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Quantitative characterization of light-harvesting efficiency in single molecules and nanoparticles by 2D polarization microscopy: Experimental and theoretical challenges

Camacho Dejay, Rafael LU ; Thomsson, Daniel LU ; Yadav, Dheerendra LU and Scheblykin, Ivan LU (2012) In Chemical Physics 406. p.30-40
Abstract
General problem of extracting intramolecular energy transfer information from fluorescence and fluorescence excitation polarization experiments at single molecule level is presented. A single funnel approximation is shown to be a very powerful approach to model the polarization data obtained by recently emerged 2-dimensional polarization single molecule imaging technique [O. Mirzov et al., Small 5 (2009) 1877]. Using this approximation a parameter characterising quantitatively light-harvesting efficiency of an individual light-harvesting antenna can be readily obtained. Technical details of 2D polarization imaging and practical methods of avoiding polarization artefact in fluorescence microscopy are discussed. (C) 2012 Elsevier B. V. All... (More)
General problem of extracting intramolecular energy transfer information from fluorescence and fluorescence excitation polarization experiments at single molecule level is presented. A single funnel approximation is shown to be a very powerful approach to model the polarization data obtained by recently emerged 2-dimensional polarization single molecule imaging technique [O. Mirzov et al., Small 5 (2009) 1877]. Using this approximation a parameter characterising quantitatively light-harvesting efficiency of an individual light-harvesting antenna can be readily obtained. Technical details of 2D polarization imaging and practical methods of avoiding polarization artefact in fluorescence microscopy are discussed. (C) 2012 Elsevier B. V. All rights reserved. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Light-harvesting, Antenna, Single molecule spectroscopy, Anisotropy, Fluorescence, FRET, Energy funnelling
in
Chemical Physics
volume
406
pages
30 - 40
publisher
Elsevier
external identifiers
  • wos:000310569800006
  • scopus:84868207513
ISSN
0301-0104
DOI
10.1016/j.chemphys.2012.03.001
language
English
LU publication?
yes
id
cb37fa57-3389-4f11-8c52-0bb478f2061a (old id 3276237)
date added to LUP
2012-12-20 13:41:19
date last changed
2017-10-22 04:00:39
@article{cb37fa57-3389-4f11-8c52-0bb478f2061a,
  abstract     = {General problem of extracting intramolecular energy transfer information from fluorescence and fluorescence excitation polarization experiments at single molecule level is presented. A single funnel approximation is shown to be a very powerful approach to model the polarization data obtained by recently emerged 2-dimensional polarization single molecule imaging technique [O. Mirzov et al., Small 5 (2009) 1877]. Using this approximation a parameter characterising quantitatively light-harvesting efficiency of an individual light-harvesting antenna can be readily obtained. Technical details of 2D polarization imaging and practical methods of avoiding polarization artefact in fluorescence microscopy are discussed. (C) 2012 Elsevier B. V. All rights reserved.},
  author       = {Camacho Dejay, Rafael and Thomsson, Daniel and Yadav, Dheerendra and Scheblykin, Ivan},
  issn         = {0301-0104},
  keyword      = {Light-harvesting,Antenna,Single molecule spectroscopy,Anisotropy,Fluorescence,FRET,Energy funnelling},
  language     = {eng},
  pages        = {30--40},
  publisher    = {Elsevier},
  series       = {Chemical Physics},
  title        = {Quantitative characterization of light-harvesting efficiency in single molecules and nanoparticles by 2D polarization microscopy: Experimental and theoretical challenges},
  url          = {http://dx.doi.org/10.1016/j.chemphys.2012.03.001},
  volume       = {406},
  year         = {2012},
}