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Binding of extracellular matrix molecules by staphylococci from wild herbivores

Styriak, I; Laukova, A and Ljungh, Åsa LU (2002) In Acta Veterinaria Brno 71(3). p.369-374
Abstract
Nine strains of staphylococci were examined for their antibiotic resistance and for binding of seven extracellular matrix (ECM) molecules (bovine mucin, porcine mucin, porcine fibronectin, bovine fibrinogen, fetuin, bovine lactoferrin and heparin) immobilized on latex beads in the particle agglutination assay. All nine strains were resistant to bacitracin, and most of them were multiresistant. Four strains displayed resistance to 5 of 13 antibiotics tested. Different binding capability between individual strains was observed. There were strains binding several ECM molecules (as S. warneri SW6 and S.. epidermidis SE14) as well as strains which bound only one (S. aureus SA 11) or no ECM molecule (S. saprophyticus SS16) of seven tested. While... (More)
Nine strains of staphylococci were examined for their antibiotic resistance and for binding of seven extracellular matrix (ECM) molecules (bovine mucin, porcine mucin, porcine fibronectin, bovine fibrinogen, fetuin, bovine lactoferrin and heparin) immobilized on latex beads in the particle agglutination assay. All nine strains were resistant to bacitracin, and most of them were multiresistant. Four strains displayed resistance to 5 of 13 antibiotics tested. Different binding capability between individual strains was observed. There were strains binding several ECM molecules (as S. warneri SW6 and S.. epidermidis SE14) as well as strains which bound only one (S. aureus SA 11) or no ECM molecule (S. saprophyticus SS16) of seven tested. While some ECM molecules, e.g. porcine fibronectin and heparin, were bound by most of strains, others (e.g. mucins) were bound only by I or 2 strains. Some strains bound these substrates weakly, however, other strains displayed strong binding especially of heparin and porcine fibronectin. The preincubation of bacteria for I hour at room temperature with a protein used subsequently in the PAA completely prevented the agglutination reaction, thus indicating the specificity of the assay. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
heparin, fetuin, fibrinogen, fibronectin, ECM binding, mucin, antibiotic resistance, lactoferrin
in
Acta Veterinaria Brno
volume
71
issue
3
pages
369 - 374
publisher
VYSOKA SKOLA VETERINARNI FARMACEUTICKA
external identifiers
  • wos:000178403400015
  • scopus:0344688371
ISSN
1801-7576
language
English
LU publication?
yes
id
eebedfb5-2d35-4f2d-b9e3-dc1d558e8ddf (old id 326249)
alternative location
http://vfu-www.vfu.cz/acta-vet/vol71/pdf/71_369.pdf
date added to LUP
2007-11-09 12:46:13
date last changed
2017-01-01 04:32:06
@article{eebedfb5-2d35-4f2d-b9e3-dc1d558e8ddf,
  abstract     = {Nine strains of staphylococci were examined for their antibiotic resistance and for binding of seven extracellular matrix (ECM) molecules (bovine mucin, porcine mucin, porcine fibronectin, bovine fibrinogen, fetuin, bovine lactoferrin and heparin) immobilized on latex beads in the particle agglutination assay. All nine strains were resistant to bacitracin, and most of them were multiresistant. Four strains displayed resistance to 5 of 13 antibiotics tested. Different binding capability between individual strains was observed. There were strains binding several ECM molecules (as S. warneri SW6 and S.. epidermidis SE14) as well as strains which bound only one (S. aureus SA 11) or no ECM molecule (S. saprophyticus SS16) of seven tested. While some ECM molecules, e.g. porcine fibronectin and heparin, were bound by most of strains, others (e.g. mucins) were bound only by I or 2 strains. Some strains bound these substrates weakly, however, other strains displayed strong binding especially of heparin and porcine fibronectin. The preincubation of bacteria for I hour at room temperature with a protein used subsequently in the PAA completely prevented the agglutination reaction, thus indicating the specificity of the assay.},
  author       = {Styriak, I and Laukova, A and Ljungh, Åsa},
  issn         = {1801-7576},
  keyword      = {heparin,fetuin,fibrinogen,fibronectin,ECM binding,mucin,antibiotic resistance,lactoferrin},
  language     = {eng},
  number       = {3},
  pages        = {369--374},
  publisher    = {VYSOKA SKOLA VETERINARNI FARMACEUTICKA},
  series       = {Acta Veterinaria Brno},
  title        = {Binding of extracellular matrix molecules by staphylococci from wild herbivores},
  volume       = {71},
  year         = {2002},
}