Preparation of a crystallizable mRNA-binding fragment of Moorella thermoacetica elongation factor SelB
(2002) In Acta Crystallographica. Section D: Biological Crystallography 58. p.1871-1873- Abstract
- SelB is a bacterial elongation factor required for the decoding of a UGA stop codon together with a specific mRNA hairpin to selenocysteine. In attempts to crystallize Moorella thermoacetica SelB, a proteolysis process occurred and crystals of a proteolytic fragment were observed. The crystals, which appeared after a year, contained a C-terminal 30 kDa fragment containing the mRNA-binding domain. This fragment was reproduced through recloning. Crystals diffracting to 2.7 Angstrom were obtained.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/327765
- author
- Selmer, Maria LU ; Wilting, R ; Holmlund, D and Su, Xiao-Dong LU
- organization
- publishing date
- 2002
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Acta Crystallographica. Section D: Biological Crystallography
- volume
- 58
- pages
- 1871 - 1873
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- wos:000178248100024
- pmid:12351842
- scopus:0036795489
- ISSN
- 1399-0047
- DOI
- 10.1107/S090744490201380X
- language
- English
- LU publication?
- yes
- id
- 4776c737-bcf5-4102-a095-a8fc6b862b3c (old id 327765)
- date added to LUP
- 2016-04-01 17:09:38
- date last changed
- 2022-01-29 00:44:42
@article{4776c737-bcf5-4102-a095-a8fc6b862b3c, abstract = {{SelB is a bacterial elongation factor required for the decoding of a UGA stop codon together with a specific mRNA hairpin to selenocysteine. In attempts to crystallize Moorella thermoacetica SelB, a proteolysis process occurred and crystals of a proteolytic fragment were observed. The crystals, which appeared after a year, contained a C-terminal 30 kDa fragment containing the mRNA-binding domain. This fragment was reproduced through recloning. Crystals diffracting to 2.7 Angstrom were obtained.}}, author = {{Selmer, Maria and Wilting, R and Holmlund, D and Su, Xiao-Dong}}, issn = {{1399-0047}}, language = {{eng}}, pages = {{1871--1873}}, publisher = {{John Wiley & Sons Inc.}}, series = {{Acta Crystallographica. Section D: Biological Crystallography}}, title = {{Preparation of a crystallizable mRNA-binding fragment of Moorella thermoacetica elongation factor SelB}}, url = {{http://dx.doi.org/10.1107/S090744490201380X}}, doi = {{10.1107/S090744490201380X}}, volume = {{58}}, year = {{2002}}, }