Development of a Molecular Imprinting-Based Surface Plasmon Resonance Biosensor for Rapid and Sensitive Detection of Staphylococcus aureus Alpha Hemolysin From Human Serum
(2020) In Frontiers in cellular and infection microbiology 10.- Abstract
Stapylococcus aureus is a common infectious agent in e.g. sepsis, associated with both high mortality rates and severe long-term effects. The cytolytic protein α-hemolysin has repeatedly been shown to enhance the virulence of S. aureus. Combined with an unhindered spread of multi drug-resistant strains, this has triggered research into novel anti virulence (i.e. anti α-hemolysin) drugs. Their functionality will depend on our ability to identify infections that might be alleviated by such. We therefore saw a need for detection methods that could identify individuals suffering from S. aureus infections where α-hemolysin was a major determinant. Molecular imprinted polymers were subsequently prepared on gold coated sensor chips. Used in... (More)
Stapylococcus aureus is a common infectious agent in e.g. sepsis, associated with both high mortality rates and severe long-term effects. The cytolytic protein α-hemolysin has repeatedly been shown to enhance the virulence of S. aureus. Combined with an unhindered spread of multi drug-resistant strains, this has triggered research into novel anti virulence (i.e. anti α-hemolysin) drugs. Their functionality will depend on our ability to identify infections that might be alleviated by such. We therefore saw a need for detection methods that could identify individuals suffering from S. aureus infections where α-hemolysin was a major determinant. Molecular imprinted polymers were subsequently prepared on gold coated sensor chips. Used in combination with a surface plasmon resonance biosensor, α-hemolysin could therethrough be quantified from septic blood samples (n = 9), without pre-culturing of the infectious agent. The biosensor recognized α-hemolysin with high affinity (KD = 2.75 x 10-7 M) and demonstrated a statistically significant difference (p < 0.0001) between the α-hemolysin response and potential sample contaminants. The detection scheme proved equally good, or better, when compared to antibody-based detection methods. This novel detection scheme constitutes a more rapid, economical, and user-friendly alternative to many methods currently in use. Heightening both reproducibility and sensitivity, molecular imprinting in combination with surface plasmon resonance (SPR)-technology could be a versatile new tool in clinical- and research-settings alike.
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- author
- Andersson, Tilde LU ; Bläckberg, Anna LU ; Lood, Rolf LU and Ertürk Bergdahl, Gizem LU
- organization
- publishing date
- 2020
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- alpha hemolysin, molecular imprinting, sepsis, SPR, Staphylococcus aureus
- in
- Frontiers in cellular and infection microbiology
- volume
- 10
- article number
- 571578
- publisher
- Frontiers Media S. A.
- external identifiers
-
- scopus:85097279413
- pmid:33330120
- ISSN
- 2235-2988
- DOI
- 10.3389/fcimb.2020.571578
- language
- English
- LU publication?
- yes
- id
- 32ae4433-593f-4fce-b497-44204f0fcb25
- date added to LUP
- 2020-12-15 11:11:59
- date last changed
- 2024-05-30 01:21:38
@article{32ae4433-593f-4fce-b497-44204f0fcb25,
abstract = {{<p>Stapylococcus aureus is a common infectious agent in e.g. sepsis, associated with both high mortality rates and severe long-term effects. The cytolytic protein α-hemolysin has repeatedly been shown to enhance the virulence of S. aureus. Combined with an unhindered spread of multi drug-resistant strains, this has triggered research into novel anti virulence (i.e. anti α-hemolysin) drugs. Their functionality will depend on our ability to identify infections that might be alleviated by such. We therefore saw a need for detection methods that could identify individuals suffering from S. aureus infections where α-hemolysin was a major determinant. Molecular imprinted polymers were subsequently prepared on gold coated sensor chips. Used in combination with a surface plasmon resonance biosensor, α-hemolysin could therethrough be quantified from septic blood samples (n = 9), without pre-culturing of the infectious agent. The biosensor recognized α-hemolysin with high affinity (K<sub>D</sub> = 2.75 x 10<sup>-7</sup> M) and demonstrated a statistically significant difference (p < 0.0001) between the α-hemolysin response and potential sample contaminants. The detection scheme proved equally good, or better, when compared to antibody-based detection methods. This novel detection scheme constitutes a more rapid, economical, and user-friendly alternative to many methods currently in use. Heightening both reproducibility and sensitivity, molecular imprinting in combination with surface plasmon resonance (SPR)-technology could be a versatile new tool in clinical- and research-settings alike.</p>}},
author = {{Andersson, Tilde and Bläckberg, Anna and Lood, Rolf and Ertürk Bergdahl, Gizem}},
issn = {{2235-2988}},
keywords = {{alpha hemolysin; molecular imprinting; sepsis; SPR; Staphylococcus aureus}},
language = {{eng}},
publisher = {{Frontiers Media S. A.}},
series = {{Frontiers in cellular and infection microbiology}},
title = {{Development of a Molecular Imprinting-Based Surface Plasmon Resonance Biosensor for Rapid and Sensitive Detection of Staphylococcus aureus Alpha Hemolysin From Human Serum}},
url = {{http://dx.doi.org/10.3389/fcimb.2020.571578}},
doi = {{10.3389/fcimb.2020.571578}},
volume = {{10}},
year = {{2020}},
}