Advanced

Compaction of DNA by gemini surfactants: Effects of surfactant architecture

Karlsson, L; van Eijk, M and Söderman, Olle LU (2002) In Journal of Colloid and Interface Science 252(2). p.290-296
Abstract
The interaction between bacteriophage T4 DNA and cationic gemini surfactants was studied by the use of fluorescence microscopy. Upon addition of surfactant, DNA undergoes a transition from random coil to globule, with an intermediate coexistence region. The state behavior of a DNA-gemini surfactant system was found to depend on spacer length, valency, head group size, and tail length. A series of alkanediyl-alpha,omega-bis-(dimethylalkylammonium bromide) surfactants with fixed tail length and variable spacer length s showed a minimum of compaction efficiency at s = 6 due to the competition between entropy loss and enthalpy gain. This occurs at roughly the same spacer length at which the critical micellization concentration shows a maximal... (More)
The interaction between bacteriophage T4 DNA and cationic gemini surfactants was studied by the use of fluorescence microscopy. Upon addition of surfactant, DNA undergoes a transition from random coil to globule, with an intermediate coexistence region. The state behavior of a DNA-gemini surfactant system was found to depend on spacer length, valency, head group size, and tail length. A series of alkanediyl-alpha,omega-bis-(dimethylalkylammonium bromide) surfactants with fixed tail length and variable spacer length s showed a minimum of compaction efficiency at s = 6 due to the competition between entropy loss and enthalpy gain. This occurs at roughly the same spacer length at which the critical micellization concentration shows a maximal value (at s = 5). In comparison with a single-tailed divalent surfactant (12-3-1) it was shown that the two-tailed equivalent (12-3-12) was more efficient in compacting DNA. A series of gemini surfactants based on cationic peptides with a alpha,omega-diamino alkyl spacer showed similar behavior upon changing the spacer length. Additionally, two surfactants based on diastereomers of tartaric acid with hexadecanoic acid tails and alpha,omega-diaminopropanyl and spermidine head groups, respectively, showed effects of head group size that depended strongly on entropy effects. The dependence on valency of the head group is found to be similar to what is known for mono- and multivalent ions, the latter being more efficient per unit of charge. (C) 2002 Elsevier Science (USA). (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
compaction, DNA, gene therapy, gemini surfactant, DNA-surfactant interaction
in
Journal of Colloid and Interface Science
volume
252
issue
2
pages
290 - 296
publisher
Elsevier
external identifiers
  • wos:000177509500005
  • scopus:0036967755
ISSN
1095-7103
DOI
10.1006/jcis.2002.8477
language
English
LU publication?
yes
id
cbda1b6d-c816-4af5-949d-7938c04321a2 (old id 331015)
date added to LUP
2007-10-11 07:47:32
date last changed
2017-08-20 03:36:33
@article{cbda1b6d-c816-4af5-949d-7938c04321a2,
  abstract     = {The interaction between bacteriophage T4 DNA and cationic gemini surfactants was studied by the use of fluorescence microscopy. Upon addition of surfactant, DNA undergoes a transition from random coil to globule, with an intermediate coexistence region. The state behavior of a DNA-gemini surfactant system was found to depend on spacer length, valency, head group size, and tail length. A series of alkanediyl-alpha,omega-bis-(dimethylalkylammonium bromide) surfactants with fixed tail length and variable spacer length s showed a minimum of compaction efficiency at s = 6 due to the competition between entropy loss and enthalpy gain. This occurs at roughly the same spacer length at which the critical micellization concentration shows a maximal value (at s = 5). In comparison with a single-tailed divalent surfactant (12-3-1) it was shown that the two-tailed equivalent (12-3-12) was more efficient in compacting DNA. A series of gemini surfactants based on cationic peptides with a alpha,omega-diamino alkyl spacer showed similar behavior upon changing the spacer length. Additionally, two surfactants based on diastereomers of tartaric acid with hexadecanoic acid tails and alpha,omega-diaminopropanyl and spermidine head groups, respectively, showed effects of head group size that depended strongly on entropy effects. The dependence on valency of the head group is found to be similar to what is known for mono- and multivalent ions, the latter being more efficient per unit of charge. (C) 2002 Elsevier Science (USA).},
  author       = {Karlsson, L and van Eijk, M and Söderman, Olle},
  issn         = {1095-7103},
  keyword      = {compaction,DNA,gene therapy,gemini surfactant,DNA-surfactant interaction},
  language     = {eng},
  number       = {2},
  pages        = {290--296},
  publisher    = {Elsevier},
  series       = {Journal of Colloid and Interface Science},
  title        = {Compaction of DNA by gemini surfactants: Effects of surfactant architecture},
  url          = {http://dx.doi.org/10.1006/jcis.2002.8477},
  volume       = {252},
  year         = {2002},
}