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Cytogenetic features of multiple myeloma: impact of gender, age, disease phase, culture time, and cytokine stimulation

Nilsson, Therese LU ; Lenhoff, S; Turesson, Ingemar LU ; Rylander, Lars LU ; Mitelman, Felix LU ; Westin, J; Höglund, Mattias LU and Johansson, Bertil LU (2002) In European Journal of Haematology1987-01-01+01:00 68(6). p.345-353
Abstract
Relatively little is known about the cytogenetic features of multiple myeloma (MM) when compared to other hematologic malignancies. The reasons for this are most likely manifold, and include a low mitotic index of the malignant cells and the presence of cytogenetically cryptic abnormalities as well as of complex karyotypes with poor chromosome morphology. In the present study, we have investigated whether various culture conditions may influence the yield of abnormal metaphases in MM and, in the related plasma cell dyscrasias, monoclonal gammopathy of undetermined significance (MGUS) and plasmacytomas (PC). In addition, the possible impact of age, gender, and disease phase on the cytogenetic features has been analyzed. A total of 95... (More)
Relatively little is known about the cytogenetic features of multiple myeloma (MM) when compared to other hematologic malignancies. The reasons for this are most likely manifold, and include a low mitotic index of the malignant cells and the presence of cytogenetically cryptic abnormalities as well as of complex karyotypes with poor chromosome morphology. In the present study, we have investigated whether various culture conditions may influence the yield of abnormal metaphases in MM and, in the related plasma cell dyscrasias, monoclonal gammopathy of undetermined significance (MGUS) and plasmacytomas (PC). In addition, the possible impact of age, gender, and disease phase on the cytogenetic features has been analyzed. A total of 95 samples from 74 cases (68 MM, three PC, and three MGUS patients) were obtained for cytogenetic analysis. The samples were cultured either in conventional medium or in medium containing IL-6 and GM-CSF, and the culture times varied from 24 to 120 h. In total, 186 cultures were analyzed. Metaphase fluorescence in situ hybridization analysis using probes specific for 14q32, i.e. IGH rearrangements, could be performed in 57 of the 74 cases, and revealed 14q32 aberrations in 10 cases not seen by conventional G-banding. Abnormal karyotypes were detected in 77 (41%) of the 186 cultures, 46 (48%) of the 95 samples, and in 41 (55%) of the 74 patients, revealing a total of 20 chromosomal aberrations previously not reported in plasma cell dyscrasias. We found no evidence that gender, age, disease phase, culture time, or cytokine stimulation significantly influences the karyotypic features of MM. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
disease phase, gender, age, multiple myeloma, cytogenetics, culture, conditions
in
European Journal of Haematology1987-01-01+01:00
volume
68
issue
6
pages
345 - 353
publisher
Wiley-Blackwell
external identifiers
  • pmid:12225392
  • wos:000177965100004
  • scopus:0036620705
ISSN
1600-0609
DOI
10.1034/j.1600-0609.2002.00724.x
language
English
LU publication?
yes
id
5faf56f5-c3ac-429a-ae81-da0a7f3f7749 (old id 329235)
date added to LUP
2007-11-16 10:44:56
date last changed
2017-01-01 04:33:07
@article{5faf56f5-c3ac-429a-ae81-da0a7f3f7749,
  abstract     = {Relatively little is known about the cytogenetic features of multiple myeloma (MM) when compared to other hematologic malignancies. The reasons for this are most likely manifold, and include a low mitotic index of the malignant cells and the presence of cytogenetically cryptic abnormalities as well as of complex karyotypes with poor chromosome morphology. In the present study, we have investigated whether various culture conditions may influence the yield of abnormal metaphases in MM and, in the related plasma cell dyscrasias, monoclonal gammopathy of undetermined significance (MGUS) and plasmacytomas (PC). In addition, the possible impact of age, gender, and disease phase on the cytogenetic features has been analyzed. A total of 95 samples from 74 cases (68 MM, three PC, and three MGUS patients) were obtained for cytogenetic analysis. The samples were cultured either in conventional medium or in medium containing IL-6 and GM-CSF, and the culture times varied from 24 to 120 h. In total, 186 cultures were analyzed. Metaphase fluorescence in situ hybridization analysis using probes specific for 14q32, i.e. IGH rearrangements, could be performed in 57 of the 74 cases, and revealed 14q32 aberrations in 10 cases not seen by conventional G-banding. Abnormal karyotypes were detected in 77 (41%) of the 186 cultures, 46 (48%) of the 95 samples, and in 41 (55%) of the 74 patients, revealing a total of 20 chromosomal aberrations previously not reported in plasma cell dyscrasias. We found no evidence that gender, age, disease phase, culture time, or cytokine stimulation significantly influences the karyotypic features of MM.},
  author       = {Nilsson, Therese and Lenhoff, S and Turesson, Ingemar and Rylander, Lars and Mitelman, Felix and Westin, J and Höglund, Mattias and Johansson, Bertil},
  issn         = {1600-0609},
  keyword      = {disease phase,gender,age,multiple myeloma,cytogenetics,culture,conditions},
  language     = {eng},
  number       = {6},
  pages        = {345--353},
  publisher    = {Wiley-Blackwell},
  series       = {European Journal of Haematology1987-01-01+01:00},
  title        = {Cytogenetic features of multiple myeloma: impact of gender, age, disease phase, culture time, and cytokine stimulation},
  url          = {http://dx.doi.org/10.1034/j.1600-0609.2002.00724.x},
  volume       = {68},
  year         = {2002},
}