Increased CpG methylation of the estrogen receptor gene in BRCA1-linked estrogen receptor-negative breast cancers
(2002) In Oncogene 21(46). p.7034-7041- Abstract
- A distinctive feature of BRCA1-linked breast cancers is that they typically do not express estrogen receptor-alpha (ERalpha). Previous investigation suggests that methylation of CpGs within the ERa promoter mediates repression of gene expression in some ERalpha-negative breast cancers. To determine if methylation of CpGs within the ERalpha promoter is associated with BRCA1-linked breast cancers, we evaluated methylation in exon 1 of the ERalpha gene in 40 ERalpha-negative breast cancers, 20 of which were non BRCA1-linked and 20 BRCA1-linked. CpG methylation was evaluated by either methylation-sensitive restriction digest (HpaII), methylation-sensitive PCR (MSP), or direct sequencing of bisulfite-treated genomic DNA. Results from HpaII... (More)
- A distinctive feature of BRCA1-linked breast cancers is that they typically do not express estrogen receptor-alpha (ERalpha). Previous investigation suggests that methylation of CpGs within the ERa promoter mediates repression of gene expression in some ERalpha-negative breast cancers. To determine if methylation of CpGs within the ERalpha promoter is associated with BRCA1-linked breast cancers, we evaluated methylation in exon 1 of the ERalpha gene in 40 ERalpha-negative breast cancers, 20 of which were non BRCA1-linked and 20 BRCA1-linked. CpG methylation was evaluated by either methylation-sensitive restriction digest (HpaII), methylation-sensitive PCR (MSP), or direct sequencing of bisulfite-treated genomic DNA. Results from HpaII digests and MSP documented a high degree of methylation, the MSP data showing slightly higher methylation in the BRCA1-linked group. CpGs analysed by direct sequencing showed an overall average methylation of 25% among non BRCA1-linked cancers and 40% among BRCA1-linked cancers (P=0.0031). The most notable difference was found at five particular CpGs, each of which exhibited a greater than twofold increase in methylation in the BRCA1-linked group compared to the non BRCA1-linked group (P < 0.03 for each CpG). Methylation of certain critical CpGs may represent an important factor in transcriptional repression of the ERa gene in BRCA1-linked breast cancers. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/325952
- author
- Archey, WB ; McEachern, KA ; Robson, M ; Offit, K ; Vaziri, SAJ ; Casey, G ; Borg, Åke LU and Arrick, BA
- organization
- publishing date
- 2002
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- methylation, BRCA1, breast cancer, estrogen receptor
- in
- Oncogene
- volume
- 21
- issue
- 46
- pages
- 7034 - 7041
- publisher
- Nature Publishing Group
- external identifiers
-
- wos:000178424900006
- pmid:12370825
- scopus:0037057324
- pmid:12370825
- ISSN
- 1476-5594
- DOI
- 10.1038/sj.onc.1205844
- language
- English
- LU publication?
- yes
- id
- 5b8cc249-4359-431e-8fe0-464f03ef09b1 (old id 325952)
- date added to LUP
- 2016-04-01 11:42:02
- date last changed
- 2022-01-26 08:56:04
@article{5b8cc249-4359-431e-8fe0-464f03ef09b1, abstract = {{A distinctive feature of BRCA1-linked breast cancers is that they typically do not express estrogen receptor-alpha (ERalpha). Previous investigation suggests that methylation of CpGs within the ERa promoter mediates repression of gene expression in some ERalpha-negative breast cancers. To determine if methylation of CpGs within the ERalpha promoter is associated with BRCA1-linked breast cancers, we evaluated methylation in exon 1 of the ERalpha gene in 40 ERalpha-negative breast cancers, 20 of which were non BRCA1-linked and 20 BRCA1-linked. CpG methylation was evaluated by either methylation-sensitive restriction digest (HpaII), methylation-sensitive PCR (MSP), or direct sequencing of bisulfite-treated genomic DNA. Results from HpaII digests and MSP documented a high degree of methylation, the MSP data showing slightly higher methylation in the BRCA1-linked group. CpGs analysed by direct sequencing showed an overall average methylation of 25% among non BRCA1-linked cancers and 40% among BRCA1-linked cancers (P=0.0031). The most notable difference was found at five particular CpGs, each of which exhibited a greater than twofold increase in methylation in the BRCA1-linked group compared to the non BRCA1-linked group (P < 0.03 for each CpG). Methylation of certain critical CpGs may represent an important factor in transcriptional repression of the ERa gene in BRCA1-linked breast cancers.}}, author = {{Archey, WB and McEachern, KA and Robson, M and Offit, K and Vaziri, SAJ and Casey, G and Borg, Åke and Arrick, BA}}, issn = {{1476-5594}}, keywords = {{methylation; BRCA1; breast cancer; estrogen receptor}}, language = {{eng}}, number = {{46}}, pages = {{7034--7041}}, publisher = {{Nature Publishing Group}}, series = {{Oncogene}}, title = {{Increased CpG methylation of the estrogen receptor gene in BRCA1-linked estrogen receptor-negative breast cancers}}, url = {{http://dx.doi.org/10.1038/sj.onc.1205844}}, doi = {{10.1038/sj.onc.1205844}}, volume = {{21}}, year = {{2002}}, }