisite for electrical excitability and secondary transport. Hitherto, structural information has been limited to K(+)-bound or ouabain-blocked forms. We present the crystal structure of a Na(+)-bound Na(+), K(+)-ATPase as determined at 4.3 Å resolution. Compared with the K(+)-bound form, large conformational changes are observed in the α subunit whereas the β and γ subunit structures are maintained. The locations of the three Na(+) sites are indicated with the unique site III at the recently suggested IIIb, as further supported by electrophysiological studies on leak currents. Extracellular... (More)

The Na(+), K(+)-adenosine triphosphatase (ATPase) maintains the electrochemical gradients of Na(+) and K(+) across the plasma membrane--a prerequisite for electrical excitability and secondary transport. Hitherto, structural information has been limited to K(+)-bound or ouabain-blocked forms. We present the crystal structure of a Na(+)-bound Na(+), K(+)-ATPase as determined at 4.3 Å resolution. Compared with the K(+)-bound form, large conformational changes are observed in the α subunit whereas the β and γ subunit structures are maintained. The locations of the three Na(+) sites are indicated with the unique site III at the recently suggested IIIb, as further supported by electrophysiological studies on leak currents. Extracellular release of the third Na(+) from IIIb through IIIa, followed by exchange of Na(+) for K(+) at sites I and II, is suggested.

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Contribution to journal
publication status
published
keywords
Animals, Cell Membrane, Crystallography, X-Ray, Models, Molecular, Mutation, Protein Binding, Protein Structure, Secondary, Protein Structure, Tertiary, Sodium, Sodium-Potassium-Exchanging ATPase, Swine, Journal Article, Research Support, Non-U.S. Gov't
in
Science (New York, N.Y.)
volume
342
issue
6154
pages
5 pages
publisher
The American Association for the Advancement of Science
external identifiers
  • scopus:84885653302
ISSN
1095-9203
DOI
10.1126/science.1243352
language
English
LU publication?
no
id
322ab871-9dc9-470f-bfde-4e2eb5c27eac
date added to LUP
2017-04-29 15:29:32
date last changed
2018-08-12 04:35:41
@article{322ab871-9dc9-470f-bfde-4e2eb5c27eac,
  abstract     = {<p>The Na(+), K(+)-adenosine triphosphatase (ATPase) maintains the electrochemical gradients of Na(+) and K(+) across the plasma membrane--a prerequisite for electrical excitability and secondary transport. Hitherto, structural information has been limited to K(+)-bound or ouabain-blocked forms. We present the crystal structure of a Na(+)-bound Na(+), K(+)-ATPase as determined at 4.3 Å resolution. Compared with the K(+)-bound form, large conformational changes are observed in the α subunit whereas the β and γ subunit structures are maintained. The locations of the three Na(+) sites are indicated with the unique site III at the recently suggested IIIb, as further supported by electrophysiological studies on leak currents. Extracellular release of the third Na(+) from IIIb through IIIa, followed by exchange of Na(+) for K(+) at sites I and II, is suggested.</p>},
  author       = {Nyblom, Maria and Poulsen, Hanne and Gourdon, Pontus and Reinhard, Linda and Andersson, Magnus and Lindahl, Erik and Fedosova, Natalya and Nissen, Poul},
  issn         = {1095-9203},
  keyword      = {Animals,Cell Membrane,Crystallography, X-Ray,Models, Molecular,Mutation,Protein Binding,Protein Structure, Secondary,Protein Structure, Tertiary,Sodium,Sodium-Potassium-Exchanging ATPase,Swine,Journal Article,Research Support, Non-U.S. Gov't},
  language     = {eng},
  month        = {10},
  number       = {6154},
  pages        = {7--123},
  publisher    = {The American Association for the Advancement of Science},
  series       = {Science (New York, N.Y.)},
  title        = {Crystal structure of Na+, K(+)-ATPase in the Na(+)-bound state},
  url          = {http://dx.doi.org/10.1126/science.1243352},
  volume       = {342},
  year         = {2013},
}