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Acoustophoretic microfluidic chip for sequential elution of surface bound molecules from beads or cells

Augustsson, Per LU ; Malm, Johan LU and Ekström, Simon LU (2012) In Biomicrofluidics 6(3).
Abstract
An acoustophoresis-based microfluidic flow-chip is presented as a novel platform to facilitate analysis of proteins and peptides loosely bound to the surface of beads or cells. The chip allows for direct removal of the background surrounding the beads or cells, followed by sequential treatment and collection of a sequence of up to five different buffer conditions. During this treatment, the beads/cells are retained in a single flow by acoustic radiation force. Eluted peptides are collected from the outlets and subsequently purified by miniaturized solid-phase extraction and analyzed with matrix assisted laser desorption mass spectrometry. Fundamental parameters such as the system fluidics and dispersion are presented. The device was... (More)
An acoustophoresis-based microfluidic flow-chip is presented as a novel platform to facilitate analysis of proteins and peptides loosely bound to the surface of beads or cells. The chip allows for direct removal of the background surrounding the beads or cells, followed by sequential treatment and collection of a sequence of up to five different buffer conditions. During this treatment, the beads/cells are retained in a single flow by acoustic radiation force. Eluted peptides are collected from the outlets and subsequently purified by miniaturized solid-phase extraction and analyzed with matrix assisted laser desorption mass spectrometry. Fundamental parameters such as the system fluidics and dispersion are presented. The device was successfully applied for wash and sequential elution of peptides bound to the surface of microbeads and human spermatozoa, respectively. (C) 2012 American Institute of Physics. [http://dx.doi.org/10.1063/1.4749289] (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Biomicrofluidics
volume
6
issue
3
publisher
American Institute of Physics
external identifiers
  • wos:000309386900016
  • scopus:84867121906
ISSN
1932-1058
DOI
10.1063/1.4749289
language
English
LU publication?
yes
id
45a98ef4-19d8-4982-9fde-bcb7e440a343 (old id 3283970)
date added to LUP
2012-12-19 09:29:28
date last changed
2017-01-01 06:26:48
@article{45a98ef4-19d8-4982-9fde-bcb7e440a343,
  abstract     = {An acoustophoresis-based microfluidic flow-chip is presented as a novel platform to facilitate analysis of proteins and peptides loosely bound to the surface of beads or cells. The chip allows for direct removal of the background surrounding the beads or cells, followed by sequential treatment and collection of a sequence of up to five different buffer conditions. During this treatment, the beads/cells are retained in a single flow by acoustic radiation force. Eluted peptides are collected from the outlets and subsequently purified by miniaturized solid-phase extraction and analyzed with matrix assisted laser desorption mass spectrometry. Fundamental parameters such as the system fluidics and dispersion are presented. The device was successfully applied for wash and sequential elution of peptides bound to the surface of microbeads and human spermatozoa, respectively. (C) 2012 American Institute of Physics. [http://dx.doi.org/10.1063/1.4749289]},
  articleno    = {034115},
  author       = {Augustsson, Per and Malm, Johan and Ekström, Simon},
  issn         = {1932-1058},
  language     = {eng},
  number       = {3},
  publisher    = {American Institute of Physics},
  series       = {Biomicrofluidics},
  title        = {Acoustophoretic microfluidic chip for sequential elution of surface bound molecules from beads or cells},
  url          = {http://dx.doi.org/10.1063/1.4749289},
  volume       = {6},
  year         = {2012},
}