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Identification of a novel calreticulin isoform (Crt2) in human and mouse

Persson, Staffan LU ; Rosenquist, M and Sommarin, Marianne LU (2002) In Gene 297(1-2). p.151-158
Abstract
Calreticulin is a Ca2+-binding chaperone localized mainly in the endoplasmic/sarcoplasmic reticulum in all higher organisms. To date, only one calreticulin isoform has been identified in human and mouse. Here we report a novel calreticulin isoform (Crt2) in human and mouse, with 53 (human) and 49% (mouse) identity to the previously identified calreticulin in respective species. The gene encoding the novel human calreticulin isoform spans 17 kb of genomic DNA and is expressed in testis, showing a similar expression as the chaperone calmegin. Phylogenetic analysis shows that two or more calreticulin (crt) genes are present both in plants and in mammals. The duplication of the crt gene in human and mouse suggests functional diversity, and... (More)
Calreticulin is a Ca2+-binding chaperone localized mainly in the endoplasmic/sarcoplasmic reticulum in all higher organisms. To date, only one calreticulin isoform has been identified in human and mouse. Here we report a novel calreticulin isoform (Crt2) in human and mouse, with 53 (human) and 49% (mouse) identity to the previously identified calreticulin in respective species. The gene encoding the novel human calreticulin isoform spans 17 kb of genomic DNA and is expressed in testis, showing a similar expression as the chaperone calmegin. Phylogenetic analysis shows that two or more calreticulin (crt) genes are present both in plants and in mammals. The duplication of the crt gene in human and mouse suggests functional diversity, and variations in expression patterns among calreticulins. Two novel calreticulin (Crt2) isoforms, with high homology to the human and mouse calreticulin isoform (Crt2), were also identified in pig and rat via expressed sequence tags. (C) 2002 Elsevier Science B.V. All rights reserved. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
endoplasmic reticulum, calmegin, calnexin, calcium, chaperone
in
Gene
volume
297
issue
1-2
pages
151 - 158
publisher
Elsevier
external identifiers
  • pmid:12384296
  • wos:000179075600017
  • scopus:0037019814
ISSN
1879-0038
DOI
10.1016/S0378-1119(02)00880-6
language
English
LU publication?
yes
id
eefaf119-4155-40f2-965d-9ba04fe819cc (old id 324525)
date added to LUP
2007-11-08 14:35:33
date last changed
2017-09-24 04:18:55
@article{eefaf119-4155-40f2-965d-9ba04fe819cc,
  abstract     = {Calreticulin is a Ca2+-binding chaperone localized mainly in the endoplasmic/sarcoplasmic reticulum in all higher organisms. To date, only one calreticulin isoform has been identified in human and mouse. Here we report a novel calreticulin isoform (Crt2) in human and mouse, with 53 (human) and 49% (mouse) identity to the previously identified calreticulin in respective species. The gene encoding the novel human calreticulin isoform spans 17 kb of genomic DNA and is expressed in testis, showing a similar expression as the chaperone calmegin. Phylogenetic analysis shows that two or more calreticulin (crt) genes are present both in plants and in mammals. The duplication of the crt gene in human and mouse suggests functional diversity, and variations in expression patterns among calreticulins. Two novel calreticulin (Crt2) isoforms, with high homology to the human and mouse calreticulin isoform (Crt2), were also identified in pig and rat via expressed sequence tags. (C) 2002 Elsevier Science B.V. All rights reserved.},
  author       = {Persson, Staffan and Rosenquist, M and Sommarin, Marianne},
  issn         = {1879-0038},
  keyword      = {endoplasmic reticulum,calmegin,calnexin,calcium,chaperone},
  language     = {eng},
  number       = {1-2},
  pages        = {151--158},
  publisher    = {Elsevier},
  series       = {Gene},
  title        = {Identification of a novel calreticulin isoform (Crt2) in human and mouse},
  url          = {http://dx.doi.org/10.1016/S0378-1119(02)00880-6},
  volume       = {297},
  year         = {2002},
}