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Characterization of a glutamate biosensor based on a novel glutamate oxidase integrated into a redox hydrogel

Mikeladze, E ; Collins, Alexandra LU ; Sukhacheva, M ; Netrusov, A and Csöregi, Elisabeth LU (2002) In Electroanalysis 14(15-16). p.1052-1059
Abstract
A newly isolated and purified glutamate oxidase was used to construct amperometric biosensors for glutamate monitoring. The enzyme-producing microorganism belongs to the genus of Streptomyces - Streptomyces sp. Z-11-6 - and is capable of producing extracellular L-glutamate oxidase. The microorganism was obtained by mutagenesis with HNo2 from a parent strain isolated from soil of Lypetskaya region, Russia, followed by conventional screening methods. The developed biosensor is based on a coupled enzyme system (glutamate oxidase and horseradish peroxidase), the enzymes being crosslinked to a redox polymer (PVI19-dmeOs) using poly(ethylene glycol) diglycidyl ether as crosslinker. ne characteristics of the obtained biosensors were compared with... (More)
A newly isolated and purified glutamate oxidase was used to construct amperometric biosensors for glutamate monitoring. The enzyme-producing microorganism belongs to the genus of Streptomyces - Streptomyces sp. Z-11-6 - and is capable of producing extracellular L-glutamate oxidase. The microorganism was obtained by mutagenesis with HNo2 from a parent strain isolated from soil of Lypetskaya region, Russia, followed by conventional screening methods. The developed biosensor is based on a coupled enzyme system (glutamate oxidase and horseradish peroxidase), the enzymes being crosslinked to a redox polymer (PVI19-dmeOs) using poly(ethylene glycol) diglycidyl ether as crosslinker. ne characteristics of the obtained biosensors were compared with those obtained for similarly constructed electrodes based on a commercially available glutamate oxidase from Yamasa Corp., Japan. The biosensors were operated at - 50 mV (vs. Ag/AgCl 0.1 M KCl) in a flow injection system. Special attention has been focused on the selectivity of the biosensors, evaluating their responses in the presence of several potentially interfering substances, possibly present in brain microdialysates (ascorbic acid, aspartate, cysteine, dopamine, DOPAC, GABA, glutamine, glycine, 5-HIAA, HMPG, HVA, serotonin, tryptophan, tyrosine and uric acid). The biosensors based on the two enzymes displayed similar bioanalytical characteristics but different selectivity patterns. (Less)
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author
; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
novel glutamate oxidase, biosensor, selectivity
in
Electroanalysis
volume
14
issue
15-16
pages
1052 - 1059
publisher
John Wiley and Sons
external identifiers
  • wos:000177846000002
  • scopus:0036714843
ISSN
1040-0397
DOI
10.1002/1521-4109(200208)14:15/16<1052::AID-ELAN1052>3.0.CO;2-0
language
English
LU publication?
yes
additional info
The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Department of Chemistry (011001220), Analytical Chemistry (S/LTH) (011001004)
id
8f01dd26-c51e-4671-9e13-5321ee1694b6 (old id 329248)
date added to LUP
2016-04-01 17:06:34
date last changed
2021-01-06 03:27:04
@article{8f01dd26-c51e-4671-9e13-5321ee1694b6,
  abstract     = {A newly isolated and purified glutamate oxidase was used to construct amperometric biosensors for glutamate monitoring. The enzyme-producing microorganism belongs to the genus of Streptomyces - Streptomyces sp. Z-11-6 - and is capable of producing extracellular L-glutamate oxidase. The microorganism was obtained by mutagenesis with HNo2 from a parent strain isolated from soil of Lypetskaya region, Russia, followed by conventional screening methods. The developed biosensor is based on a coupled enzyme system (glutamate oxidase and horseradish peroxidase), the enzymes being crosslinked to a redox polymer (PVI19-dmeOs) using poly(ethylene glycol) diglycidyl ether as crosslinker. ne characteristics of the obtained biosensors were compared with those obtained for similarly constructed electrodes based on a commercially available glutamate oxidase from Yamasa Corp., Japan. The biosensors were operated at - 50 mV (vs. Ag/AgCl 0.1 M KCl) in a flow injection system. Special attention has been focused on the selectivity of the biosensors, evaluating their responses in the presence of several potentially interfering substances, possibly present in brain microdialysates (ascorbic acid, aspartate, cysteine, dopamine, DOPAC, GABA, glutamine, glycine, 5-HIAA, HMPG, HVA, serotonin, tryptophan, tyrosine and uric acid). The biosensors based on the two enzymes displayed similar bioanalytical characteristics but different selectivity patterns.},
  author       = {Mikeladze, E and Collins, Alexandra and Sukhacheva, M and Netrusov, A and Csöregi, Elisabeth},
  issn         = {1040-0397},
  language     = {eng},
  number       = {15-16},
  pages        = {1052--1059},
  publisher    = {John Wiley and Sons},
  series       = {Electroanalysis},
  title        = {Characterization of a glutamate biosensor based on a novel glutamate oxidase integrated into a redox hydrogel},
  url          = {http://dx.doi.org/10.1002/1521-4109(200208)14:15/16<1052::AID-ELAN1052>3.0.CO;2-0},
  doi          = {10.1002/1521-4109(200208)14:15/16<1052::AID-ELAN1052>3.0.CO;2-0},
  volume       = {14},
  year         = {2002},
}