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Characterization of the protein-synthesis dependent adaptive acid tolerance response in Lactobacillus acidophilus

Lorca, G L; de Valdez, G F and Ljungh, Åsa LU (2002) In Journal of Molecular Microbiology and Biotechnology 4(6). p.525-532
Abstract
Exposure of L. acidophilus CRL 639 cells to sublethal adaptive acid conditions (pH 5.0 for 60 min) was found to confer protection against subsequent exposure to lethal pH (pH 3.0). Adaptation, which only occurred in complex media, was dependent on de novo protein synthesis and was inhibited by amino acid analogues. There was no modification in the protein synthesis rate during adaptation, but the protein degradation rate decreased. Synthesis of acid stress proteins may increase the stability of pre-existing proteins. By 2D-PAGE, induction of nine acid stress proteins and repression of several housekeeping proteins was observed. Putative heat shock proteins DnaK, DnaJ, GrpE, GroES and GroEL (70, 43, 24, 10 and 55 kDa, respectively) were... (More)
Exposure of L. acidophilus CRL 639 cells to sublethal adaptive acid conditions (pH 5.0 for 60 min) was found to confer protection against subsequent exposure to lethal pH (pH 3.0). Adaptation, which only occurred in complex media, was dependent on de novo protein synthesis and was inhibited by amino acid analogues. There was no modification in the protein synthesis rate during adaptation, but the protein degradation rate decreased. Synthesis of acid stress proteins may increase the stability of pre-existing proteins. By 2D-PAGE, induction of nine acid stress proteins and repression of several housekeeping proteins was observed. Putative heat shock proteins DnaK, DnaJ, GrpE, GroES and GroEL (70, 43, 24, 10 and 55 kDa, respectively) were among the proteins whose synthesis was induced in response to acid adaptation. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Molecular Microbiology and Biotechnology
volume
4
issue
6
pages
525 - 532
publisher
Karger
external identifiers
  • pmid:12432952
  • wos:000179012900003
  • scopus:0036844576
ISSN
1464-1801
language
English
LU publication?
yes
id
78498333-7b09-4cfa-a526-c1242a4f465c (old id 324448)
alternative location
http://www.horizonpress.com/jmmb/v/v4/60.pdf
date added to LUP
2007-11-02 09:48:47
date last changed
2017-05-28 04:16:47
@article{78498333-7b09-4cfa-a526-c1242a4f465c,
  abstract     = {Exposure of L. acidophilus CRL 639 cells to sublethal adaptive acid conditions (pH 5.0 for 60 min) was found to confer protection against subsequent exposure to lethal pH (pH 3.0). Adaptation, which only occurred in complex media, was dependent on de novo protein synthesis and was inhibited by amino acid analogues. There was no modification in the protein synthesis rate during adaptation, but the protein degradation rate decreased. Synthesis of acid stress proteins may increase the stability of pre-existing proteins. By 2D-PAGE, induction of nine acid stress proteins and repression of several housekeeping proteins was observed. Putative heat shock proteins DnaK, DnaJ, GrpE, GroES and GroEL (70, 43, 24, 10 and 55 kDa, respectively) were among the proteins whose synthesis was induced in response to acid adaptation.},
  author       = {Lorca, G L and de Valdez, G F and Ljungh, Åsa},
  issn         = {1464-1801},
  language     = {eng},
  number       = {6},
  pages        = {525--532},
  publisher    = {Karger},
  series       = {Journal of Molecular Microbiology and Biotechnology},
  title        = {Characterization of the protein-synthesis dependent adaptive acid tolerance response in Lactobacillus acidophilus},
  volume       = {4},
  year         = {2002},
}