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Graphite electrodes modified with Neurospora crassa cellobiose dehydrogenase: Comparative electrochemical characterization under direct and mediated electron transfer

Kovacs, Gabor; Ortiz, Roberto LU ; Coman, Vasile; Harreither, Wolfgang; Popescu, Ionel Catalin; Ludwig, Roland and Gorton, Lo LU (2012) In Bioelectrochemistry 88. p.84-91
Abstract
The electrochemical characterization of a class II cellobiose dehydrogenase (CDH), from the ascomycete fungus Neurospora crassa. adsorbed on graphite (G), was performed in regard to direct (DET) and mediated electron transfer (MET). The effects of the applied potential, mediator (1,4 benzoquinone) concentration and flow carrier pH on the amperometric response of the G/CDH modified electrodes were investigated under flow conditions. From the calibration curves, recorded at two pH values (5.2 and 7.0) for nine different sugars, the kinetic and the analytical parameters were evaluated under DET and MET operation modes. These results together with those obtained from long term operational stability measurements showed that: (i) for all nine... (More)
The electrochemical characterization of a class II cellobiose dehydrogenase (CDH), from the ascomycete fungus Neurospora crassa. adsorbed on graphite (G), was performed in regard to direct (DET) and mediated electron transfer (MET). The effects of the applied potential, mediator (1,4 benzoquinone) concentration and flow carrier pH on the amperometric response of the G/CDH modified electrodes were investigated under flow conditions. From the calibration curves, recorded at two pH values (5.2 and 7.0) for nine different sugars, the kinetic and the analytical parameters were evaluated under DET and MET operation modes. These results together with those obtained from long term operational stability measurements showed that: (i) for all nine investigated sugars the sensitivity was higher for MET than for DET and for pH 5.2 compared to pH 7.0; (ii) irrespective of DET or MET operation mode, the sensitivity of the new enzyme towards the investigated sugars decreased in the following sequence: cellobiose > lactose > (cellotriose approximate to cellopentaose) > > (maltotriose approximate to maltotetraose approximate to maltopentaose) > (maltose approximate to glucose); (iii) for all tested substrates, the apparent CDH affinity was roughly higher in DET than in MET operation mode. (C) 2012 Elsevier B.V. All rights reserved. (Less)
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author
organization
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Contribution to journal
publication status
published
subject
keywords
Cellobiose dehydrogenase, Amperometric biosensors, Direct electron, transfer, Mediated electron transfer, Flow injection analysis
in
Bioelectrochemistry
volume
88
pages
84 - 91
publisher
Elsevier
external identifiers
  • wos:000311065000013
  • scopus:84863856097
ISSN
1878-562X
DOI
10.1016/j.bioelechem.2012.06.006
language
English
LU publication?
yes
id
8d45984b-d785-4313-960e-28c6cfdbdc3c (old id 3243050)
date added to LUP
2012-12-28 10:34:59
date last changed
2017-04-30 03:17:27
@article{8d45984b-d785-4313-960e-28c6cfdbdc3c,
  abstract     = {The electrochemical characterization of a class II cellobiose dehydrogenase (CDH), from the ascomycete fungus Neurospora crassa. adsorbed on graphite (G), was performed in regard to direct (DET) and mediated electron transfer (MET). The effects of the applied potential, mediator (1,4 benzoquinone) concentration and flow carrier pH on the amperometric response of the G/CDH modified electrodes were investigated under flow conditions. From the calibration curves, recorded at two pH values (5.2 and 7.0) for nine different sugars, the kinetic and the analytical parameters were evaluated under DET and MET operation modes. These results together with those obtained from long term operational stability measurements showed that: (i) for all nine investigated sugars the sensitivity was higher for MET than for DET and for pH 5.2 compared to pH 7.0; (ii) irrespective of DET or MET operation mode, the sensitivity of the new enzyme towards the investigated sugars decreased in the following sequence: cellobiose > lactose > (cellotriose approximate to cellopentaose) > > (maltotriose approximate to maltotetraose approximate to maltopentaose) > (maltose approximate to glucose); (iii) for all tested substrates, the apparent CDH affinity was roughly higher in DET than in MET operation mode. (C) 2012 Elsevier B.V. All rights reserved.},
  author       = {Kovacs, Gabor and Ortiz, Roberto and Coman, Vasile and Harreither, Wolfgang and Popescu, Ionel Catalin and Ludwig, Roland and Gorton, Lo},
  issn         = {1878-562X},
  keyword      = {Cellobiose dehydrogenase,Amperometric biosensors,Direct electron,transfer,Mediated electron transfer,Flow injection analysis},
  language     = {eng},
  pages        = {84--91},
  publisher    = {Elsevier},
  series       = {Bioelectrochemistry},
  title        = {Graphite electrodes modified with Neurospora crassa cellobiose dehydrogenase: Comparative electrochemical characterization under direct and mediated electron transfer},
  url          = {http://dx.doi.org/10.1016/j.bioelechem.2012.06.006},
  volume       = {88},
  year         = {2012},
}