¹H and hyperpolarized ³He magnetic resonance imaging clearly detect the preventative effect of a glucocorticoid on endotoxin-induced pulmonary inflammation in vivo
Olsson, Lars E LU
; Smailagic, Amir
; Onnervik, Per-Ola
; Lindén, Anders
and Hockings, Paul D
(2011)
In Innate Immunity
17(2).
p.11-204
- Abstract
INTRODUCTION: Proton (¹H ) magnetic resonance imaging (MRI) can be utilized to quantify pulmonary edema in endotoxin-induced pulmonary inflammation and hyperpolarized (HP) ³He MRI can assess pulmonary ventilation. Neither of the methods has been applied to assess the impact of a drug on endotoxin-induced pulmonary inflammation in vivo. The aim of the current study was to evaluate the capability of ¹H and HP ³He MRI to assess the effects of a glucocorticoid on endotoxin-induced pulmonary inflammation in vivo.
MATERIALS AND METHODS: Mice were exposed to an aerosol of either saline or endotoxin (5 mg/ml) for 10 min. Half of the endotoxin-exposed mice were pretreated with a glucocorticoid (budesonide 3 mg/kg; 2 times/day) and the... (More)
INTRODUCTION: Proton (¹H ) magnetic resonance imaging (MRI) can be utilized to quantify pulmonary edema in endotoxin-induced pulmonary inflammation and hyperpolarized (HP) ³He MRI can assess pulmonary ventilation. Neither of the methods has been applied to assess the impact of a drug on endotoxin-induced pulmonary inflammation in vivo. The aim of the current study was to evaluate the capability of ¹H and HP ³He MRI to assess the effects of a glucocorticoid on endotoxin-induced pulmonary inflammation in vivo.
MATERIALS AND METHODS: Mice were exposed to an aerosol of either saline or endotoxin (5 mg/ml) for 10 min. Half of the endotoxin-exposed mice were pretreated with a glucocorticoid (budesonide 3 mg/kg; 2 times/day) and the other half with vehicle p.o. The first budesonide treatment was administered 1 h prior to the aerosol inhalation. Forty-eight hours after the aerosol exposure, the mice were anaesthetized for subsequent imaging. Hyperpolarized ³He was administered and axial MR images of the lungs obtained. Matching ¹H MR images were then acquired. The mice were sacrificed and broncho-alveolar lavage (BAL) samples were harvested to determine total and cell differential counts.
RESULTS: The lesion volume on both ¹H and ³He MRI, were markedly increased by endotoxin exposure (P<0.001). Budesonide strongly reduced lesion volume (P<0.001). The BAL cell count correlated strongly with both (3)He (P<0.001; r = 0.96) and ¹H lesion volumes (P<0.001; r = 0.97).
CONCLUSIONS: Hyperpolarized ³He MRI and ¹H MRI clearly visualized the preventative effect of budesonide on the impact of endotoxin on pulmonary ventilation and edema, respectively. The fact that ventilation defects on ³He MRI corresponded to findings from conventional ¹H MRI, as well as to counts of BAL inflammatory cells suggests that these imaging techniques constitute promising tools for non-invasive monitoring of pulmonary inflammation in vivo.
(Less)
- author
- Olsson, Lars E
LU
; Smailagic, Amir
; Onnervik, Per-Ola
; Lindén, Anders
and Hockings, Paul D
- publishing date
- 2011-04
- type
- Contribution to journal
- publication status
- published
- keywords
- Animals, Bronchoalveolar Lavage Fluid, Budesonide, Endotoxins, Feasibility Studies, Helium, Hydrogen, Magnetic Resonance Imaging, Mice, Mice, Inbred BALB C, Pneumonia, Respiratory Function Tests
- in
- Innate Immunity
- volume
- 17
- issue
- 2
- pages
- 8 pages
- publisher
- SAGE Publications
- external identifiers
-
- pmid:20130108
- scopus:79953694794
- ISSN
- 1753-4267
- DOI
- 10.1177/1753425909359191
- language
- English
- LU publication?
- no
- id
- 32d75e5b-8d59-4903-b53a-af16ef3e5484
- date added to LUP
- 2016-08-16 12:10:40
- date last changed
- 2024-01-04 10:47:10
@article{32d75e5b-8d59-4903-b53a-af16ef3e5484,
abstract = {{<p>INTRODUCTION: Proton (¹H ) magnetic resonance imaging (MRI) can be utilized to quantify pulmonary edema in endotoxin-induced pulmonary inflammation and hyperpolarized (HP) ³He MRI can assess pulmonary ventilation. Neither of the methods has been applied to assess the impact of a drug on endotoxin-induced pulmonary inflammation in vivo. The aim of the current study was to evaluate the capability of ¹H and HP ³He MRI to assess the effects of a glucocorticoid on endotoxin-induced pulmonary inflammation in vivo.</p><p>MATERIALS AND METHODS: Mice were exposed to an aerosol of either saline or endotoxin (5 mg/ml) for 10 min. Half of the endotoxin-exposed mice were pretreated with a glucocorticoid (budesonide 3 mg/kg; 2 times/day) and the other half with vehicle p.o. The first budesonide treatment was administered 1 h prior to the aerosol inhalation. Forty-eight hours after the aerosol exposure, the mice were anaesthetized for subsequent imaging. Hyperpolarized ³He was administered and axial MR images of the lungs obtained. Matching ¹H MR images were then acquired. The mice were sacrificed and broncho-alveolar lavage (BAL) samples were harvested to determine total and cell differential counts.</p><p>RESULTS: The lesion volume on both ¹H and ³He MRI, were markedly increased by endotoxin exposure (P<0.001). Budesonide strongly reduced lesion volume (P<0.001). The BAL cell count correlated strongly with both (3)He (P<0.001; r = 0.96) and ¹H lesion volumes (P<0.001; r = 0.97).</p><p>CONCLUSIONS: Hyperpolarized ³He MRI and ¹H MRI clearly visualized the preventative effect of budesonide on the impact of endotoxin on pulmonary ventilation and edema, respectively. The fact that ventilation defects on ³He MRI corresponded to findings from conventional ¹H MRI, as well as to counts of BAL inflammatory cells suggests that these imaging techniques constitute promising tools for non-invasive monitoring of pulmonary inflammation in vivo.</p>}},
author = {{Olsson, Lars E and Smailagic, Amir and Onnervik, Per-Ola and Lindén, Anders and Hockings, Paul D}},
issn = {{1753-4267}},
keywords = {{Animals; Bronchoalveolar Lavage Fluid; Budesonide; Endotoxins; Feasibility Studies; Helium; Hydrogen; Magnetic Resonance Imaging; Mice; Mice, Inbred BALB C; Pneumonia; Respiratory Function Tests}},
language = {{eng}},
number = {{2}},
pages = {{11--204}},
publisher = {{SAGE Publications}},
series = {{Innate Immunity}},
title = {{¹H and hyperpolarized ³He magnetic resonance imaging clearly detect the preventative effect of a glucocorticoid on endotoxin-induced pulmonary inflammation in vivo}},
url = {{http://dx.doi.org/10.1177/1753425909359191}},
doi = {{10.1177/1753425909359191}},
volume = {{17}},
year = {{2011}},
}