Microwave-assisted extraction of zearalenone from wheat and corn
(2002) In Analytical and Bioanalytical Chemistry 374(1). p.161-166- Abstract
- A microwave-assisted extraction (MAE) method has been developed for determination of zearalenone in wheat and corn by LC-MS with an atmospheric pressure chemical ionization interface (APCI). Matrix effects were minimized by use of matrix-matched standard curves for quantification of the analyte. The limit of quantification (LOQ) of the method was 30 ng g(-1) in wheat and 20 ng g(-1) in corn. The rapid LC-MS method enabled analysis of the extracts without clean-up, thereby reducing analyte losses, the time required for the analytical procedure, and costs. A factorial design approach was used to examine the effect on extraction efficiency of the main extraction conditions - time, temperature, and solvent. On the basis of results from... (More)
- A microwave-assisted extraction (MAE) method has been developed for determination of zearalenone in wheat and corn by LC-MS with an atmospheric pressure chemical ionization interface (APCI). Matrix effects were minimized by use of matrix-matched standard curves for quantification of the analyte. The limit of quantification (LOQ) of the method was 30 ng g(-1) in wheat and 20 ng g(-1) in corn. The rapid LC-MS method enabled analysis of the extracts without clean-up, thereby reducing analyte losses, the time required for the analytical procedure, and costs. A factorial design approach was used to examine the effect on extraction efficiency of the main extraction conditions - time, temperature, and solvent. On the basis of results from statistical assessment extraction was performed with 1:1 (v/v) methanol-acetonitrile at 80degreesC for 5 min. When these extraction conditions were applied to a wheat sample from a recently conducted international proficiency test, 92% (103 ng g(-1)) of the assigned zearalenone concentration (112 ng g(-1)) in the test material was obtained. This result was within the uncertainty (u) range of the assigned value of the test material (u = +/-15.8 ng g(-1), alpha = 0.05) thereby demonstrating the accuracy of the method was sufficient. The precision of the whole method was also confirmed to be adequate, because the observed relative standard deviation (RSD) of 12% (n = 10) also fulfils the quality criteria recommended by European guidelines for in-house method validation. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/328454
- author
- Pallaroni, L ; von Holst, C ; Eskilsson, C and Björklund, Erland LU
- organization
- publishing date
- 2002
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- food analysis, liquid, mycotoxin, zearalenone, microwave-assisted extraction, chromatography-mass spectrometry
- in
- Analytical and Bioanalytical Chemistry
- volume
- 374
- issue
- 1
- pages
- 161 - 166
- publisher
- Springer
- external identifiers
-
- pmid:12207259
- wos:000178112800028
- scopus:0036042542
- ISSN
- 1618-2642
- DOI
- 10.1007/s00216-002-1426-4
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Analytical Chemistry (S/LTH) (011001004)
- id
- a41de864-7f30-4e52-a264-d5fdb0c81c35 (old id 328454)
- date added to LUP
- 2016-04-01 11:57:47
- date last changed
- 2022-04-28 22:34:13
@article{a41de864-7f30-4e52-a264-d5fdb0c81c35,
abstract = {{A microwave-assisted extraction (MAE) method has been developed for determination of zearalenone in wheat and corn by LC-MS with an atmospheric pressure chemical ionization interface (APCI). Matrix effects were minimized by use of matrix-matched standard curves for quantification of the analyte. The limit of quantification (LOQ) of the method was 30 ng g(-1) in wheat and 20 ng g(-1) in corn. The rapid LC-MS method enabled analysis of the extracts without clean-up, thereby reducing analyte losses, the time required for the analytical procedure, and costs. A factorial design approach was used to examine the effect on extraction efficiency of the main extraction conditions - time, temperature, and solvent. On the basis of results from statistical assessment extraction was performed with 1:1 (v/v) methanol-acetonitrile at 80degreesC for 5 min. When these extraction conditions were applied to a wheat sample from a recently conducted international proficiency test, 92% (103 ng g(-1)) of the assigned zearalenone concentration (112 ng g(-1)) in the test material was obtained. This result was within the uncertainty (u) range of the assigned value of the test material (u = +/-15.8 ng g(-1), alpha = 0.05) thereby demonstrating the accuracy of the method was sufficient. The precision of the whole method was also confirmed to be adequate, because the observed relative standard deviation (RSD) of 12% (n = 10) also fulfils the quality criteria recommended by European guidelines for in-house method validation.}},
author = {{Pallaroni, L and von Holst, C and Eskilsson, C and Björklund, Erland}},
issn = {{1618-2642}},
keywords = {{food analysis; liquid; mycotoxin; zearalenone; microwave-assisted extraction; chromatography-mass spectrometry}},
language = {{eng}},
number = {{1}},
pages = {{161--166}},
publisher = {{Springer}},
series = {{Analytical and Bioanalytical Chemistry}},
title = {{Microwave-assisted extraction of zearalenone from wheat and corn}},
url = {{http://dx.doi.org/10.1007/s00216-002-1426-4}},
doi = {{10.1007/s00216-002-1426-4}},
volume = {{374}},
year = {{2002}},
}