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Population dynamics in wastewater treatment plants with enhanced biological phosphorus removal operated with and without nitrogen removal

Lee, N; la Cour Jansen, Jes LU ; Aspegren, H; Henze, M; Nielsen, PH and Wagner, M (2002) In Water Science and Technology 46(1-2). p.163-170
Abstract
The population dynamics of activated sludge in a pilot plant with two activated sludge systems, both designed for enhanced biological phosphorus removal (EBPR), but one of them with (BNP) and the other without (BP) nitrogen removal, was monitored during a period of 2.5 years. The influent water to the pilot plant was periodically manipulated by external addition of phosphorus (P), acetate and glucose, respectively. The population dynamics and the in situ physiology were monitored by quantitative fluorescence in situ hybridization (FISH) and microautoradiography. Significant P removal was observed in both systems throughout the whole period, with significant increases of the P removal when substrates were dosed. The activated sludge in both... (More)
The population dynamics of activated sludge in a pilot plant with two activated sludge systems, both designed for enhanced biological phosphorus removal (EBPR), but one of them with (BNP) and the other without (BP) nitrogen removal, was monitored during a period of 2.5 years. The influent water to the pilot plant was periodically manipulated by external addition of phosphorus (P), acetate and glucose, respectively. The population dynamics and the in situ physiology were monitored by quantitative fluorescence in situ hybridization (FISH) and microautoradiography. Significant P removal was observed in both systems throughout the whole period, with significant increases of the P removal when substrates were dosed. The activated sludge in both systems contained large amounts of dense clusters of gram-negative, methylene-blue staining coccoid rods during the whole period. A large part of the clusters belonged to the beta Proteobacteria, whereas the rest of the clusters belonged either to the Actinobacteria or to the a Proteobacteria. The relative abundance of Rhodocyclus-related bacteria in the activated sludge varied significantly in both systems during the whole period (from 6 to 18% in BNP, and from 4 to 28% in BP). However, no statistically significant correlation of the Rhodocyclus-related nor any of the other investigated bacterial groups to the P content of the activated sludge (correlation for all groups investigated was always <0.5) was observed. A significant P-33(i) uptake was observed by the β Proteobacteria (part of them Rhodocyclus-related, the identity of the rest unknown) and the Actinobacteria. However, not all of the Rhodocyclus-related bacteria showed P-33(i) uptake. The P removal in the investigated plants is thus believed to be mediated by a mixed population consisting of a part of the Rhodocyclus-related bacteria, the Actinobacteria and other, yet unidentified bacteria. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
polyphosphate-accumulating, activated sludge, nutrient removal, organisms (PAOs), Rhodocyclus
in
Water Science and Technology
volume
46
issue
1-2
pages
163 - 170
publisher
IWA Publishing
external identifiers
  • wos:000177989100026
  • pmid:12216618
  • scopus:0036353931
ISSN
0273-1223
language
English
LU publication?
yes
id
5c1d2d50-93df-4681-a671-225c3bc98069 (old id 327791)
date added to LUP
2007-11-14 12:58:15
date last changed
2017-11-05 04:25:20
@article{5c1d2d50-93df-4681-a671-225c3bc98069,
  abstract     = {The population dynamics of activated sludge in a pilot plant with two activated sludge systems, both designed for enhanced biological phosphorus removal (EBPR), but one of them with (BNP) and the other without (BP) nitrogen removal, was monitored during a period of 2.5 years. The influent water to the pilot plant was periodically manipulated by external addition of phosphorus (P), acetate and glucose, respectively. The population dynamics and the in situ physiology were monitored by quantitative fluorescence in situ hybridization (FISH) and microautoradiography. Significant P removal was observed in both systems throughout the whole period, with significant increases of the P removal when substrates were dosed. The activated sludge in both systems contained large amounts of dense clusters of gram-negative, methylene-blue staining coccoid rods during the whole period. A large part of the clusters belonged to the beta Proteobacteria, whereas the rest of the clusters belonged either to the Actinobacteria or to the a Proteobacteria. The relative abundance of Rhodocyclus-related bacteria in the activated sludge varied significantly in both systems during the whole period (from 6 to 18% in BNP, and from 4 to 28% in BP). However, no statistically significant correlation of the Rhodocyclus-related nor any of the other investigated bacterial groups to the P content of the activated sludge (correlation for all groups investigated was always &lt;0.5) was observed. A significant P-33(i) uptake was observed by the β Proteobacteria (part of them Rhodocyclus-related, the identity of the rest unknown) and the Actinobacteria. However, not all of the Rhodocyclus-related bacteria showed P-33(i) uptake. The P removal in the investigated plants is thus believed to be mediated by a mixed population consisting of a part of the Rhodocyclus-related bacteria, the Actinobacteria and other, yet unidentified bacteria.},
  author       = {Lee, N and la Cour Jansen, Jes and Aspegren, H and Henze, M and Nielsen, PH and Wagner, M},
  issn         = {0273-1223},
  keyword      = {polyphosphate-accumulating,activated sludge,nutrient removal,organisms (PAOs),Rhodocyclus},
  language     = {eng},
  number       = {1-2},
  pages        = {163--170},
  publisher    = {IWA Publishing},
  series       = {Water Science and Technology},
  title        = {Population dynamics in wastewater treatment plants with enhanced biological phosphorus removal operated with and without nitrogen removal},
  volume       = {46},
  year         = {2002},
}