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Microwave-assisted extraction of zearalenone from wheat and corn

Pallaroni, L; von Holst, C; Eskilsson, C and Björklund, Erland LU (2002) In Analytical and Bioanalytical Chemistry 374(1). p.161-166
Abstract
A microwave-assisted extraction (MAE) method has been developed for determination of zearalenone in wheat and corn by LC-MS with an atmospheric pressure chemical ionization interface (APCI). Matrix effects were minimized by use of matrix-matched standard curves for quantification of the analyte. The limit of quantification (LOQ) of the method was 30 ng g(-1) in wheat and 20 ng g(-1) in corn. The rapid LC-MS method enabled analysis of the extracts without clean-up, thereby reducing analyte losses, the time required for the analytical procedure, and costs. A factorial design approach was used to examine the effect on extraction efficiency of the main extraction conditions - time, temperature, and solvent. On the basis of results from... (More)
A microwave-assisted extraction (MAE) method has been developed for determination of zearalenone in wheat and corn by LC-MS with an atmospheric pressure chemical ionization interface (APCI). Matrix effects were minimized by use of matrix-matched standard curves for quantification of the analyte. The limit of quantification (LOQ) of the method was 30 ng g(-1) in wheat and 20 ng g(-1) in corn. The rapid LC-MS method enabled analysis of the extracts without clean-up, thereby reducing analyte losses, the time required for the analytical procedure, and costs. A factorial design approach was used to examine the effect on extraction efficiency of the main extraction conditions - time, temperature, and solvent. On the basis of results from statistical assessment extraction was performed with 1:1 (v/v) methanol-acetonitrile at 80degreesC for 5 min. When these extraction conditions were applied to a wheat sample from a recently conducted international proficiency test, 92% (103 ng g(-1)) of the assigned zearalenone concentration (112 ng g(-1)) in the test material was obtained. This result was within the uncertainty (u) range of the assigned value of the test material (u = +/-15.8 ng g(-1), alpha = 0.05) thereby demonstrating the accuracy of the method was sufficient. The precision of the whole method was also confirmed to be adequate, because the observed relative standard deviation (RSD) of 12% (n = 10) also fulfils the quality criteria recommended by European guidelines for in-house method validation. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
food analysis, liquid, mycotoxin, zearalenone, microwave-assisted extraction, chromatography-mass spectrometry
in
Analytical and Bioanalytical Chemistry
volume
374
issue
1
pages
161 - 166
publisher
Springer
external identifiers
  • pmid:12207259
  • wos:000178112800028
  • scopus:0036042542
ISSN
1618-2642
DOI
10.1007/s00216-002-1426-4
language
English
LU publication?
yes
id
a41de864-7f30-4e52-a264-d5fdb0c81c35 (old id 328454)
date added to LUP
2007-11-08 09:23:11
date last changed
2017-08-13 03:35:21
@article{a41de864-7f30-4e52-a264-d5fdb0c81c35,
  abstract     = {A microwave-assisted extraction (MAE) method has been developed for determination of zearalenone in wheat and corn by LC-MS with an atmospheric pressure chemical ionization interface (APCI). Matrix effects were minimized by use of matrix-matched standard curves for quantification of the analyte. The limit of quantification (LOQ) of the method was 30 ng g(-1) in wheat and 20 ng g(-1) in corn. The rapid LC-MS method enabled analysis of the extracts without clean-up, thereby reducing analyte losses, the time required for the analytical procedure, and costs. A factorial design approach was used to examine the effect on extraction efficiency of the main extraction conditions - time, temperature, and solvent. On the basis of results from statistical assessment extraction was performed with 1:1 (v/v) methanol-acetonitrile at 80degreesC for 5 min. When these extraction conditions were applied to a wheat sample from a recently conducted international proficiency test, 92% (103 ng g(-1)) of the assigned zearalenone concentration (112 ng g(-1)) in the test material was obtained. This result was within the uncertainty (u) range of the assigned value of the test material (u = +/-15.8 ng g(-1), alpha = 0.05) thereby demonstrating the accuracy of the method was sufficient. The precision of the whole method was also confirmed to be adequate, because the observed relative standard deviation (RSD) of 12% (n = 10) also fulfils the quality criteria recommended by European guidelines for in-house method validation.},
  author       = {Pallaroni, L and von Holst, C and Eskilsson, C and Björklund, Erland},
  issn         = {1618-2642},
  keyword      = {food analysis,liquid,mycotoxin,zearalenone,microwave-assisted extraction,chromatography-mass spectrometry},
  language     = {eng},
  number       = {1},
  pages        = {161--166},
  publisher    = {Springer},
  series       = {Analytical and Bioanalytical Chemistry},
  title        = {Microwave-assisted extraction of zearalenone from wheat and corn},
  url          = {http://dx.doi.org/10.1007/s00216-002-1426-4},
  volume       = {374},
  year         = {2002},
}