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Cyclin E and P27 protein content in human renal cell carcinoma: Clinical outcome and associations with cyclin D

Hedberg, Y; Davoodi, E; Ljungberg, B; Roos, G and Landberg, Göran LU (2002) In International Journal of Cancer 102(6). p.601-607
Abstract
Aberrations in the GI-S transition have been observed in several malignancies, suggesting that cell cycle defects are linked to the activation of oncogenes and inactivation of suppressor genes involved in the transformation process. The frequency of GI/S aberrations in human renal cell carcinoma (RCC) has not been fully clarified. We have therefore analyzed the cyclin E content, using Western blotting, in 79 RCC and 12 corresponding kidney cortex tissues as well as the fraction of p27-positive cells in 73 RCCs, using immunohistochemistry. Most of the tumors (65%) exhibited higher cyclin E levels than corresponding normal kidney cortex tissues. However, only a small fraction of the tumors (3 of 80) had excessive levels of cyclin E when... (More)
Aberrations in the GI-S transition have been observed in several malignancies, suggesting that cell cycle defects are linked to the activation of oncogenes and inactivation of suppressor genes involved in the transformation process. The frequency of GI/S aberrations in human renal cell carcinoma (RCC) has not been fully clarified. We have therefore analyzed the cyclin E content, using Western blotting, in 79 RCC and 12 corresponding kidney cortex tissues as well as the fraction of p27-positive cells in 73 RCCs, using immunohistochemistry. Most of the tumors (65%) exhibited higher cyclin E levels than corresponding normal kidney cortex tissues. However, only a small fraction of the tumors (3 of 80) had excessive levels of cyclin E when cyclin E levels were compared with proliferation. Cyclin E levels higher than the median value were associated with aneuploicly (p = 0.025), high stage (p = 0.027), high grade (p = 0.013) and high erythrocyte sedimentation rate (ESR; p = 0.005). Cyclin E was further inversely correlated with cyclin D1 (p = 0.023) and positively correlated with cyclin D3 (p = 0.003). Most tumors (76%) demonstrated a normal fraction of p27-positive cells. There was an inverse correlation between p27 positivity and tumor size (p = 0.007), despite a lack of correlation between p27 and proliferation. Patients with p27 low tumors had a poor survival (p = 0.002). There was no correlation between p27 and cyclin E levels. In summary, the results suggest that protein expression of cyclin E and/or p27 is linked to tumor behavior. (C) 2002 Wiley-Liss, Inc. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
p27, cyclin, cell cycle, renal cell carcinoma, Gl/S transition, protein
in
International Journal of Cancer
volume
102
issue
6
pages
601 - 607
publisher
John Wiley & Sons
external identifiers
  • wos:000179306200008
  • pmid:12448001
  • scopus:0037146777
ISSN
0020-7136
DOI
10.1002/ijc.10763
language
English
LU publication?
yes
id
cfa3c749-8a96-473f-a16c-c67f02283bbf (old id 323499)
date added to LUP
2007-10-15 09:08:01
date last changed
2017-10-22 03:51:51
@article{cfa3c749-8a96-473f-a16c-c67f02283bbf,
  abstract     = {Aberrations in the GI-S transition have been observed in several malignancies, suggesting that cell cycle defects are linked to the activation of oncogenes and inactivation of suppressor genes involved in the transformation process. The frequency of GI/S aberrations in human renal cell carcinoma (RCC) has not been fully clarified. We have therefore analyzed the cyclin E content, using Western blotting, in 79 RCC and 12 corresponding kidney cortex tissues as well as the fraction of p27-positive cells in 73 RCCs, using immunohistochemistry. Most of the tumors (65%) exhibited higher cyclin E levels than corresponding normal kidney cortex tissues. However, only a small fraction of the tumors (3 of 80) had excessive levels of cyclin E when cyclin E levels were compared with proliferation. Cyclin E levels higher than the median value were associated with aneuploicly (p = 0.025), high stage (p = 0.027), high grade (p = 0.013) and high erythrocyte sedimentation rate (ESR; p = 0.005). Cyclin E was further inversely correlated with cyclin D1 (p = 0.023) and positively correlated with cyclin D3 (p = 0.003). Most tumors (76%) demonstrated a normal fraction of p27-positive cells. There was an inverse correlation between p27 positivity and tumor size (p = 0.007), despite a lack of correlation between p27 and proliferation. Patients with p27 low tumors had a poor survival (p = 0.002). There was no correlation between p27 and cyclin E levels. In summary, the results suggest that protein expression of cyclin E and/or p27 is linked to tumor behavior. (C) 2002 Wiley-Liss, Inc.},
  author       = {Hedberg, Y and Davoodi, E and Ljungberg, B and Roos, G and Landberg, Göran},
  issn         = {0020-7136},
  keyword      = {p27,cyclin,cell cycle,renal cell carcinoma,Gl/S transition,protein},
  language     = {eng},
  number       = {6},
  pages        = {601--607},
  publisher    = {John Wiley & Sons},
  series       = {International Journal of Cancer},
  title        = {Cyclin E and P27 protein content in human renal cell carcinoma: Clinical outcome and associations with cyclin D},
  url          = {http://dx.doi.org/10.1002/ijc.10763},
  volume       = {102},
  year         = {2002},
}