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Nanoparticle effect on neutrophil produced myeloperoxidase

Sanfins, Elodie LU ; Correia, Alexandra; Gunnarsson, Stefan B. LU ; Vilanova, Manuel and Cedervall, Tommy LU (2018) In PLoS ONE 13(1).
Abstract

Nanoparticles affect the immune system as they may interact directly with immune cells and activate them. However, it is possible that nanoparticles also interact with released cytokines and immunologically active enzymes. To test this hypothesis, the activity of myeloperoxidase released from activated neutrophils was measured in the presence of nanoparticles with different chemistry and size. In high concentrations of nanoparticles, myeloperoxidase activity is decreased whereas in low concentrations of nanoparticles the activity is increased. The effect of the nanoparticles on myeloperoxidase is dependent on the total protein concentration as low concentrations of bovine serum albumin together with nanoparticles further increase the... (More)

Nanoparticles affect the immune system as they may interact directly with immune cells and activate them. However, it is possible that nanoparticles also interact with released cytokines and immunologically active enzymes. To test this hypothesis, the activity of myeloperoxidase released from activated neutrophils was measured in the presence of nanoparticles with different chemistry and size. In high concentrations of nanoparticles, myeloperoxidase activity is decreased whereas in low concentrations of nanoparticles the activity is increased. The effect of the nanoparticles on myeloperoxidase is dependent on the total protein concentration as low concentrations of bovine serum albumin together with nanoparticles further increase the myeloperoxidase activity. The results herein show that nanoparticles affect the immune response not only at the cellular level but also on released immune effectors. In particular, they show that the nanoparticle effect on myeloperoxidase activity in the neutrophil degranulation environment is the result of an intricate interplay between the enzyme and protein concentrations in the environment and the available surface area on the nanoparticle.

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author
organization
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type
Contribution to journal
publication status
published
subject
in
PLoS ONE
volume
13
issue
1
publisher
Public Library of Science
external identifiers
  • scopus:85040767982
ISSN
1932-6203
DOI
10.1371/journal.pone.0191445
language
English
LU publication?
yes
id
32e0e14c-f3b8-4c37-a778-30eb0208f257
date added to LUP
2018-01-30 14:45:06
date last changed
2018-01-31 03:00:03
@article{32e0e14c-f3b8-4c37-a778-30eb0208f257,
  abstract     = {<p>Nanoparticles affect the immune system as they may interact directly with immune cells and activate them. However, it is possible that nanoparticles also interact with released cytokines and immunologically active enzymes. To test this hypothesis, the activity of myeloperoxidase released from activated neutrophils was measured in the presence of nanoparticles with different chemistry and size. In high concentrations of nanoparticles, myeloperoxidase activity is decreased whereas in low concentrations of nanoparticles the activity is increased. The effect of the nanoparticles on myeloperoxidase is dependent on the total protein concentration as low concentrations of bovine serum albumin together with nanoparticles further increase the myeloperoxidase activity. The results herein show that nanoparticles affect the immune response not only at the cellular level but also on released immune effectors. In particular, they show that the nanoparticle effect on myeloperoxidase activity in the neutrophil degranulation environment is the result of an intricate interplay between the enzyme and protein concentrations in the environment and the available surface area on the nanoparticle.</p>},
  articleno    = {e0191445},
  author       = {Sanfins, Elodie and Correia, Alexandra and Gunnarsson, Stefan B. and Vilanova, Manuel and Cedervall, Tommy},
  issn         = {1932-6203},
  language     = {eng},
  month        = {01},
  number       = {1},
  publisher    = {Public Library of Science},
  series       = {PLoS ONE},
  title        = {Nanoparticle effect on neutrophil produced myeloperoxidase},
  url          = {http://dx.doi.org/10.1371/journal.pone.0191445},
  volume       = {13},
  year         = {2018},
}