Steroid transformation using magnetically immobilized Mycobacterium sp.
(1987) In Enzyme and Microbial Technology 9(8). p.494-499- Abstract
- Mycobacterium sp. (NRRL-B 3683) has been immobilized by adhesion of magnetic materials of submicron size to the bacterial surface. Preparations based on laboratory-prepared magnetic oxide that had been derivatized with hydrophobic octyltrichlorosilane showed the best properties. The magnetically immobilized bacteria were used for side-chain degradation of cholesterol into androsta-1,4-diene-3,17-dione. The magnetic bacteria behaved as free cells in the transformation media and no mass transfer limitations were observed. The magnetic bacteria could be used repeatedly without any cell loss, the cells being retrieved at the end of each transformation cycle by a magnet.
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https://lup.lub.lu.se/record/325a3479-3c7e-4c32-b684-7b46b602f40b
- author
- Flygare, Susanne and Larsson, Per-Olof LU
- organization
- publishing date
- 1987
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Steroid transformation, Magnetic bacteria, Magnetic immobilization, Side chain degradation, Mycobacterium sp.
- in
- Enzyme and Microbial Technology
- volume
- 9
- issue
- 8
- pages
- 6 pages
- publisher
- Elsevier
- external identifiers
-
- scopus:0023399982
- ISSN
- 0141-0229
- DOI
- 10.1016/0141-0229(87)90104-9
- language
- English
- LU publication?
- yes
- id
- 325a3479-3c7e-4c32-b684-7b46b602f40b
- date added to LUP
- 2024-06-18 14:03:11
- date last changed
- 2024-09-06 12:54:47
@article{325a3479-3c7e-4c32-b684-7b46b602f40b,
abstract = {{Mycobacterium sp. (NRRL-B 3683) has been immobilized by adhesion of magnetic materials of submicron size to the bacterial surface. Preparations based on laboratory-prepared magnetic oxide that had been derivatized with hydrophobic octyltrichlorosilane showed the best properties. The magnetically immobilized bacteria were used for side-chain degradation of cholesterol into androsta-1,4-diene-3,17-dione. The magnetic bacteria behaved as free cells in the transformation media and no mass transfer limitations were observed. The magnetic bacteria could be used repeatedly without any cell loss, the cells being retrieved at the end of each transformation cycle by a magnet.}},
author = {{Flygare, Susanne and Larsson, Per-Olof}},
issn = {{0141-0229}},
keywords = {{Steroid transformation; Magnetic bacteria; Magnetic immobilization; Side chain degradation; Mycobacterium sp.}},
language = {{eng}},
number = {{8}},
pages = {{494--499}},
publisher = {{Elsevier}},
series = {{Enzyme and Microbial Technology}},
title = {{Steroid transformation using magnetically immobilized <i>Mycobacterium sp</i>.}},
url = {{http://dx.doi.org/10.1016/0141-0229(87)90104-9}},
doi = {{10.1016/0141-0229(87)90104-9}},
volume = {{9}},
year = {{1987}},
}