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Activation of c-Src is inversely correlated with biological aggressiveness of breast carcinoma

Ito, Y; Kawakatsu, H; Takeda, T; Tani, N; Kawaguchi, N; Noguchi, S; Sakai, Takao LU and Matsuura, N (2002) In Breast Cancer Research and Treatment 76(3). p.261-267
Abstract
In order to investigate whether c-Src is involved in carcinogenesis and progression of breast carcinoma, we examined the expression of activated c-Src in tissue sections from surgically resected human breast specimens. First, we confirmed the specificity of the antibody against activated c-Src (Clone 28) using six cell lines established from human breast carcinomas by western blotting. As expected, activated c-Src was detected as a 60 kDa band in all cell lines tested. Immunofluorescence analysis demonstrated that the activated c-Src was mainly observed in cytoplasms of these cells. Then, we designed an immunohistochemical study with 73 human breast carcinoma tissues. Glandular epithelial and myoepithelial cells in normal mammary glands... (More)
In order to investigate whether c-Src is involved in carcinogenesis and progression of breast carcinoma, we examined the expression of activated c-Src in tissue sections from surgically resected human breast specimens. First, we confirmed the specificity of the antibody against activated c-Src (Clone 28) using six cell lines established from human breast carcinomas by western blotting. As expected, activated c-Src was detected as a 60 kDa band in all cell lines tested. Immunofluorescence analysis demonstrated that the activated c-Src was mainly observed in cytoplasms of these cells. Then, we designed an immunohistochemical study with 73 human breast carcinoma tissues. Glandular epithelial and myoepithelial cells in normal mammary glands adjacent to carcinoma nests and infiltrating stromal cells were negative for activated c-Src. In contrast, 37 of the 73 breast carcinoma tested (50.7%) were positive for activated c-Src, and this positive staining was inversely correlated with Ki-67 labeling index (p <0.0001), TNM stage (p <0.0001), tumor size (p < 0.0001), and histological grade (p = 0.0002). These results strongly suggest that the activation of c-Src would be related to the progression of breast carcinomas with low aggressiveness. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
src, breast carcinoma, immunohistochemistry
in
Breast Cancer Research and Treatment
volume
76
issue
3
pages
261 - 267
publisher
Springer
external identifiers
  • wos:000178929800009
  • pmid:12462387
  • scopus:0036890288
ISSN
1573-7217
DOI
10.1023/A:1020860221099
language
English
LU publication?
yes
id
2da3765c-fc5e-450d-94eb-f6f0f0307d45 (old id 324598)
date added to LUP
2007-10-23 11:11:29
date last changed
2017-01-01 06:37:48
@article{2da3765c-fc5e-450d-94eb-f6f0f0307d45,
  abstract     = {In order to investigate whether c-Src is involved in carcinogenesis and progression of breast carcinoma, we examined the expression of activated c-Src in tissue sections from surgically resected human breast specimens. First, we confirmed the specificity of the antibody against activated c-Src (Clone 28) using six cell lines established from human breast carcinomas by western blotting. As expected, activated c-Src was detected as a 60 kDa band in all cell lines tested. Immunofluorescence analysis demonstrated that the activated c-Src was mainly observed in cytoplasms of these cells. Then, we designed an immunohistochemical study with 73 human breast carcinoma tissues. Glandular epithelial and myoepithelial cells in normal mammary glands adjacent to carcinoma nests and infiltrating stromal cells were negative for activated c-Src. In contrast, 37 of the 73 breast carcinoma tested (50.7%) were positive for activated c-Src, and this positive staining was inversely correlated with Ki-67 labeling index (p &lt;0.0001), TNM stage (p &lt;0.0001), tumor size (p &lt; 0.0001), and histological grade (p = 0.0002). These results strongly suggest that the activation of c-Src would be related to the progression of breast carcinomas with low aggressiveness.},
  author       = {Ito, Y and Kawakatsu, H and Takeda, T and Tani, N and Kawaguchi, N and Noguchi, S and Sakai, Takao and Matsuura, N},
  issn         = {1573-7217},
  keyword      = {src,breast carcinoma,immunohistochemistry},
  language     = {eng},
  number       = {3},
  pages        = {261--267},
  publisher    = {Springer},
  series       = {Breast Cancer Research and Treatment},
  title        = {Activation of c-Src is inversely correlated with biological aggressiveness of breast carcinoma},
  url          = {http://dx.doi.org/10.1023/A:1020860221099},
  volume       = {76},
  year         = {2002},
}