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Enterococcus faecalis heme-dependent catalase

Frankenberg, Lena LU ; Brugna, Myriam LU and Hederstedt, Lars LU (2002) In Journal of Bacteriology 184(22). p.6351-6356
Abstract
Enterococcus faecalis cells cannot synthesize porphyrins and do not rely on heme for growth but can take up heme and use it to synthesize heme proteins. We recently described a cytochrome bd in E. faecalis strain V583 and here report the identification of a chromosomal gene, katA, encoding a heme-containing cytoplasmic catalase. The 54-kDa KatA polypeptide shows sequence similarity to members of the family of monofunctional catalases. A hexahistidyl-tagged version of the catalase was purified, and major characteristics of the enzyme were determined. It contains one protoheme IX group per KatA polypeptide. Catalase activity was detected only in E. faecalis cells grown in the presence of heme in the medium; about 2 and 10 muM hemin was... (More)
Enterococcus faecalis cells cannot synthesize porphyrins and do not rely on heme for growth but can take up heme and use it to synthesize heme proteins. We recently described a cytochrome bd in E. faecalis strain V583 and here report the identification of a chromosomal gene, katA, encoding a heme-containing cytoplasmic catalase. The 54-kDa KatA polypeptide shows sequence similarity to members of the family of monofunctional catalases. A hexahistidyl-tagged version of the catalase was purified, and major characteristics of the enzyme were determined. It contains one protoheme IX group per KatA polypeptide. Catalase activity was detected only in E. faecalis cells grown in the presence of heme in the medium; about 2 and 10 muM hemin was required for half-maximal and maximal production of catalase, respectively. Our finding of a catalase whose synthesis is dependent on the acquisition of heme in the opportunistic pathogen E. faecalis might be of clinical importance. Studies of cellular heme transport and heme protein assembly and in vivo synthesis of metalloprotein analogs for biotechnological applications are impeded by the lack of experimental systems. We conclude that the E. faecalis cell potentially provides such a desired system. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Bacteriology
volume
184
issue
22
pages
6351 - 6356
publisher
American Society for Microbiology
external identifiers
  • wos:000178916700029
  • pmid:12399505
  • scopus:0036842062
ISSN
0021-9193
DOI
10.1128/JB.184.22.6351-6356.2002
language
English
LU publication?
yes
id
113648e1-daa5-494c-aa73-afda5503223d (old id 324764)
date added to LUP
2007-08-20 11:45:50
date last changed
2017-12-10 03:45:41
@article{113648e1-daa5-494c-aa73-afda5503223d,
  abstract     = {Enterococcus faecalis cells cannot synthesize porphyrins and do not rely on heme for growth but can take up heme and use it to synthesize heme proteins. We recently described a cytochrome bd in E. faecalis strain V583 and here report the identification of a chromosomal gene, katA, encoding a heme-containing cytoplasmic catalase. The 54-kDa KatA polypeptide shows sequence similarity to members of the family of monofunctional catalases. A hexahistidyl-tagged version of the catalase was purified, and major characteristics of the enzyme were determined. It contains one protoheme IX group per KatA polypeptide. Catalase activity was detected only in E. faecalis cells grown in the presence of heme in the medium; about 2 and 10 muM hemin was required for half-maximal and maximal production of catalase, respectively. Our finding of a catalase whose synthesis is dependent on the acquisition of heme in the opportunistic pathogen E. faecalis might be of clinical importance. Studies of cellular heme transport and heme protein assembly and in vivo synthesis of metalloprotein analogs for biotechnological applications are impeded by the lack of experimental systems. We conclude that the E. faecalis cell potentially provides such a desired system.},
  author       = {Frankenberg, Lena and Brugna, Myriam and Hederstedt, Lars},
  issn         = {0021-9193},
  language     = {eng},
  number       = {22},
  pages        = {6351--6356},
  publisher    = {American Society for Microbiology},
  series       = {Journal of Bacteriology},
  title        = {Enterococcus faecalis heme-dependent catalase},
  url          = {http://dx.doi.org/10.1128/JB.184.22.6351-6356.2002},
  volume       = {184},
  year         = {2002},
}