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Recombinant pyranose dehydrogenase-A versatile enzyme possessing both mediated and direct electron transfer

Yakovleva, Maria LU ; Killyeni, Aniko; Ortiz, Roberto LU ; Schulz, Christopher LU ; MacAodha, Domhnall; Conghaile, Peter O.; Leech, Donal; Popescu, Ionel Catalin; Gonaus, Christoph and Peterbauer, Clemens K., et al. (2012) In Electrochemistry Communications 24. p.120-122
Abstract
The catalytical properties of glycosylated pyranose dehydrogenase (gPDH) and deglycosylated PDH (dgPDH) from Agaricus meleagris recombinantly expressed in Pichia pastoris were studied. Both gPDH and dgPDH were "wired" to an osmium redox polymer on graphite electrodes mounted in a flow-injection system. The current from oxidation of glucose by immobilised gPDH and dgPDH was compared using flow injection amperometry and cyclic voltammetry. An increase in the current density was observed for dgPDH (190 mu A cm(-2)) compared with that for gPDH (90 mu A cm(-2)) due to the improved electron transfer between the active site and the electrode. Additionally, the ability of dgPDH for direct electron transfer (DET) was discovered, which is rather... (More)
The catalytical properties of glycosylated pyranose dehydrogenase (gPDH) and deglycosylated PDH (dgPDH) from Agaricus meleagris recombinantly expressed in Pichia pastoris were studied. Both gPDH and dgPDH were "wired" to an osmium redox polymer on graphite electrodes mounted in a flow-injection system. The current from oxidation of glucose by immobilised gPDH and dgPDH was compared using flow injection amperometry and cyclic voltammetry. An increase in the current density was observed for dgPDH (190 mu A cm(-2)) compared with that for gPDH (90 mu A cm(-2)) due to the improved electron transfer between the active site and the electrode. Additionally, the ability of dgPDH for direct electron transfer (DET) was discovered, which is rather unique among FAD-containing enzymes. The ability to oxidise a variety of sugars at a rather low potential makes dgPDH attractive for construction of biofuel cells with high power output. (C) 2012 Elsevier B.V. All rights reserved. (Less)
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keywords
Pyranose dehydrogenase, Deglycosylation, Osmium polymer, Direct electron, transfer, Biosensor
in
Electrochemistry Communications
volume
24
pages
120 - 122
publisher
Elsevier
external identifiers
  • wos:000310943100032
  • scopus:84866695222
ISSN
1388-2481
DOI
10.1016/j.elecom.2012.08.029
language
English
LU publication?
yes
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3b00d7a5-688d-4520-8649-e444f892934a (old id 3244530)
date added to LUP
2012-12-27 12:36:08
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2017-04-30 04:40:25
@article{3b00d7a5-688d-4520-8649-e444f892934a,
  abstract     = {The catalytical properties of glycosylated pyranose dehydrogenase (gPDH) and deglycosylated PDH (dgPDH) from Agaricus meleagris recombinantly expressed in Pichia pastoris were studied. Both gPDH and dgPDH were "wired" to an osmium redox polymer on graphite electrodes mounted in a flow-injection system. The current from oxidation of glucose by immobilised gPDH and dgPDH was compared using flow injection amperometry and cyclic voltammetry. An increase in the current density was observed for dgPDH (190 mu A cm(-2)) compared with that for gPDH (90 mu A cm(-2)) due to the improved electron transfer between the active site and the electrode. Additionally, the ability of dgPDH for direct electron transfer (DET) was discovered, which is rather unique among FAD-containing enzymes. The ability to oxidise a variety of sugars at a rather low potential makes dgPDH attractive for construction of biofuel cells with high power output. (C) 2012 Elsevier B.V. All rights reserved.},
  author       = {Yakovleva, Maria and Killyeni, Aniko and Ortiz, Roberto and Schulz, Christopher and MacAodha, Domhnall and Conghaile, Peter O. and Leech, Donal and Popescu, Ionel Catalin and Gonaus, Christoph and Peterbauer, Clemens K. and Gorton, Lo},
  issn         = {1388-2481},
  keyword      = {Pyranose dehydrogenase,Deglycosylation,Osmium polymer,Direct electron,transfer,Biosensor},
  language     = {eng},
  pages        = {120--122},
  publisher    = {Elsevier},
  series       = {Electrochemistry Communications},
  title        = {Recombinant pyranose dehydrogenase-A versatile enzyme possessing both mediated and direct electron transfer},
  url          = {http://dx.doi.org/10.1016/j.elecom.2012.08.029},
  volume       = {24},
  year         = {2012},
}