A 125I-protein A-binding assay detecting antibodies to cell surface antigens - Evidence for the presence of specific antibodies against leukemia-associated antigens in human leukemias
Fäldt, R. LU and Ankerst, J. LU
(1983)
In Cancer Immunology Immunotherapy
15(2).
p.69-77
- Abstract
A 125I-protein A-binding assay detecting antibodies to cell surface antigens on human blood cells was developed and evaluated using sera from multitransfused nonleukemic patients sensitized against HLA antigens. The binding assay was found to be reproducible and more sensitive than conventional HLA testing. Seven patients with acute myelogenous leukemia and two patients with acute lymphoblastic leukemia successfully treated by chemotherapy were then investigated. Sera from seven of the patients studied in partial or complete remission demonstrated significant binding to autochthonous leukemic cells obtained from bone marrow or peripheral blood. In two cases sera taken during the leukemic stage demonstrated the most pronounced... (More)
A 125I-protein A-binding assay detecting antibodies to cell surface antigens on human blood cells was developed and evaluated using sera from multitransfused nonleukemic patients sensitized against HLA antigens. The binding assay was found to be reproducible and more sensitive than conventional HLA testing. Seven patients with acute myelogenous leukemia and two patients with acute lymphoblastic leukemia successfully treated by chemotherapy were then investigated. Sera from seven of the patients studied in partial or complete remission demonstrated significant binding to autochthonous leukemic cells obtained from bone marrow or peripheral blood. In two cases sera taken during the leukemic stage demonstrated the most pronounced binding to the patients' own leukemic cells. Sera from four patients with demonstrable significant binding to autochthonous leukemic cells failed to bind to autochthonous remission cells when both types of target cells were tested in parallel. Differences in serum concentrations of IgG, IgA, and IgM were not the cause of the demonstrated increased binding of leukemic sera to autochthonous target cells. We propose that the 125I-protein A-binding assay presented in this paper detects antibodies reacting selectively with acute leukemia cells.
(Less)
- author
- Fäldt, R.
LU
and Ankerst, J.
LU
- organization
- publishing date
- 1983
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Cancer Immunology Immunotherapy
- volume
- 15
- issue
- 2
- pages
- 9 pages
- publisher
- Springer
- external identifiers
-
- pmid:6223693
- scopus:0020643871
- ISSN
- 0340-7004
- DOI
- 10.1007/BF00199693
- language
- English
- LU publication?
- yes
- id
- 322e093c-78a5-4a75-a110-e809ce4b1177
- date added to LUP
- 2021-01-21 15:14:18
- date last changed
- 2024-01-03 05:35:46
@article{322e093c-78a5-4a75-a110-e809ce4b1177,
abstract = {{<p>A <sup>125</sup>I-protein A-binding assay detecting antibodies to cell surface antigens on human blood cells was developed and evaluated using sera from multitransfused nonleukemic patients sensitized against HLA antigens. The binding assay was found to be reproducible and more sensitive than conventional HLA testing. Seven patients with acute myelogenous leukemia and two patients with acute lymphoblastic leukemia successfully treated by chemotherapy were then investigated. Sera from seven of the patients studied in partial or complete remission demonstrated significant binding to autochthonous leukemic cells obtained from bone marrow or peripheral blood. In two cases sera taken during the leukemic stage demonstrated the most pronounced binding to the patients' own leukemic cells. Sera from four patients with demonstrable significant binding to autochthonous leukemic cells failed to bind to autochthonous remission cells when both types of target cells were tested in parallel. Differences in serum concentrations of IgG, IgA, and IgM were not the cause of the demonstrated increased binding of leukemic sera to autochthonous target cells. We propose that the <sup>125</sup>I-protein A-binding assay presented in this paper detects antibodies reacting selectively with acute leukemia cells.</p>}},
author = {{Fäldt, R. and Ankerst, J.}},
issn = {{0340-7004}},
language = {{eng}},
number = {{2}},
pages = {{69--77}},
publisher = {{Springer}},
series = {{Cancer Immunology Immunotherapy}},
title = {{A 125I-protein A-binding assay detecting antibodies
to cell surface antigens - Evidence for the presence of specific antibodies
against leukemia-associated antigens in human leukemias}},
url = {{http://dx.doi.org/10.1007/BF00199693}},
doi = {{10.1007/BF00199693}},
volume = {{15}},
year = {{1983}},
}