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Effects of Marine Oils, Digested with Human Fluids, on Cellular Viability and Stress Protein Expression in Human Intestinal Caco-2 Cells

Tullberg, Cecilia; Vegarud, Gerd; Undeland, Ingrid and Scheers, Nathalie (2017) In Nutrients 9(11).
Abstract

In vitro digestion of marine oils has been reported to promote lipid oxidation, including the formation of reactive aldehydes (e.g., malondialdehyde (MDA) and 4-hydroxy-2-hexenal (HHE)). We aimed to investigate if human in vitro digestion of supplemental levels of oils from algae, cod liver, and krill, in addition to pure MDA and HHE, affect intestinal Caco-2 cell survival and oxidative stress. Cell viability was not significantly affected by the digests of marine oils or by pure MDA and HHE (0-90 μM). Cellular levels of HSP-70, a chaperone involved in the prevention of stress-induced protein unfolding was significantly decreased (14%, 28%, and 14% of control for algae, cod and krill oil, respectively; p ≤ 0.05). The oxidoreductase... (More)

In vitro digestion of marine oils has been reported to promote lipid oxidation, including the formation of reactive aldehydes (e.g., malondialdehyde (MDA) and 4-hydroxy-2-hexenal (HHE)). We aimed to investigate if human in vitro digestion of supplemental levels of oils from algae, cod liver, and krill, in addition to pure MDA and HHE, affect intestinal Caco-2 cell survival and oxidative stress. Cell viability was not significantly affected by the digests of marine oils or by pure MDA and HHE (0-90 μM). Cellular levels of HSP-70, a chaperone involved in the prevention of stress-induced protein unfolding was significantly decreased (14%, 28%, and 14% of control for algae, cod and krill oil, respectively; p ≤ 0.05). The oxidoreductase thioredoxin-1 (Trx-1) involved in reducing oxidative stress was also lower after incubation with the digested oils (26%, 53%, and 22% of control for algae, cod, and krill oil, respectively; p ≤ 0.001). The aldehydes MDA and HHE did not affect HSP-70 or Trx-1 at low levels (8.3 and 1.4 μM, respectively), whilst a mixture of MDA and HHE lowered Trx-1 at high levels (45 μM), indicating less exposure to oxidative stress. We conclude that human digests of the investigated marine oils and their content of MDA and HHE did not cause a stress response in human intestinal Caco-2 cells.

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author
publishing date
type
Contribution to journal
publication status
published
keywords
Animals, Aquatic Organisms/chemistry, Caco-2 Cells, Cell Survival/drug effects, Cod Liver Oil, Euphausiacea/chemistry, Gastric Juice, Gene Expression Regulation/drug effects, Humans, Oils/chemistry, Saliva, Stress, Physiological/drug effects
in
Nutrients
volume
9
issue
11
publisher
MDPI AG
external identifiers
  • scopus:85033450078
ISSN
2072-6643
DOI
10.3390/nu9111213
language
English
LU publication?
no
id
329ee622-4945-4144-9004-47c38359e7a8
date added to LUP
2019-07-01 16:26:29
date last changed
2019-09-17 04:58:06
@article{329ee622-4945-4144-9004-47c38359e7a8,
  abstract     = {<p>In vitro digestion of marine oils has been reported to promote lipid oxidation, including the formation of reactive aldehydes (e.g., malondialdehyde (MDA) and 4-hydroxy-2-hexenal (HHE)). We aimed to investigate if human in vitro digestion of supplemental levels of oils from algae, cod liver, and krill, in addition to pure MDA and HHE, affect intestinal Caco-2 cell survival and oxidative stress. Cell viability was not significantly affected by the digests of marine oils or by pure MDA and HHE (0-90 μM). Cellular levels of HSP-70, a chaperone involved in the prevention of stress-induced protein unfolding was significantly decreased (14%, 28%, and 14% of control for algae, cod and krill oil, respectively; p ≤ 0.05). The oxidoreductase thioredoxin-1 (Trx-1) involved in reducing oxidative stress was also lower after incubation with the digested oils (26%, 53%, and 22% of control for algae, cod, and krill oil, respectively; p ≤ 0.001). The aldehydes MDA and HHE did not affect HSP-70 or Trx-1 at low levels (8.3 and 1.4 μM, respectively), whilst a mixture of MDA and HHE lowered Trx-1 at high levels (45 μM), indicating less exposure to oxidative stress. We conclude that human digests of the investigated marine oils and their content of MDA and HHE did not cause a stress response in human intestinal Caco-2 cells.</p>},
  author       = {Tullberg, Cecilia and Vegarud, Gerd and Undeland, Ingrid and Scheers, Nathalie},
  issn         = {2072-6643},
  keyword      = {Animals,Aquatic Organisms/chemistry,Caco-2 Cells,Cell Survival/drug effects,Cod Liver Oil,Euphausiacea/chemistry,Gastric Juice,Gene Expression Regulation/drug effects,Humans,Oils/chemistry,Saliva,Stress, Physiological/drug effects},
  language     = {eng},
  month        = {11},
  number       = {11},
  publisher    = {MDPI AG},
  series       = {Nutrients},
  title        = {Effects of Marine Oils, Digested with Human Fluids, on Cellular Viability and Stress Protein Expression in Human Intestinal Caco-2 Cells},
  url          = {http://dx.doi.org/10.3390/nu9111213},
  volume       = {9},
  year         = {2017},
}