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Preparation of a crystallizable mRNA-binding fragment of Moorella thermoacetica elongation factor SelB

Selmer, Maria LU ; Wilting, R; Holmlund, D and Su, Xiao-Dong LU (2002) In Acta Crystallographica. Section D: Biological Crystallography 58. p.1871-1873
Abstract
SelB is a bacterial elongation factor required for the decoding of a UGA stop codon together with a specific mRNA hairpin to selenocysteine. In attempts to crystallize Moorella thermoacetica SelB, a proteolysis process occurred and crystals of a proteolytic fragment were observed. The crystals, which appeared after a year, contained a C-terminal 30 kDa fragment containing the mRNA-binding domain. This fragment was reproduced through recloning. Crystals diffracting to 2.7 Angstrom were obtained.
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Acta Crystallographica. Section D: Biological Crystallography
volume
58
pages
1871 - 1873
publisher
International Union of Crystallography
external identifiers
  • wos:000178248100024
  • pmid:12351842
  • scopus:0036795489
ISSN
1399-0047
DOI
10.1107/S090744490201380X
language
English
LU publication?
yes
id
4776c737-bcf5-4102-a095-a8fc6b862b3c (old id 327765)
date added to LUP
2007-11-13 14:39:24
date last changed
2017-01-01 07:25:56
@article{4776c737-bcf5-4102-a095-a8fc6b862b3c,
  abstract     = {SelB is a bacterial elongation factor required for the decoding of a UGA stop codon together with a specific mRNA hairpin to selenocysteine. In attempts to crystallize Moorella thermoacetica SelB, a proteolysis process occurred and crystals of a proteolytic fragment were observed. The crystals, which appeared after a year, contained a C-terminal 30 kDa fragment containing the mRNA-binding domain. This fragment was reproduced through recloning. Crystals diffracting to 2.7 Angstrom were obtained.},
  author       = {Selmer, Maria and Wilting, R and Holmlund, D and Su, Xiao-Dong},
  issn         = {1399-0047},
  language     = {eng},
  pages        = {1871--1873},
  publisher    = {International Union of Crystallography},
  series       = {Acta Crystallographica. Section D: Biological Crystallography},
  title        = {Preparation of a crystallizable mRNA-binding fragment of Moorella thermoacetica elongation factor SelB},
  url          = {http://dx.doi.org/10.1107/S090744490201380X},
  volume       = {58},
  year         = {2002},
}