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Matrilin-2 interacts with itself and with other extracellular matrix proteins

Piecha, D; Wiberg, C; Mörgelin, Matthias LU ; Reinhardt, DP; Deak, F; Maurer, P and Paulsson, M (2002) In Biochemical Journal 367(3). p.715-721
Abstract
Matrilin-2 is a component of extracellular filamentous networks. To study the interactions by which it can be integrated into such assemblies, full-length and truncated forms of matrilin-2 were recombinantly expressed in HEK-293 cells and purified from conditioned medium. The recombinant proteins, when used in interaction assays, showed affinity to matrilin-2 itself, but also to other collagenous and non-collagenous extracellular matrix proteins. The interaction between matrilin-2 and collagen I was studied in greater detail and could be shown to occur at distinct sites on the collagen I molecule and to have a K-D of about 3 x 10(-8) M. Interactions with some non-collagenous protein ligands were even stronger, with matrilin-2 binding to... (More)
Matrilin-2 is a component of extracellular filamentous networks. To study the interactions by which it can be integrated into such assemblies, full-length and truncated forms of matrilin-2 were recombinantly expressed in HEK-293 cells and purified from conditioned medium. The recombinant proteins, when used in interaction assays, showed affinity to matrilin-2 itself, but also to other collagenous and non-collagenous extracellular matrix proteins. The interaction between matrilin-2 and collagen I was studied in greater detail and could be shown to occur at distinct sites on the collagen I molecule and to have a K-D of about 3 x 10(-8) M. Interactions with some non-collagenous protein ligands were even stronger, with matrilin-2 binding to fibrillin-2, fibronectin and laminin-1-nidogen-1 complexes, with K-D values in the range of 10(-8)-10(-11) M. Co-localization of matrilin-2 with these ligands in the dermal-epidermal basement membrane, in the microfibrils extending from the basement membrane into the dermis, and in the dermal extracellular matrix, indicates a physiological relevance of the interactions in the assembly of supramolecular extracellular matrix structures. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
fibronectin, collagen, fibrillin, laminin, skin
in
Biochemical Journal
volume
367
issue
3
pages
715 - 721
publisher
Portland Press Limited
external identifiers
  • wos:000179177900016
  • pmid:12180907
  • scopus:0036847005
ISSN
0264-6021
DOI
10.1042/BJ20021069
language
English
LU publication?
yes
id
10041d46-3d4c-49f4-9614-d1a970a78daf (old id 324313)
date added to LUP
2007-11-09 07:34:16
date last changed
2017-07-30 04:28:33
@article{10041d46-3d4c-49f4-9614-d1a970a78daf,
  abstract     = {Matrilin-2 is a component of extracellular filamentous networks. To study the interactions by which it can be integrated into such assemblies, full-length and truncated forms of matrilin-2 were recombinantly expressed in HEK-293 cells and purified from conditioned medium. The recombinant proteins, when used in interaction assays, showed affinity to matrilin-2 itself, but also to other collagenous and non-collagenous extracellular matrix proteins. The interaction between matrilin-2 and collagen I was studied in greater detail and could be shown to occur at distinct sites on the collagen I molecule and to have a K-D of about 3 x 10(-8) M. Interactions with some non-collagenous protein ligands were even stronger, with matrilin-2 binding to fibrillin-2, fibronectin and laminin-1-nidogen-1 complexes, with K-D values in the range of 10(-8)-10(-11) M. Co-localization of matrilin-2 with these ligands in the dermal-epidermal basement membrane, in the microfibrils extending from the basement membrane into the dermis, and in the dermal extracellular matrix, indicates a physiological relevance of the interactions in the assembly of supramolecular extracellular matrix structures.},
  author       = {Piecha, D and Wiberg, C and Mörgelin, Matthias and Reinhardt, DP and Deak, F and Maurer, P and Paulsson, M},
  issn         = {0264-6021},
  keyword      = {fibronectin,collagen,fibrillin,laminin,skin},
  language     = {eng},
  number       = {3},
  pages        = {715--721},
  publisher    = {Portland Press Limited},
  series       = {Biochemical Journal},
  title        = {Matrilin-2 interacts with itself and with other extracellular matrix proteins},
  url          = {http://dx.doi.org/10.1042/BJ20021069},
  volume       = {367},
  year         = {2002},
}